Labshake search
Citations for Illumina :
1101 - 1150 of 1399 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... A paired-end library was constructed using a TruSeq DNA PCR-Free Library Prep kit (Illumina, CA, USA) and size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... sequencing of PCR product libraries was performed on the Illumina MiSeq platform (Illumina Inc, San Diego, CA, USA). The paired-end (PE ...
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bioRxiv - Microbiology 2023Quote: ... by the manufacturer’s protocol (DNA input ≥100 ng) but using TruSeq DNA PCR-free CD index 20015949 (Illumina) adapters to eliminate PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... amplification and PCR products index labeling adapted from the bacterial 16s rRNA Metagenomic sequencing library preparation (Illumina, USA). The previously designed primers were modified for NGS analysis adding the following overhang adaptors to the 5’-prime end of each forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were then sent to Biofidal sequencing company for purification and 2×300bp Miseq sequencing (Illumina). A total of 12,836,921 and 14,248,382 reads were obtained from 16S and 18S rDNA respectively and were demultiplexed using the Mothur pipeline (69 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were quantitated with qPCR (NEBNext Library Quant Kit for Illumina, Bio-rad CFX96 Touch Real-Time PCR). Libraries were normalized to 1.5 pM and pooled ...
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bioRxiv - Cell Biology 2019Quote: ... The sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824) and Qubit 3.0 with dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: Short-read sequence libraries for JI4 and JI128 were prepared using the TruSeq DNA PCR-Free Library Kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... sgRNA libraries were generated using two-step PCR and sequenced by paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Immunology 2021Quote: ... and amplicons were used to perform the index PCR using the Nextera XT index v2 (Illumina #FC-131-1002). The index PCR products were cleaned with the Agencourt AMPure XP kit (Beckman Coulter #A63881 ...
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bioRxiv - Genomics 2019Quote: ... Library preparation was performed following the Illumina Truseq DNA PCR-Free LT Library Prep Kit (Illumina, San Diego, CA) protocol using 1ug of isolated genomic DNA ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were then tagged using Nextera XT DNA Library Preparation Kit (Cat. 15032354, Illumina, San Diego, CA) and indexed using Nextera XT Index Kit (Cat ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were prepared for sequencing on the HiSeq2500 platform with the TruSeq DNA PCR-free Library Prep Kit (Illumina). In total 83 P ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated from1 µg of genomic DNA using the TruSeq® DNA PCR-free Library Prep Kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA from these cells was used for PCR amplification of sgRNAs and sequenced using a MiSeq system (Illumina). Fold depletion or enrichment of sgRNAs from the NGS data was calculated using PinAplPy software38.
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bioRxiv - Immunology 2021Quote: ... PCR products were verified on 1.2% agarose gels and then indexed in a 2nd round PCR reaction using the Nextera Index kit (Illumina). PCR2 conditions for indexing were ...
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bioRxiv - Microbiology 2019Quote: ... The resulting PCR products were purified and loaded onto the Illumina MiSeq cartridge (Illumina Inc., San Diego, Ca, USA).
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bioRxiv - Immunology 2022Quote: ... PCR products were purified and paired-end sequenced using the MiSeq v3 reagent kit (Illumina, San Diego, CA, USA). Reads with quality score of ≥ 30 and mean quality score ≥ 35 were further processed ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were generated using the TruSeq DNA PCR-Free library prep kit (Illumina Inc., San Diego, CA, USA) and sequenced on an Illumina MiSeq instrument at 2 x 151 paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... Next generation libraries were generated using the TruSeq DNA PCR Free Nano kit (Illumina, Inc., San Diego, CA, USA) and the ARTIC multiplex PCR genome amplification protocol with the V3 primer scheme (www.protocols.io/view/ncov-2019-sequencing-protocol-bbmuik6w ...
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bioRxiv - Microbiology 2022Quote: ... The second round of PCR followed the manufacturer’s guidelines for 16S rRNA Metagenomic Sequencing Library protocol (Illumina, Cat.: 150442223) for the MiSeq system ...
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bioRxiv - Genomics 2022Quote: ... Libraries of 550 bp inserts were constructed using Truseq DNA PCR-Free kit and sequenced on NextSeq500 (Illumina, USA). Raw reads were filtered as described above ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR reactions were sent to the @Bridge platform (INRAe, Jouy-en-Josas, france) for sequencing (Illumina Miseq technology). Single multiplexing was performed using home-made 6 bp index ...
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bioRxiv - Cancer Biology 2022Quote: ... The first PCR aims to amplify genotyping fragments prior to sample indexing and uses P5 (binds the P5 Illumina sequencing handle ...
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bioRxiv - Microbiology 2022Quote: ... The H37Rv samples were library prepped using an Illumina DNA PCR-free library prep kit (Illumina, San Diego, USA) (Supplementary table 1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Clean PCR amplicons from the same DNA sample were pooled to generate 137 libraries that were sequenced by Illumina MiSeq v3 ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by limited PCR enrichment to generate the final cDNA sequencing library (Nextera® XT DNA Library Prep, Illumina). Libraries were sequenced as single-end 75 base pair reads on the Illumina NextSeq500 per the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... All sequencing followed the protocol in the Illumina TruSeq PCR-Free Sample Preparation Guide (Illumina cat# FC-121-2001), and used PCR-free library preparation kits purchased from KAPA Biosystems (see https://www.nhlbiwgs.org/topmed-whole-genome-sequencing-project-freeze-5b-phases-1-and-2 for additional details) ...
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bioRxiv - Genetics 2020Quote: ... Serial PCRs were performed for targeted deep sequencing and then a MiniSeq sequencing platform (Illumina, San Diego, CA, USA) was used for next generation sequencing (NGS) ...
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bioRxiv - Genomics 2021Quote: ... to prepare PCR-free paired-end libraries using the Illumina Genomic DNA Sample Preparation kit following the manufacturer’s instructions (Illumina). All paired-end libraries were sequenced on an Illumina NovaSeq 6000 system ...
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bioRxiv - Genomics 2020Quote: ... 600 pg of PCR product was prepared into Illumina sequencing libraries through tagmentation with a Nextera XT kit (Illumina). Tagmentation was performed according to manufacturer’s instructions and the library was amplified with primers Truseq5 and N700 series barcoded index primers ...
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bioRxiv - Genomics 2021Quote: ... The purified PCR products were then subjected to a multiplexing process using Nextera XT Index kit (Illumina, California, USA) in 50 μL reactions ...
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bioRxiv - Genomics 2019Quote: ... 415 out of 427 libraries were prepared the TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA). For the remaining 12 libraries ...
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bioRxiv - Microbiology 2022Quote: We sequenced the pooled sample containing PCR products using Illumina MiSeq technology (Illumina Reagent Kit v2, 500 reaction kit) at the Center for Bioinformatics and Genomics at Indiana University ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product was purified in a 1 % agarose gel and sequenced using a NextSeq 550 and NovaSeq (Illumina) to obtain 79+79-nt ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed with 12 PCR cycles and the rest of the library preparation was performed according to the manufacturer’s instructions (10X Genomics, Illumina). All libraries were sequenced on an Illumina NovaSeq 6000 using S4 to a target sequencing depth of 100,000 reads/cell ...
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bioRxiv - Genomics 2022Quote: A short-read sequence library of ‘Takanotsume’ was prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) and sequenced on the NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was amplified for library construction with PCR using Nextera i5 common adaptor and i7 index adaptors (Illumina). The final libraries were sequenced on Illumina HiSeq 4000.
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bioRxiv - Microbiology 2022Quote: ... Paired-end sequencing (2×150 bp) and PCR-free whole genome sequencing was performed on a HiSeq X (Illumina) 54 ...
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bioRxiv - Bioengineering 2022Quote: ... Pooled PCR products were sequenced on the MiSeq using v2 2×250 base pair kit (Illumina, San Diego, CA). Sequences were processed in Mothur v ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Cancer Biology 2023Quote: ... indexed libraries for human WGS were constructed from 1.0 μg of gDNA using the TruSeq DNA PCR-Free Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then purified and sequenced on Illumina HiSeq 2500 platform (Illumina Inc., San Diego, CA, USA) via a paired-end approach (2 × 250 bp) ...
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bioRxiv - Genetics 2023Quote: ... PCR-enriched junctions were deep-sequenced using next-generation sequencing (NGS) technologies (Illumina MiSeq v3 single-end 150 cycles) (Figure S5).
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bioRxiv - Evolutionary Biology 2023Quote: ... and libraries were prepared using the Illumina Truseq DNA PCR-Free Sample preparation kit (Illumina Inc., San Diego, CA). Six pools were combined into a Miseq run (PE250 ...
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bioRxiv - Synthetic Biology 2023Quote: ... sequencing libraries were prepared using a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA). The purified libraries were subjected to paired-end (2 × 150 bp ...
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bioRxiv - Microbiology 2023Quote: ... DNA-Seq libraries from 2.5 µg of genome DNA were prepared using the TruSeq DNA PCR-free kit (Illumina). Then ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were used for library construction and sequenced on the NovaSeq PE250 platform (Illumina, San Diego, USA) according to the protocol of Majorbio Bio-Pharm Technology Co ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions ...