Labshake search
Citations for Illumina :
1401 - 1450 of 1472 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... USA) for short read sequencing as specified in the TruSeq DNA PCR-Free Reference Guide (Oct 2017, Illumina, San Diego, CA, USA). A library was prepared using a TruSeq DNA PCR-Free library preparation kit according to manufacturer guidance and was sequenced on a NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Sequence libraries of the V1 and V3 region of the 16S rRNA genes from each of the samples were constructed in accordance with the Dual Barcoded Two-Step PCR procedure from Illumina (Illumina, 2013). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... A single library from these samples (Acerv FL 14120) was prepared using the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and the remaining samples were prepared using the TruSeq DNA Nano kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... pooled and then purified using AMPure beads before running index-PCR using the Nextera XT Index Kit (Illumina, San Diego, CA, USA). AmpliSeq libraries were subsequently normalized before sequencing the libraries as 300bp paired-end reads on Illumina MiSeq (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Genome libraries for short-read sequencing were prepared with the TruSeq DNA PCR-Free Sample Prep Kit (Illumina, San Diego, CA, USA), and sequenced on the NextSeq 500 platform (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Illumina sequencing adapters and dual-index barcodes were added to the purified amplicon products using limited cycle PCR and the Nextera XT Index Kit (Illumina, CA, USA). Amplicons from 96 samples and controls were pooled in equimolar amounts and the resultant libraries were purified by gel extraction and quantified (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... and PCR and sequencing were performed as described previously (2 x 250bp paired-end reads, on an Illumina Miseq (Lemont, IL, USA)) [68–70] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... although sequence data for each isolate is derived from pools of individuals (except from PCR-free Illumina sequence data for S. avenae), all individuals sequenced for a given isolate are expected to contain the same two haplotypes and so sequence data can be combined to reconstruct fully phased haplotypes for each isolate.
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bioRxiv - Evolutionary Biology 2023Quote: ... insert size 550 nt) were constructed from 1.8-2 µg gDNA with the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq 2500 by the Genomics Core Facility at Pennsylvania State University ...
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bioRxiv - Molecular Biology 2023Quote: ... Agilent 2100 Bioanalyzer and ABI Step One Plus Real-Time PCR System were used following the sequencing on the Illumina HiSeqTM4000 system (Illumina, United States). Illumina sequencing was performed at the Beijing Genomics Institute (BGI-Shenzhen ...
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bioRxiv - Genomics 2023Quote: ... DNA libraries were synthesised from 3.9 μg of gDNA using the Illumina TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) and sequenced on Illumina NovaSeq 6000 platform (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA was amplified by PCR using the Nextera DNA sample preparation and indexing kits (Illumina, FC-121-1030, FC-121-1011) as follows ...
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bioRxiv - Plant Biology 2020Quote: ... A second round of PCR was used to add the dual indices with the Nextra XT Index Kit (Illumina, San Diego, CA, USA). The quality of these sequencing libraries was determined by the Qubit® 2.0 Fluorometer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR gene expression quantifications were performed using AceQ qPCR SYBR Green Mix (Vazyme Biotech) on Eco Real-Time PCR System (Illumina, San Diego, CA). RNA expression was normalized to a geometric mean of two reference genes ...
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bioRxiv - Neuroscience 2021Quote: ... with 5 ng input RNA followed by 9 cycles of PCR amplification and library preparation using the Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA). Sequencing was performed on a NovaSeq 6000 (Illumina) ...
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bioRxiv - Plant Biology 2021Quote: ... A paired-end library and four mate libraries were constructed using a TruSeq DNA PCR-Free Library Prep Kit and a Nextera Mate Pair Sample Preparation Kit (Illumina, San Diego, CA), respectively ...
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bioRxiv - Microbiology 2020Quote: ... DNA libraries for purified PCR amplicons were constructed using an Illumina TruSeq Nano DNA LT Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... we constructed a sequencing library (insert size of 500 bp) with TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA, USA) to sequence on HiSeqX (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries for short- and long-read sequencing were generated with the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA) and the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Genomics 2019Quote: ... The barcode sequencing libraries were subjected to a final bead clean-up SPRIselect reagent and quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms; KK4824). Sequencing libraries were loaded on an Illumina sequencer with 2 × 50 paired-end kits using the following read length ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries with an average size of 1.5-2 kb were tagmented and indexed during a second PCR amplification step with the Illumina Nextera XT DNA preparation kit (Illumina, FC-131-1024). Tagmentation was performed according to the manufacturer’s protocol with an input of 500 pg cDNA and amplicon tagment mix for 5 min at 55 °C ...
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bioRxiv - Epidemiology 2019Quote: ... The second round of PCR added the Illumina specific sequencing adapter sequences and unique indexes using the Nextera XT Index Kit v2 (Illumina, San Diego, CA) and KAPA HIFI HotStart Ready Mix (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Two micrograms of MDA-amplified DNA were used to generate genome libraries using a TruSeq DNA PCR-Free Kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced using a Rapid-Run on an Illumina HiSeq 2500 to obtain 100 bp paired-end reads ...
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bioRxiv - Genetics 2019Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) according to Illumina library preparation protocols ...
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bioRxiv - Genomics 2021Quote: ... The PCR products (4 nM from every single cell) were treated with the Miseq Reagent Kit v2 50 Cycles (Illumina KK, Tokyo, Japan) and sequenced by the MiSeq sequencer (26 bp ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of MDA-amplified DNA were used to generate genome libraries using a TruSeq DNA PCR-Free Kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced using a Rapid-Run on an Illumina HiSeq 2500 to obtain 100 bp paired-end reads ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products (4 nM from every sample) were treated with the MiSeq Reagent Kit v2 (50 cycle format; Illumina KK, Tokyo, Japan) and sequenced using the MiSeq Sequencer (26 bp ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Neuroscience 2022Quote: ... at the HIHG or The American Genome Center at Uniformed Services University of the Health Sciences (USUHS) using the TruSeq DNA PCR-Free library preparation kit and sequencing on the Novaseq 6000 (Illumina, San Diego, CA). WGS was analyzed through a bioinformatics pipeline including alignment to the GRCh38 genome build with BWA mem ...
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bioRxiv - Genomics 2022Quote: ... Libraries were created from DNA of the same HzStark_Cry1AcR male using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit with TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA) to yield a standard paired end library with a 350±50 bp insert size ...
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bioRxiv - Microbiology 2022Quote: ... China) for PCR amplification of the V4 region of the 16S rRNA gene and sequencing using an Illumina NovaSeq (Illumina, San Diego, CA) in two sequencing runs ...
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bioRxiv - Immunology 2024Quote: ... Sequencing of the PCR amplicons spanning the MHC loci was performed on the Illumina MiSeq platform using a v2 500-cycle kit (Illumina, San Diego, CA) in the FHCC Genomics Core Facility or the UCI-Fred Hutch Cancer Centre Laboratory.
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bioRxiv - Genomics 2023Quote: ... The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030).
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries of individual DNA samples were prepared using the Illumina TruSeq DNA PCR-free LP kit (Illumina, San Diego, California, United States) in combination with the IDT for Illumina TruSeq DNA UD indexes kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation is performed with or without amplification (Nextera® DNA Flex Library Prep kit or TruSeq® DNA PCR-Free kit, ILLUMINA®), either through automation (MICROLAB Star® ...
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bioRxiv - Cell Biology 2023Quote: ... Editing frequencies in samples collected following transplantation were assessed via deep sequencing of PCR amplified libraries of the CD33 gRNA target region (Table S1) on an Illumina Miseq (Illumina, San Diego, CA) via 300bp paired end reads and a median sequencing depth of 100,000 reads/gRNA target site ...
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bioRxiv - Genomics 2020Quote: ... fragment libraries (average fragment sizes of ∼560 bp) were constructed using TruSeq DNA PCR-Free LT Library Prep Kit (Illumina, San Diego, CA, USA). Paired-end sequences of 150 bp long were determined using HiSeq X (Illumina) ...
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bioRxiv - Genomics 2020Quote: A total of 2 μg of intact genomic DNA was fragmented and used to construct short-insert PCR-free libraries (300 and 500 bp) following the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA). The DNA libraries were used in paired-end sequencing (∼265 million paired-end reads ...
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bioRxiv - Molecular Biology 2019Quote: The extracted genomic DNA from a laboratory-reared individual was used in constructing a high-throughput sequencing library with 500-bp insert size using the Illumina TruSeq DNA PCR-Free HT Library Prep Kit (Illumina, San Diego, CA, USA). The prepared library was sequenced on an Illumina Hiseq4000 Sequencer using the Hiseq Reagent Kit v3 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... An average 350-bp paired-end libraries were prepared from extracted genomic DNA by TruSeq DNA PCR-Free High Throughput Library Prep Kit (Illumina, San Diego, CA, USA). Pair-end sequencing (2× 150-bp ...
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bioRxiv - Plant Biology 2021Quote: Libraries for PCR-free short read sequencing were prepared from MN106-Ref genomic DNA using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, San Diego, CA, USA) in combination with TruSeq DNA Single Indexes Set A (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... All the libraries were prepared following the two-step PCR Illumina protocol and these were subsequently sequenced on an Illumina MiSeq instrument (Illumina, San Diego, CA, USA) using 2×251 paired-end reads for the MICROWINE samples and 2×301 paired-end reads for the Biome Makers samples.
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bioRxiv - Genomics 2021Quote: ... Eight whole genome sequencing libraries with a median insert size of 550 bp were constructed using the Illumina TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR free Library Prep Kit (Illumina, San Diego, CA, USA) and size selected at about 400 bp ...
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bioRxiv - Molecular Biology 2019Quote: ... and 16 PCR positive controls) was sequenced on an Illumina MiSeq® using a MiSeq Reagent Kit v3 (600-cycle) (Illumina, Inc, CA, USA). Raw sequence reads were demultiplexed using a custom Python script ...
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bioRxiv - Microbiology 2019Quote: ... the amplicon libraries underwent a second PCR reaction to attach dual indices and Illumina sequencing adapters using the Nextera XT index kit (Illumina, San Diego, CA, USA). Following purification (as described above ...
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bioRxiv - Genomics 2021Quote: ... libraries with an average insert size of 450 bp were prepared by the University of Utah’s Huntsman Cancer Institute High-Throughput Genomics Core Facility using the Illumina TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, California, USA). 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...