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Citations for Illumina :
1251 - 1300 of 1399 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was amplified with 25 cycles of PCR and libraries will be prepared for sequencing with standard NexteraXT Illumina protocol (Illumina).
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bioRxiv - Physiology 2023Quote: ... The subsequent steps of library preparation were carried out on the Agilent NGS Bravo workstation in 96-well plates adapted for the TruSeq PCR-free Sample Preparation protocol by Illumina.
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bioRxiv - Plant Biology 2023Quote: ... A paired-end library with insert sizes ranging from 450 to 550 bp was constructed using the TruSeq DNA PCR-Free Library Prep kit (Illumina) and was size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research ...
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bioRxiv - Zoology 2023Quote: ... DNA fragments were used to generate genomic libraries using the TruSeq DNA PCR-free library kit (Illumina, San Diego, CA), which were quality checked on an Agilent 4200 Tapestation and quantitated using a Lightcycler qPCR assay (Roche Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Libraries for high-throughput sequencing of PCR products were prepared following the “16S Metagenomic Sequencing Library Preparation” protocol by Illumina, using primers with extensions containing the adapters and the MiSeq Reagent Kit v2 (CaBM_D219A_Fw and CaBM_D219A_Rv ...
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bioRxiv - Immunology 2023Quote: ... The V3-V4 region of 16s rDNA was amplified by PCR and sequenced in the MiSeq platform at THSTI (Illumina) using the 2 × 250 bp paired-end protocol yielding pair-end reads that overlap almost completely ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed single-nucleus indexing by dispensing 600 nL of Nextera PCR Master Mix (NPM) and 400 nL of a unique Nextera index pair (Illumina, cat ...
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bioRxiv - Cell Biology 2023Quote: ... PCR was performed for 40 cycles (15 s at 95°C and 45 s at 60°C) using a Thunderbird SYBR Green Polymerase Kit (TOYOBO) and Eco Real-Time PCR System (Illumina).
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bioRxiv - Biochemistry 2023Quote: ... Libraries were then performed by PCR with a library preparation kit (Vazyme, TD501) and sequencing was done using the Novaseq system (Illumina) according to the manufacturer’s protocol.
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... we utilized an Illumina DNA Prep kit with Nextera DNA CD Indexes to create an NGS library from the purified PCR products (Illumina). The libraries were then pooled and transferred onto a MiniSeq sequencer (Illumina).
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bioRxiv - Evolutionary Biology 2024Quote: ... which were used to construct a genomic library using the TruSeq DNA PCR-free library kit (Illumina, San Diego, CA). Library quality was assessed with an Agilent 4200 Tapestation and Lightcycler quantitative PCR (Roche Life Science ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... The generated cDNA was 3′ end- adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: Illumina sequencing libraries for three amplified DNA samples were prepared using Nextera TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and paired-end libraries were constructed according to the Illumina TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina). The four paired-end libraries were quantified by qPCR using the KAPA Library Quantification Kit (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... Another cleanup step with SPRIselect was performed before the Sample Index PCR when P5 and P7 sequences (for Illumina sequencing) and sample indices (to allow for multiplexing in the sequencer ...
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bioRxiv - Genomics 2024Quote: ... The sequencing library was prepared with 1 μg of DNA using the TruSeq PCR-free DNA sample preparation kit (cat# 20015962, Illumina), according to the TruSeq DNA PCR-free Reference Guide ...
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bioRxiv - Cell Biology 2024Quote: ... At least 200□pg of amplified cDNA were used for tagmentation reaction and subsequent PCR amplification using the Nextera XT kit (Illumina) to construct sequencing libraries ...
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bioRxiv - Immunology 2024Quote: ... two extra PCR cycles were performed and full length cDNA equally spilt for short-read gene expression library generation (Illumina) and targeted V(D)J capture followed by long-read library generation (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The following index PCR for both gene libraries was conducted with the Nextera XT index kit v2 (Illumina Inc., USA). The PCR product was purified using the LabopassTM DNA purification kit (Cosmogenetech Inc. ...
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bioRxiv - Genetics 2023Quote: ... Paired-end libraries with insert sizes ranging from 300 to 500 bp were generated using the NEXTflex PCR-Free DNA Sequencing Kit (Biooscientific) or the TruSeq Nano DNA Library Prep Kit (Illumina). Libraries were quantified using the KAPA Library Quantification Kit (Cliniscience) ...
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bioRxiv - Molecular Biology 2024Quote: ... Dual indices and Illumina sequencing adapters were attached to the amplicons with index PCR using Nextera XT Index Kit v2 (Illumina). The libraries were combined with the PhiX Control v3 (Illumina) ...
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bioRxiv - Cancer Biology 2023Quote: ... Between 350 and 500ng of tumour DNA were used to prepare DNA sequencing libraries using the TruSeq DNA PCR Free 350bp kit (Illumina). The samples were then sequenced on the Illumina NovaSeq6000 sequencing machine using S4 flow cells to generate 2 x 151 paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were checked by gel electrophoresis and sequencing was performed using a MiSeq sequencer (Illumina Inc., San Diego, CA) using a 600 bp kit ...
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bioRxiv - Biochemistry 2024Quote: ... Illumina sequencing adaptors were introduced by PCR and the final library sequenced at the JHU Genetic Resources Core Facility on a HiSeq2500 (Illumina) in Rapid Mode using 50 bp single reads ...
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide containing the Illumina P7 adaptor sequence was ligated to the 3’ end of the single stranded LAM PCR fragments using Circligase (Epicentre/Illumina, #CL9025K). A P5 adaptor was added by PCR ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were then indexed and PCR amplified (10 cycles) and sequenced on Illumina HiSeq 2500 sequencing platform following the manufacture’s protocols (Illumina, Hayward CA.).Sequence reads were aligned to GRCh37-lite using Burrows-Wheeler Aligner (BWA ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... dual indexed libraries were PCR amplified and the purified PCR products were pooled in equimolar concentrations and sequenced using 150 paired end sequencing on MiSeq or NextSeq500 sequencers (Illumina USA).
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bioRxiv - Genomics 2020Quote: ... The purified PCR reactions were pooled together and then sent for paired-end Illumina MiSeq sequencing (Illumina 2 × 250 v2 kit) at the Edinburgh Genomics Facility (University of Edinburgh ...
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bioRxiv - Cancer Biology 2021Quote: ... indexed libraries for human whole genome sequencing were constructed from 1.0 µg gDNA using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Illumina barcodes and adapters were attached to pooled and purified products in a second PCR (8 cycles) with the Nextera XT Index Kit A and D (Illumina Inc.). Libraries were purified with Agencourt AMPure XP kit (Beckman coulter ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Doyle and Doyle 1987) and subjected to PCR (for Sanger sequencing) or rolling-circle amplification with the GE TempliPhi kit (for Illumina sequencing) per the manufacturer's instructions ...
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bioRxiv - Immunology 2020Quote: ... PCR amplicons were sequenced on an Illumina MiSeq instrument (v2 chemistry, 150 bp paired end reads) (Illumina, San Diego, CA, USA). Data were analyzed using a custom-built pipeline ...
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bioRxiv - Genetics 2019Quote: ... The libraries were amplified using the NPM mix (Nextera PCR Master Mix from Nextera DNA Library Prep Kit) and Index adapters i7 and i5 (Nextera Index Kit, Illumina, U.S), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... TruSeq PCR-Free Low Throughput libraries were prepared for each DNA sample following the manufacturer’s instructions (Illumina, San Diego, CA, USA). Each sample had a unique index from Illumina TruSeq DNA CD Indexes (96 indexes/samples) ...
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bioRxiv - Biochemistry 2020Quote: ... Sheared DNA was used to construct the library using the Illumina TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, 20015962). After adapter ligation ...
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bioRxiv - Plant Biology 2020Quote: ... The sequencing step of PCR fragments was done on an Illumina Miseq personal sequencer using the MiSeq Reagent Kit v3 (Illumina®) followed by quality control processes for libraries using the PippinHT system from SAGE Sciences for libraries purification ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of gDNA per sample was analyzed using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... PCR-generated amplicon libraries were subjected to 250 nt paired-end sequencing on a MiSeq platform (Illumina, San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: Short-read paired-end sequencing of DNA and rRNA-depleted samples was performed on the Illumina Novaseq platform (S prime, 2x 150 bp) using TruSeq PCR free DNA library preparation kit (Illumina Inc.).
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bioRxiv - Microbiology 2019Quote: ... The libraries generated with TruSeq DNA PCR-Free Sample Preparation Kit were sequenced using paired-end Illumina sequencing (2 × 250 bp) on the HiSeq2500 platform (Illumina, USA).
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bioRxiv - Genomics 2019Quote: ... Dovetail staff then prepared a sequencing library by inputting fragmented DNA to the TruSeq DNA PCR-Free Library Preparation Kit (Illumina, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng alkylated RNA was used and prepared with a commercially available kit (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina, Lexogen). Sequencing was performed on an Illumina NovaSeq SP platform in 100bp-single-read mode.
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bioRxiv - Evolutionary Biology 2019Quote: We assessed input gDNA quantity using Qubit and normalised the samples to 20ng/ul as described in TruSeq®DNA PCR-Free Library Prep Reference Guide (#FC-121-3001, Illumina) prior fragmentation to 350bp with Covaris S2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 500 ng of the purified and pooled PCR genome amplicons were prepared for sequencing via Nextera DNA Flex Kit (Illumina, #20018705) with 96 indexes (Illumina ...
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bioRxiv - Genetics 2019Quote: ... Illumina sequencing adapters were added by PCR using 12 cycles of amplification (Illumina Cat. FC-131-1024 and FC-131-2001). Final sequencing libraries were purified and size selected using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... or the Illumina TruSeq DNA PCR-free LT sample preparation kit (Illumina, Part no. FC-121–3001 and FC-121–3002) according to the manufacturer’s protocols with some modifications (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: We performed 8 additional cycles of PCR with Nextera 24-Index kit for indexing before sample pooling (Illumina, FC-121-1011), for which we used 7.5 μl of the above elute as template ...
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bioRxiv - Microbiology 2021Quote: ... A 50µl aliquot of the final pooled PCR product was sequenced at the UC Davis Genome Center DNA Technologies Core via the Illumina MiSeq PE250 platform (Illumina, CA).
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina, #20020492). Final library concentrations were quantified with both Qubit fluorometric quantification (DNA dsDNA HS kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1.1 micrograms of extracted DNA was utilized as starting material for TruSeq DNA PCR-Free library preparation kit (Illumina Inc., USA). The DNA was fragmented using focused ultrasonicator (Covaris M220 ...