Labshake search
Citations for Illumina :
951 - 1000 of 1259 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl amplified cDNA was tagmented in 1x Tagmentation Buffer using 0.8 μl bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified fragmented cDNA of ∼200 bp in size were sequenced in paired-end mode using the HiSeq 1000 (Illumina) with a read length of 2×100 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then depleted of rRNA and converted to cDNA libraries using the TrueSeq® Stranded Total RNA kit (Illumina). cDNA libraries were sequenced with the HiSeq 4000 Sequencing System (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... poly-A selection and RNA-sequencing library preparation was done using non strand massively-parallel cDNA sequencing (mRNA-Seq) protocol from Illumina, the TruSeq RNA Library Preparation Kit v2 ...
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bioRxiv - Physiology 2023Quote: ... 1 ng of amplified cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina). Basically ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA libraries were constructed following standard protocols and paired-end sequenced on an Illumina NextSeq Sequencer (Illumina HiSeq 4000) by Novogene Bioinformatics Technology Co. ...
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bioRxiv - Immunology 2023Quote: ... Paired-end sequencing libraries were prepared from amplified cDNA with the Nextera XT DNA sample Prep Kit (Illumina, #FC-131) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The amplified cDNA was used to construct an Illumina sequencing library and sequenced on a single lane of a NovaSeq 6000 (Illumina). For FASTQ generation and alignments ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA library preparation with oligo-dT primers following the Illumina TruSeq stranded mRNA sample preparation guide (Illumina, San Diego, CA), and sequencing procedure with Illumina HiSeq 3000 platform with 30-50x106 bp reading depth were previously described in detail 19 ...
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bioRxiv - Developmental Biology 2023Quote: ... Illumina sequencing libraries were prepared from the amplified cDNA from each sample group using the Nextra DNA library prep kit (Illumina) and then sequenced on Illumina HiSeq 2500 Rapid flowcells (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... One ng cDNA was used for library preparation using Nextera XT DNA Library Preparation Kit (FC-131-1024 & FC-131- 1096, Illumina). The yield and quality of the amplified libraries were analyzed using Qubit by Thermo Fisher and the Agilent Tapestation System ...
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bioRxiv - Bioengineering 2023Quote: ... For 4-week and 8-week organoids cDNA library was prepared by using the whole transcriptome Illumina TruSeq Stranded Total RNA Library Prep Kit Gold (Illumina), followed by evaluation on an Agilent 2100 Bioanalyzer by using the DNA 1000 kit ...
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bioRxiv - Microbiology 2023Quote: ... Depletion of rRNA and cDNA library preparation were performed using lllumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina). The cDNA libraries were quantified by qPCR followed by sequencing in a NextSeq2000 equipment (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... The hashtag cDNA and endogenous cDNA libraries were diluted to 4 nM and pooled (5% hashtag + 95% endogenous cDNA) before being sequenced on a NextSeq2000 sequencer (Illumina).
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bioRxiv - Neuroscience 2023Quote: ... The cDNA library was then fragmented and indexed using the Nextera DNA Library Preparation kit (Illumina, San Diego, CA, USA). The library quality was checked on a BioAnalyzer high-sensitivity chip and concentration was quantified by Qubit assays (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... paired-end cDNA libraries were prepared from cell or EV total RNA by polyA selection using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... we prepared stranded cDNA libraries for 909 samples from 695 sepsis patients using NEB Ultra II LIbrary Prep kits (Illumina) with NEBNext Poly(A ...
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bioRxiv - Cancer Biology 2023Quote: Bead-purified cDNA pools were used for tagmentation-based library preparation with a Nextera XT DNA Library Preparation Kit (Illumina) using a custom PCR amplification strategy to generate 3’ biased libraries containing oligodT cell barcodes as previously published,60 with some modifications ...
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bioRxiv - Evolutionary Biology 2023Quote: Complementary DNA (cDNA) libraries were prepared using the Illumina TruSeq v2 mRNA sample prep kit (Illumina, San Diego, CA, USA), following a standard protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The multiplexed cDNA libraries were subjected to sequencing with 151 bp paired-end read length on a HiSeq 2500 sequencer (Illumina). The average sequencing depth was 24,861,133 paired reads per sample ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We sent 12 total RNA samples to Novogene for cDNA library prep (polyA enrichment) and paired-end 150 bp sequencing (NovaSeq PE150 on NovaSeq6000, Illumina). The samples consisted of 3 males and females from each season ...
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bioRxiv - Immunology 2024Quote: ... Library preparation was done following the CEL- Seq2 protocol69 to prepare a cDNA library for sequencing using TruSeq Small RNA primers (Illumina). The DNA library was paired-end sequenced on an Illumina NextSeq 500 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was fragmented by hydrolysis for 2 minutes at 94° and cDNA synthesis was performed with random hexamers following the TruSeq Stranded RNA kit protocol (Illumina). To achieve strand specificity ...
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bioRxiv - Plant Biology 2024Quote: ... The multiplexed cDNA libraries were then pooled (two sets of 36 libraries) and sequenced using both lanes of the NovaSeq 6000 (Illumina) S1 flow cell with run type 100 bp paired-end reads at the Genome Sequencing Service Center by the Stanford Center for Genomics and Personalized Medicine ...
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bioRxiv - Systems Biology 2024Quote: ST cDNA libraries were diluted to 4nM and 1.08pm libraries loaded for sequencing on an Illumina NextSeq 550 (Illumina, USA) using paired-end sequencing (R1 30bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using a reduced volume version of SMART-seq265 performed on an SPT Mosquito HV using 10 cycles of amplification for cDNA generation and 16 cycles for library generation using NexteraXT (Illumina). Libraries were sequenced on an Illumina NextSeq500 using paired end 38nt reads.
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion from 100 ng of total RNA and preparation of strand-specific cDNA libraries (‘fr-firststrand’) were carried out using the Total RNA Prep with Ribo-Zero Plus kit (Illumina). Quality control of the libraries was carried out using a Qubit™ dsDNA HS Assay kit and an Agilent 2100 Bioanalyzer ...
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bioRxiv - Neuroscience 2024Quote: ∼750pg of cDNA was then used as input into the Nextera XT DNA Library Preparation Kit (Illumina # FC-131-1096) with some modifications to prevent smaller fragment sizes ...
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bioRxiv - Cell Biology 2024Quote: ... library preparation was performed following the CEL-Seq2 protocol44 to prepare a cDNA library for sequencing using TruSeq small RNA primers (Illumina). The DNA library was sequenced by paired-end sequencing on an Illumina Nextseq™ 500 ...
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bioRxiv - Cell Biology 2024Quote: ... library preparation was done following the CEL-Seq2 protocol (Hashimshony, 2016) to prepare a cDNA library for sequencing using TruSeq small RNA primers (Illumina). The DNA library was paired-end sequenced on an Illumina Nextseq™ 500 ...
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bioRxiv - Genomics 2024Quote: ... 600ng of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT kit (Illumina) followed by 12 cycles of library amplification ...
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bioRxiv - Genomics 2024Quote: ... polyA-containing RNA (mRNA) was isolated and a cDNA library constructed using Stranded mRNA Prep kit as described by the manufacturer (Illumina). The quality of the cDNA library was controlled at the Finnish Functional Genomics Centre (Turku Bioscience ...
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bioRxiv - Immunology 2024Quote: ... barcoded cDNA was amplified by PCR to generate enough mass for library construction (Nextera® PCR primers) (Illumina, CA, USA). Sequencing of the libraries was performed on HiSeq2500 (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... paired-end cDNA libraries were prepared from 10 μg of total RNA by polyA selection using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The RNA was dephosphorylated in 3’ and then phosphorylated in 5’ to generate cDNA libraries using the NebNext Small RNA Sample Prep kit with 3’ sRNA Adapter (Illumina) according to the manufacturer’s protocol with 12 cycles of PCR amplification in the last step followed by DNA purification with Monarch PCR DNA cleanup kit (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA libraries were balanced by shallow iSeq and sequenced with a NovaSeq 6000 System (Illumina, S4, 2 x 100 cycles).
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bioRxiv - Genomics 2024Quote: ... cDNA was prepared using a modified protocol based on the Illumina Truseq RNA Sample Preparation Kit (Illumina, San Diego, CA) and the published methods for strand-specific RNA-Seq [66 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL amplified cDNA was tagmented in 1X Tagmentation Buffer using 0.8 µL bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Microbiology 2022Quote: ... AUS) and positive clones were validated by whole genome sequencing (WGS) on an Illumina Miseq or NextSeq550 platforms (Illumina, San Diego, CA, USA) to confirm their genotype ...
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bioRxiv - Evolutionary Biology 2024Quote: Library preparation and sequencing - DNA from 15 clones was used to prepare PCR-free paired-end sequencing libraries (Illumina DNA Prep PCR free) with an insert size of ∼300bp ...
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bioRxiv - Cell Biology 2020Quote: ... for RNA-seq library preparation (250-300 bp insert cDNA library) and sequencing (2×150 bp paired-end reads on an Illumina Platform).
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bioRxiv - Developmental Biology 2021Quote: ... was used to generate indexed 2×75bp paired-end cDNA libraries for subsequent sequencing on NextSeq 500 sequencing platform (Illumina, Inc.). Library preparation ...
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bioRxiv - Molecular Biology 2021Quote: Tagmentation with Tn5 transposase was performed on 90 ng aliquots of the cDNA library using an Illumina DNA Prep kit (Illumina, 20018704) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... A cDNA library was constructed for each sample using a TruSeq Stranded mRNA library Prep kit (Illumina, Cat# RS-122-2101) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... All libraries were prepared from 100 to 200 pg of cDNA using the Nextera XT Kit (Illumina, San Diego, California, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... then cDNA libraries were reverse-transcribed from 200□ng of extracted RNA using TruSeq Stranded Total RNA LT (Illumina® – USA), according to the manufacturer instructions ...
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bioRxiv - Physiology 2021Quote: ... cDNA was subsequently tagmented and amplified for 12 cycles by using a Nextera XT DNA Library Preparation Kit (Illumina FC-131). Sequencing libraries were analyzed with Qubit and Agilent Bioanalyzer ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... cDNA libraries were prepared using 1 μg of total RNA with the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA). The cDNA library was qualified on the Agilent Technologies 2100 Bioanalyzer to check the library quality and fragment size of the sample ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were constructed from 1 ng of pre-amplified cDNA using DNA library preparation kit (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme). Libraries were sequenced on a HiSeq-PE150 ...