Labshake search
Citations for Illumina :
1001 - 1050 of 1259 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... A total amount of 100 ng total RNA per sample was used to prepare for the rRNA-depleted cDNA library by Stranded Total RNA Prep Ligation with Ribo-Zero Plus Kit (Illumina, USA). The final library size and quality was evaluated using an Agilent High Sensitivity DNA Kit (Agilent Technologies ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... A total of 100 ng of RNA input was then used for cDNA library preparation using the TruSeq® Stranded mRNA NeoPrep kit (Illumina), according to manufacturer instructions ...
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Altered TGFB1 regulated pathways promote accelerated tendon healing in the superhealer MRL/MpJ mousebioRxiv - Cell Biology 2021Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... The pooled cDNA libraries were sequenced by a NovaSeq 6000 sequencer at 300pM final concentration for 100b paired-end sequencing (Illumina, Inc.). Approximately 30-40M reads per library were generated ...
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bioRxiv - Neuroscience 2022Quote: ... RNA samples were converted into cDNA libraries using the Illumina TruSeq Stranded Total RNA sample preparation kit (Illumina # RS-122-2303). Briefly ...
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bioRxiv - Systems Biology 2022Quote: ... Resulting cDNA libraries were single-end sequenced with a length of 50bp on an Illumina HiSeq 2000 (Illumina, San Diego, CA).
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bioRxiv - Cancer Biology 2022Quote: ... from each sample was used to isolate poly-A mRNA followed by preparation of cDNA library using the TruSeq mRNA sample preparation kit v2 (Illumina Inc.). Each sample was tagged with a unique TruSeq index tag to prepare multiplexed libraries ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA samples were processed and RNA-sequenced on a Illumina HiSeq® 2000 Sequencing platform (Illumina Inc, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The resulting cDNAs were subjected to 100 base paired-end sequencing on the Illumina HiSeq2500 Platform (Illumina, San Diego, CA, USA) (Matsumoto et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Amplified cDNA (0.125-0.375 ng) was used for generating sequencing libraries with the Illumina Nextera XT DNA kit (Illumina, FC-131-1096). All libraries were sequenced as paired-end 75 using the Illumina HiSeq4000 system.
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bioRxiv - Neuroscience 2020Quote: ... retinas of WT or Prom1-KO mice and were used to synthesise cDNA libraries with a TruSeq stranded-mRNA library preparation kit (Illumina, 20020594). The libraries were sequenced with the NextSeq 500 platform (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... The adapter-ligated library was completed by PCR with Illumina PE primers (8-11 cycles) and the resulting directional cDNA libraries were sequenced for 50 bases in a single-read format (Illumina HiSeq2000) and analyzed with nextpresso (Graña ...
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bioRxiv - Immunology 2021Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA was extracted with Trizol and the cDNA libraries (150bp, paired end reads) were sequenced using a low input protocol (Illumina Truseq) on an Illumina HiSeq2500 (Macrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-10 ng of the full-length cDNA was used as input for preparing Nextera XT (Illumina, cat. # FC-131-1024) libraries following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... cDNA library was constructed from enriched mRNA samples using the TruSeq RNA sample prep kit (Illumina, San Diego, CA, United States). rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies ...
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bioRxiv - Plant Biology 2023Quote: ... About 10 μg total RNA was used for mRNA purification followed by cDNA library preparation with the TruSeq Stranded mRNA kit (Illumina, USA). The prepared cDNA libraries were amplified by PCR and sequenced by using Illumina Hiseq 2500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Nextera libraries (5 replicates) were constructed from 0.8 ng of pre-amplified cleaned up cDNA using Nextera XT Kit (Illumina, Eindhoven, Netherlands). Index PCR was carried out using the custom P5 primer (P5NEXTPT5 ...
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bioRxiv - Cell Biology 2022Quote: ... 200ng of full-length cDNAs were used as input to the Nextera™ DNA Flex Library Prep kit (ref #20018704, Illumina) and Nextera™ DNA CD Indexes (24 Indexes ...
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bioRxiv - Bioengineering 2024Quote: ... mRNA was converted into dual-indexed cDNA libraries using the Illumina Stranded mRNA Prep and Ligation kit (Illumina; San Diego, CA) and sequenced on the Illumina NextSeq 2000 Platform (30 million clean paired-end reads per sample) ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina sequencing libraries were generated from the amplified cDNA samples using the Illumina Tagment DNA Enzyme and Buffer kit (Illumina, CA). These libraries were examined by Qubit and Bioanalyzer ...
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bioRxiv - Genetics 2023Quote: ... cDNA libraries were prepared with the TruSeq stranded mRNA library prep Kit (cat# RS-122-2101, Illumina, San Diego, CA, USA). The resulting libraries were sequenced on a HiSeq 4000 (Genomics & Cell Characterization Core Facility ...
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bioRxiv - Physiology 2023Quote: The cDNA library was then prepared from 500 ng of total RNA using the TruSeq stranded mRNA library kit (Illumina, USA) according to the manufactureŕs protocol ...
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bioRxiv - Microbiology 2023Quote: ... RNA was rRNA depleted and directional cDNA libraries were generated using either Stranded Total RNA Prep with Ribo-Zero Plus (Illumina # 20040525) or TruSeq Stranded Total RNA Ribo-Zero Gold (Illumina #RS- 122-2303) ...
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bioRxiv - Microbiology 2023Quote: ... RNA was ribominus selected and directional cDNA libraries were generated using either Stranded Total RNA Prep with Ribo-Zero Plus (Illumina # 20040525) or TruSeq Stranded Total RNA Ribo-Zero Gold (Illumina #RS-122-2303) ...
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bioRxiv - Immunology 2022Quote: ... value of the samples used for library preparation was ≥8 and cDNA libraries were prepared using IlluminaTruSeq Stranded mRNA Sample Prep kit (Illumina, USA) from 4μg of total RNA according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: The input samples were submitted to the Washington University Genome Technology Access Center to obtain and sequence the cDNA libraries (10XGenomics, 3’v3.1; Illumina NovaSeq S4) according to established protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... first and second strand cDNA synthesis were performed according to the TruSeq RNA Sample Preparation v2 Kit (Illumina RS-122-2001) using Superscript III for reverse transcription instead of Superscript II (incubation time ...
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bioRxiv - Immunology 2022Quote: ... was used to generate full-length cDNA prior to library preparation with the Nextera XT DNA Library Prep Kit (Illumina, Inc.). After pooling ...
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bioRxiv - Plant Biology 2022Quote: ... and was further processed after quality assessment and enrichment for pair-end cDNA library construction using the TruSeq RNA Library Preparation v2 Kit (Illumina, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the first strand cDNA was synthesized using TruSeq stranded total RNA kit and TruSeq stranded mRNA kit (Illumina, San Diego, USA), followed by the second strand cDNA synthesis using dUTP ...
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bioRxiv - Cancer Biology 2023Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Plant Biology 2024Quote: ... which subsequently constructed cDNA libraries for RNA-seq according to the TruSeq stranded mRNA library preparation protocol (Illumina, San Diego, USA). Sequencing was performed on a NovaSeq 6000 S4 flow cell machine (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... first and second strand cDNA synthesis were performed according to the TruSeq RNA Sample Preparation v2 Kit (Illumina, RS-122-2001) but using Superscript III for reverse transcription instead of Superscript II (50 °C for 50’ incubation time) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNA libraries of the radular tissues were prepared using TruSeq Stranded mRNA Sample Prep Kits (Illumina, San Diego, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... and cDNA libraries were generated by SeqCenter using the Stranded Total RNA Prep with Ribo-Zero Plus 563 Microbiome kit (Illumina Inc). Final libraries were sequenced on an Illumina Novaseq platform with 150 bp paired-end reads ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA underwent two rounds of PCR amplification using PrimeSTAR GXL DNA Polymerase to introduce Illumina adapters for sequencing (Illumina #20027213). The libraries were sequenced on an Illumina HiSeq or NextSeq 1000.
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bioRxiv - Genetics 2020Quote: ... or the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Genome-wide DNA methylation was analyzed using the Infinium Mouse Methylation BeadChip (Illumina). Methylation data were acquired using the iScan system and processed using GenomeStudio 2.0 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: DNA methylation was analyzed using Infinium Mouse Methylation BeadChip (MMBC) Array from Illumina. DNA for MMBC (500 ng in 40 μL nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: We used the Illumina Infinium Mouse Methylation BeadChip (Illumina, San Diego, CA, USA) to measure DNAm across the whole mouse epigenome in accordance to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... ribosomal RNA was removed using the Ribo-Zero Human/Mouse/Rat kit (Illumina). Sequencing libraries were generated according to the TruSeq stranded total RNA (Illumina ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA libraries were single-end sequenced in 76 cycles using a NextSeq 500 Kit v2 (FC-404-2005, Illumina, San-Diego, CA). High-throughput sequencing was performed using NextSeq500 sequencing system (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 140 pg of the amplified cDNA was fragmented using Nextera XT DNA sample preparation kit (Illumina FC-131-1096) and amplified with Nextera XT indexes (Illumina FC-131-1001) ...
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bioRxiv - Genomics 2020Quote: ... Complementary DNA (cDNA) libraries were constructed for each snail individual using TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were diluted and 150 pg input cDNA was used with the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) to generate indexed libraries ...
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bioRxiv - Plant Biology 2021Quote: ... Thirty cDNA libraries were constructed and sequenced on an Illumina HiSeq X ten platform in 150-bp paired-end mode (Illumina Inc., USA). Sequencing data obtained in this study have been deposited at EMBL-EBI (http://www.ebi.ac.uk/ ...
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bioRxiv - Immunology 2020Quote: ... was used to isolate total RNA that was processed to prepare cDNA libraries using TruSeq technology according to the manufacturer’s instructions protocol (Illumina, San Diego, CA). Libraries were sequenced using Illumina NovaSeq 6000 ...
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bioRxiv - Immunology 2020Quote: ... 0.1 ng of cDNA from each cell on the plate was individually tagmented using the Nextera XT kit (Illumina, San Diego, CA) in a final volume of 5 µl ...
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bioRxiv - Genetics 2021Quote: ... and prepared bar-coded cDNA libraries for sequencing on an S1 flow cell on the NovaSeq 6000 platform (Illumina, San Diego, CA) as described previously21.