Labshake search
Citations for Illumina :
1201 - 1250 of 1472 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: A short-read sequence library of ‘Takanotsume’ was prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) and sequenced on the NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was amplified for library construction with PCR using Nextera i5 common adaptor and i7 index adaptors (Illumina). The final libraries were sequenced on Illumina HiSeq 4000.
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bioRxiv - Microbiology 2022Quote: ... Paired-end sequencing (2×150 bp) and PCR-free whole genome sequencing was performed on a HiSeq X (Illumina) 54 ...
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bioRxiv - Molecular Biology 2024Quote: ... including PCR cycles (12 cycles for cDNA amplification of 5000 cells and 14 cycles for index PCR. E13.5GE libraries from Evf2+/+and Evf2TS/TS were sequenced on the Illumina NovaSeq 6000 (Sequencing Core Facility at the La Jolla Institute).
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bioRxiv - Microbiology 2024Quote: ... This PCR product was prepared for sequencing using the Nextera XT DNA Sample Prep Kit (Illumina, FC-131-1096) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Pooled PCR products were sequenced on the MiSeq using v2 2×250 base pair kit (Illumina, San Diego, CA). Sequences were processed in Mothur v ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Cancer Biology 2023Quote: ... indexed libraries for human WGS were constructed from 1.0 μg of gDNA using the TruSeq DNA PCR-Free Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then purified and sequenced on Illumina HiSeq 2500 platform (Illumina Inc., San Diego, CA, USA) via a paired-end approach (2 × 250 bp) ...
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bioRxiv - Genetics 2023Quote: ... PCR-enriched junctions were deep-sequenced using next-generation sequencing (NGS) technologies (Illumina MiSeq v3 single-end 150 cycles) (Figure S5).
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bioRxiv - Evolutionary Biology 2023Quote: ... and libraries were prepared using the Illumina Truseq DNA PCR-Free Sample preparation kit (Illumina Inc., San Diego, CA). Six pools were combined into a Miseq run (PE250 ...
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bioRxiv - Synthetic Biology 2023Quote: ... sequencing libraries were prepared using a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA). The purified libraries were subjected to paired-end (2 × 150 bp ...
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bioRxiv - Microbiology 2023Quote: ... DNA-Seq libraries from 2.5 µg of genome DNA were prepared using the TruSeq DNA PCR-free kit (Illumina). Then ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were used for library construction and sequenced on the NovaSeq PE250 platform (Illumina, San Diego, USA) according to the protocol of Majorbio Bio-Pharm Technology Co ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified and either analysed by Sanger sequencing (Mix2seq, Eurofins Genomics, Germany) or Next-Generation Sequencing (Illumina, NGSelect Amplicon ...
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bioRxiv - Immunology 2024Quote: ... We then searched for (TRA or TRB) PCR targets isolated using Illumina MiSeq sequencing (sequencing platform keyword: “Illumina MiSeq”); on manual inspection ...
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bioRxiv - Plant Biology 2020Quote: ... to an average size of 500-bp and indexing with sequencing adapters using the TruSeq DNA (PCR free) kit (Illumina). Sequencing was performed on a single lane of an Illumina HiSeq2500 with 500 cycles in high-output mode ...
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bioRxiv - Immunology 2021Quote: ... The ligated DNA was then barcoded and amplified by a limited cycle PCR and the barcoded Illumina libraries were sequenced by using paired-end 150-base protocol on a NextSeq 2000 (Illumina). Demultiplexed sequence reads were analyzed in the CLC Genomics Workbench v.21.0.3 by (i ...
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bioRxiv - Genetics 2021Quote: ... RNA-seq libraries were prepared from 500ng of RNA samples using an oligo dT based method with 15 cycles of PCR amplification with the Illumina TruSeq mRNA stranded kit (Illumina) and sequenced on the Illumina HiSeq 2000 v4 (50-bp single end read) ...
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bioRxiv - Microbiology 2019Quote: Two overlapping IN-PCR regions corresponding to the 288 amino acids of HIV-1 IN were sequenced with the MiSeq NGS platform (Illumina). The amplicons were purified with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... Input material ranged from 500pg to 15ng of immunoprecipitated DNA and processed according to the kit guidelines (NEBNext End Prep, Adaptor Ligation, PCR enrichment of Adaptor-Ligated DNA using NEBNext Multiplex Oligos for Illumina). Clean up and size selection were performed with AMPure beads (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... The barcode sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824). Sequencing libraries were loaded at 18 pM on an Illumina HiSeq2500 using the following read length ...
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bioRxiv - Genomics 2020Quote: ... Successful amplicons were screened on a 1.5% agarose gel and further purified using Agencourt AMPure XP PCR Purification kit before proceeding to Nextera XT DNA Library Preparation (Illumina), according to manufacture instructions ...
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bioRxiv - Genomics 2020Quote: ... DNA samples were processed using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) on the Hamilton Microlab Star (Hamilton Robotics ...
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bioRxiv - Genomics 2020Quote: ... Paired-end libraries were prepared by the Purdue Genomics Core Facility using the Truseq DNA PCR-Free Sample Preparation Kit (Illumina). Whole-genome sequencing was performed using Illumina short-read technology (2×100bp ...
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bioRxiv - Immunology 2019Quote: ... Libraries were prepared in parallel using the same number of PCR cycles and sequenced in parallel using a 150+150 bp NextSeq (Illumina).
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bioRxiv - Plant Biology 2019Quote: PCR amplification products of the V5–V7 region of fungal 18S and bacterial 16S rRNA genes were sequenced by Illumina MiSeq sequencer ...
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bioRxiv - Immunology 2019Quote: ... and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit (cat# FC-131-1096, Illumina). Sequencing of the 15 mRNAseq libraries multiplexed together was carried out with an Illumina HiSeq2500 on a 2x125 bp paired-end run.
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bioRxiv - Genomics 2019Quote: ... and three mate-pair libraries (insert sizes of 2 kb, 5 kb, and 8 kb) were constructed using the TruSeq PCR-free Kit (Illumina) and Mate-pair Kit (Illumina) ...
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bioRxiv - Genomics 2019Quote: ... Each sample library was uniquely barcoded and quantified by PCR using a PhiX Control v3 (Illumina, Cat #FC-110-3001) standard curve ...
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bioRxiv - Synthetic Biology 2020Quote: ... Adaptors were added to these PCR products and then the pooled libraries were subjected to 300 cycle single-end sequencing using MiSEQ (Illumina). Data was analyzed using Geneious 9.0.5 NGS analysis tools.
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bioRxiv - Microbiology 2019Quote: ... Paired-end sequencing (2 × 150 bp) was performed on a 300 bp PCR-free insert library on a HISeq 2500 (Illumina) at Novogene (HK ...
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bioRxiv - Genomics 2019Quote: ... and ligated to TruSeq LT adapters using a TruSeq DNA PCR-Free Library Preparation Kit (Illumina, San Diego, CA, USA). We purified the ligation reaction using a Qiaquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... Following immunoprecipitation with Dynabeads Protein G beads (invitrogen) in PCR tubes, samples were subject to Tagmentation (5 µl Tagmentation Buffer, 1 µl Tagmentation DNA Enzyme (Illumina), 19 µl Nuclease free water ...
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bioRxiv - Genomics 2021Quote: ... libraries with an insert size of 350 bp with the TruSeq DNA PCR-free gel-free library preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... resequencing of genomic DNA was performed for accessions of ‘Kirakiraboshi,’ ‘Frau Yoshimi,’ ‘Posy Bouquet Grace,’ and ‘Blue Picotee Manaslu.’ Sequencing libraries (insert size of 500 bp) for the four lines were constructed with TruSeq DNA PCR-Free Library Prep Kit (Illumina) to sequence on a HiSeqX (Illumina) ...
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bioRxiv - Genetics 2019Quote: DNA sequencing libraries were constructed from 1 µg of genomic DNA using TruSeq DNA PCR-free Library Prep kit (Illumina). Sequencing was run on an Illumina HiSeq 2500 apparatus using a paired-end read length of 2×150 pb with the Illumina Reagent Kits as already described (Demars et al ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNA sequences were recovered by genomic PCR analysis and deep sequencing using MiSeq for single-end 150-bp read length (Illumina). The primer sequences used for cloning and sequencing are listed in Supplementary Table S1 ...
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bioRxiv - Cancer Biology 2019Quote: WGS libraies were generated from 1 μg of 50 ng/ul high molecular weight gDNA using the TruSeq PCR-free Library Prep Kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina). The final libraries were purified by AmPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product mixture was sent to FASMAC to acquire 2 × 250 bp paired-end sequences using the MiSeq platform (Illumina).
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bioRxiv - Microbiology 2021Quote: ... High-throughput pyrosequencing of the PCR products was performed using the Illumina MiSeq platform (Illumina, Inc., San Diego, CA, USA). Paired-end reads of the original DNA fragments were excluded from the analysis if they did not well-match a 12-base Golay barcode (one or no errors) ...
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were prepared using 700 ng of DNA and the TruSeq DNA PCR-Free Library Prep Kit (Illumina Inc.) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of gDNA with a DNA integrity number (DIN) of <=6 was used for library preparation using the TruSeq PCR-free library preparation kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the sgRNA coding sequences integrated in the genomic DNA from the initial and 3-times selected populations were amplified by touch-down PCR and sequenced by Illumina deep sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The 9 genomic DNA pools for sequencing were prepared by mixing equimolar concentrations of DNA from which 9 libraries were prepared with an insert size of 350 bp using a TruSeq PCR-free sample prep kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... In the second method purified PCR amplicons (100 ng) were used to prepare sequencing libraries by TruSeq Nano DNA LT Library Preparation Kit (Illumina) with the following modifications ...
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...