Labshake search
Citations for Illumina :
1101 - 1150 of 1259 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... was used for reverse transcription and generation of double stranded cDNA for subsequent library preparation using the Nextera XT Library Preparation kit (Illumina, San Diego, CA). Reaction Buffer (1X ...
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bioRxiv - Neuroscience 2021Quote: 1 uL of diluted cDNA per well was used for library preparation using the Nextera XT DNA Sample Prep Kit (Illumina, #FC-131-1096). Each C1 IFC was pooled into one library ...
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bioRxiv - Genetics 2020Quote: ... Total RNA were used as templates for cDNA libraries and were prepared as poly-A selected libraries using the Truseq stranded mRNA protocol (Illumina, San Diego, CA) and then sequenced on the Illumina Novaseq platform at AGCT Core (Los Angeles ...
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bioRxiv - Immunology 2020Quote: ... The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA). The average insert size for the paired-end libraries was 300 bp (±50 bp) ...
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bioRxiv - Genomics 2020Quote: ... A total of 300 ng of RNA per sample was used to prepare cDNA libraries with a TruSeq Stranded mRNA LT Kit (Illumina, San Diego, USA). All libraries were sequenced on the HiSeq 2500 platform (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ng RNA was reverse transcribed to create a library (Smart-Seq v4 Ultra Low Input RNA / cDNA library) which was sequenced to generate 50 million read pairs (150 bp paired reads) (Illumina NovaSeq 6000 PE150). A total of 42 RNA samples processed by Novogene in two separate RNA-seq library batches were used for differential RNA expression analysis (Extended Table 7-1) ...
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bioRxiv - Developmental Biology 2023Quote: ... We amplified cDNA using a modified mcSCRB-seq protocol and produced libraries using the Illumina DNA Prep kit (Illumina, Inc, San Diego, CA) (112 ...
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bioRxiv - Genomics 2023Quote: ... about 1.5 ng purified pre-amplified cDNA sample was fragmented in four 20 μl tagmentation mix (10 μl 2x TD buffer, 5 μl Nextera XT (Illumina, FC-131-1096), and 5 μl cDNA sample ...
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bioRxiv - Molecular Biology 2022Quote: Amplified cDNA (0.25 ng) was used for preparing the sequencing library with the Nextera XT DNA Library Prep Kit (Illumina, San Francisco, CA, USA). Paired-end sequencing was performed on the MiSeq platform ...
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bioRxiv - Evolutionary Biology 2024Quote: The transcriptome was sequenced using Illumina HiSeq 2000 2x150bp platform from cDNA libraries prepared using TruSeq RNA sample prep kit v2 (Illumina, San Diego, CA) at Macrogen Inc ...
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bioRxiv - Neuroscience 2024Quote: Approximately 500 ng of total RNA was used for cDNA library preparation with oligo-dT primers following the Illumina TruSeq preparation guide (Illumina, San Diego, CA) (Mecklenburg et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 1 ng of cDNA per cell was transformed into a single-cell library using the Nextera XT DNA Library prep kit (Illumina FC-131-1096) and following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Genetics 2023Quote: ... Poly-A selection and cDNA synthesis were performed using the TruSeq Stranded mRNA kit as outlined by the manufacturer (Illumina RS-122-2103). All steps were automated on the Perkin Elmer Sciclone NGSx Workstation ...
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bioRxiv - Microbiology 2023Quote: ... cDNA libraries were prepared using Illumina TruSeq Stranded Total RNA with the Ribo-Zero Library Prep Kit (Illumina Inc., San Diego, CA USA) and RNA sequencing was performed by Psomagen (Psomagen Inc. ...
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bioRxiv - Immunology 2022Quote: ... at endpoints A and B (total 18 samples) was used to construct cDNA libraries with the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the indexed fragments were sequenced on the NGS NextSeq 500 (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... UCLA Technology Center for Genomics and Bioinformatics performed determination of the RNA quality and subsequent cDNA library preparation and sequencing (single-end reads with 75 cycles, Illumina Nextseq 500 platform). Differential expression was calculated by selecting transcripts with fold change ≤ -1.5 or ≥ 1.5 and a significance level of p <0.05 using PartekFlow ...
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bioRxiv - Genomics 2022Quote: ... 3 μg of total RNA from each sample was converted into cDNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Catalog No. RS-122-2101, Illumina, San Diego, CA) to construct cDNA libraries ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the enriched mRNA used to generate cDNA libraries with unique barcodes (Next UltraII Directional RNA Library Prep kit for Illumina, New England Biolabs). The cDNA libraries were pooled and subjected to RNA-Seq analysis at the UF-ICBR using the Illumina NextSeq 500 platform ...
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bioRxiv - Immunology 2022Quote: Approximately 200 ng total RNA was used to generate single-end cDNA libraries using TruSeq stranded mRNA Library Prep (Illumina, San Diego, CA). The libraries were sequenced using a NovaSeq 6000 Sequencing System in 100 base single reads and all samples had around 120M reads ...
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bioRxiv - Developmental Biology 2023Quote: ... 600 pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) followed by 12 cycles of library amplification ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated from the resulting cDNA (0.2ng/ul per sample) using the Nextera XT DNA library preparation kit (Illumina, Cat.no. FC-131-1024) as previously described68 ...
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bioRxiv - Genomics 2024Quote: ... Polyadenylated mRNA was strained from the total RNA and cDNA libraries were prepared using the Truseq RNA Library Prep mRNA kit (Illumina, San Diego, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... then reverse transcribed to cDNA and prepared for next-generation sequencing according to the manufacturer’s protocol (Illumina TruSeq RNA Sample Preparation v2 Guide). The cDNA libraries were validated on the Agilent 2100 Bioanalyzer using DNA-1000 chip ...
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bioRxiv - Bioengineering 2024Quote: The input samples were submitted to the Washington University Genome Technology Access Center to obtain and sequence the cDNA libaraies (Illumina NovaSeq X Plus) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: RNA from right brain hemispheres was extracted and cDNA libraries were prepared and sequenced by the University of Chicago Genomics Facility using an Illumina NovaSeqX (Illumina, San Diego, CA) with a sequencing depth of 50-60 million paired end reads per sample as described previously[45] ...
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bioRxiv - Genomics 2024Quote: ... mRNA enrichment and preparation of barcoded cDNA libraries were done using the TruSeq RNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA). F ...
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bioRxiv - Immunology 2021Quote: ... and sequencing libraries generated with a TruSeq Stranded Total RNA Human/Mouse/Rat kit (Illumina). Libraries were sequenced on a NextSeq500 platform (Illumina) ...
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bioRxiv - Pathology 2021Quote: ... Gene expression microarray analysis was performed with mouse Sentrix® BeadChips (Illumina; San Diego, CA). We followed the workflow established by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... rRNAs were depleted with the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Illumina) for human and mouse samples and with Caenorhabditis elegans Ribo-Seq riboPOOLs (siTOOLs Biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... stand-alone Ribo-Zero kits [Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)] (Illumina), Ribo-Zero accompanied by a TruSeq Stranded Total RNA Kit (Illumina) ...
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bioRxiv - Immunology 2023Quote: ... and processed and hybridized to individual array wells of an Infinium Mouse Methylation BeadChip (Illumina), as previously described 25 ...
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bioRxiv - Plant Biology 2023Quote: ... Library preparation included the “TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat”-kit (Illumina), ribosomal RNA depletion and a size selection step for 300 bp fragments ...
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bioRxiv - Microbiology 2021Quote: ... Samples were pooled for RNAsequencing of 4 nM of total cDNA and sequencing was performed using a NextSeq 500 Instrument (Illumina Inc San Diego, CA).
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bioRxiv - Neuroscience 2021Quote: ... One hundred pg of amplified cDNA was tagmented and indexed for sequencing using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA). LabChip GX Touch HT High Sensitivity assay (PerkinElmer ...
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bioRxiv - Genomics 2020Quote: Fragmented cDNA Sequencing libraries were generated from 100ng of Total RNA using NEBNext Ultra II with polyA isolation module (Illumina, San Diego, California, USA) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... library preparation was performed using the Illumina TruSeq small RNA kit and cDNA was sequenced on an Illumina NextSeq platform (50bp; Illumina, San Diego, CA, USA). Adapter sequences were trimmed with cutadapt (v2.3 ...
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bioRxiv - Developmental Biology 2021Quote: ... All cDNA libraries were pooled and sequenced to a depth of 355 million reads on a NextSeq500 machine (Illumina, 150 Cycle High Output Kit).
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bioRxiv - Cancer Biology 2021Quote: ... cDNA library synthesis and capture were performed using the Illumina TruSeq RNA Access Library Prep kit (now known as Illumina TruSeq RNA Exome kit). Amplified libraries were quantified using an automated PicoGreen assay ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately 5 ug of total RNAs were used to construct cDNA libraries (NEBNext Ultra Directional RNA Library Prep Kit for Illumina, Illumina, San Diego, CA, USA). Transcriptome sequencing was performed using the Illumina Hi-Seq 2000 platform ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA (1 μg) was then converted into cDNA libraries according to the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Illumina, San Diego, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: 4µg of total pooled RNA of all parts was taken for paired-end sequencing and prepared cDNA libraries by the use of Illumina TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, California, USA) as per it’s described protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The tagmented and multiplexed cDNA libraries were sequenced in Nextseq 500 using Nextseq 500 High Output v.2 kit (75 cycles, Illumina Cat# FC-404-2005) following Macosko et al ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA libraries were posteriorly sequenced on the Illumina Genome platform Analyzer IIx with a TruSeq kit with 36 cycles (Illumina, San Diego, CA, USA) for 72 bp paired-end reads.
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bioRxiv - Genomics 2021Quote: ... Paired-end libraries were constructed from the double stranded cDNA by using Illumina DNA Prep with Enrichment kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... The mRNA isolation, cDNA synthesis, and library preparation were performed by Novogene Corporation (Chula Vista, CA) following the standard Illumina (Illumina Inc., San Diego, CA) protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The ends of the cDNA libraries were sequenced for 60 cycles using a paired-end module on the Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA). Two biological replicates were used in each experiment.
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bioRxiv - Genetics 2023Quote: ... and 1 μg of each pool was subsequently used to build distinct cDNA libraries with the TruSeq Stranded mRNA Library Prep (Illumina, San Diego, CA, USA) kit ...
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bioRxiv - Plant Biology 2022Quote: ... Both ends of the cDNA libraries were sequenced for 60 cycles using a paired-end module with an Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA). Two biological replicates were conducted for each experiment.
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bioRxiv - Cell Biology 2022Quote: ... an average of 500 pg of amplified cDNA was used to prepare the libraries following Nextera XT DNA kit (Illumina Ref. FC-131-1096). Libraries were multiplexed by 6 on a high-output flow cells ...
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bioRxiv - Genomics 2022Quote: ... The concentration of amplified cDNA was measured with Qubit and 1 ng was used as input for Nextera XT (Illumina, San Diego, CA, USA) library preparation using New P5 SMART PCR oligo and Nextera index oligos ...