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Citations for Illumina :
851 - 900 of 1259 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared our cDNA libraries for sequencing on the Illumina platform using a NexteraXT DNA Library Preparation Kit (Illumina) following the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Library preparation was done with 200 pg of cDNA employing the Nextera XT kit (Illumina, San Diego, CA, USA). Library quality and molarity were determined with the Qubit and Tapestation ...
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bioRxiv - Immunology 2024Quote: ... Advance analytical) and 0.5 ng of cDNA was used to generate indexed Illumina libraries (Nextera XT library preparation kit, Illumina). Each library’s fragment size was measured by capillary electrophoresis (Fragment analyzer ...
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bioRxiv - Systems Biology 2024Quote: ... cDNA libraries were generated from isolated RNA samples using the Total RNA Prep with Ribo-Zero Plus kit (Illumina). Barcoded libraries were sequenced on an Illumina NovaSeq instrument [150 nt paired-end reads to a depth of 1.93 × 108 ± 7.94 × 107 reads/sample (mean ± SD) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA libraries were constructed for each RNA sample using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA libraries were created from 1 µg DNase-treated RNA with the TruSeq Stranded Total RNA Globin kit (Illumina) and sequenced on the Illumina NovaSeq6000 ...
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bioRxiv - Molecular Biology 2020Quote: ... with ribosomal depletion using Human/Mouse/Rat Ribo-Zero kit (Epicentre/Illumina). All samples were sequenced using a 100 base-pair (bp ...
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bioRxiv - Genomics 2020Quote: ... Illumina TruSeq Stranded Total RNA Ribo-Zero Human/Mouse/Rat Gold (Illumina) was used to construct ribosomal RNA depleted sequencing libraries ...
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bioRxiv - Developmental Biology 2023Quote: ... TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina) was used to prepare RNA-seq libraries according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (2016) sequenced mouse whole genomes to 11-26x coverage (Illumina HiSeq 2000) from natural populations of house mouse (Mus musculus) ...
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bioRxiv - Cell Biology 2023Quote: ... RNase H or the Ribo-Zero method (human, mouse, plants) (Illumina, USA) was used to remove rRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Sperm DNA methylation was determined using Infinium Mouse Methylation BeadChip (MM285K, Illumina) which covers > 285,000 methylation sites ...
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bioRxiv - Bioengineering 2024Quote: ... TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina) was used for library construction ...
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bioRxiv - Cancer Biology 2024Quote: ... and profiled using the Infinium Mouse Methylation BeadChip (Illumina, San Diego, USA). IDAT files were imported into RnBeads and subjected to quality control ...
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bioRxiv - Cell Biology 2020Quote: ... The fragmentated mRNA samples were subjected to cDNA synthesis and library generation using the TruSeq mRNA library preparation kit (Illumina). Sequencing was performed with a NextSeq sequencer (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Peptides carried by the MCRs from sorted cells were amplified from cDNA by RT-PCR using the peptide flanking regions and sequenced on a miniSeq (Illumina). Sequences from the Illumina output files were trimmed ...
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bioRxiv - Developmental Biology 2021Quote: RNA was converted to cDNA libraries ready for sequencing using the stand-specific Script-seq V2 RNA-seq kit (Illumina). cDNAs were generated ...
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bioRxiv - Molecular Biology 2021Quote: ... A final Tapestation D100 screen was used to determine cDNA fragment size and concentration before pooling and sequencing using Hiseq2500 (Illumina).
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bioRxiv - Molecular Biology 2021Quote: ... Tagmentation of 600pg of cDNA is performed according to Nextera DNA sample preparation manufacturer instructions (Illumina, Inc., FC-131-1096) using a Truseq-P5 hybrid constant oligo (IDT ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was prepared into equimolar pools for each sample submitted to Indiana University’s Center for Genomics and Bioinformatics for cDNA library construction using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) following the standard manufacturing protocol ...
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bioRxiv - Systems Biology 2020Quote: cDNA was tagmented and amplified using the Nextera XT DNA Library Preparation Kit (FC-131-1096, Illumina, San Diego, CA). The manufacturer’s protocol was followed with the following modified reagent volumes and primers ...
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bioRxiv - Genomics 2020Quote: ... RNA was converted to cDNA and processed for NGS library prep using TruSeq Stranded Total RNA library prep kit (Illumina). Library profiles were analyzed using Bioanalyzer and sequenced on Illumina NovaSeq 6000 at a depth of 30 million reads per sample.
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bioRxiv - Developmental Biology 2020Quote: ... Adaptors for indexing samples and sequencing were added to cDNA fragmented by Tn5-mediated tagmentation using the Nextera XT kit (Illumina). Finally ...
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bioRxiv - Immunology 2020Quote: ... cDNA libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA) and the Sciclone NGSx Workstation (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... 1μg of RNA was used to prepare cDNA with the TruSeq RNA kit following the recommendations for low sample preparation protocol (Illumina). Samples were sequenced on an Illumina MiSeq instrument at a depth of 35×10^6 reads per sample ...
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bioRxiv - Genomics 2021Quote: cDNA and gene expression libraries were constructed with Smart-Seq2 chemistry and the Nextera XT DNA library prep kit (Illumina), as previously described67 ...
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bioRxiv - Neuroscience 2020Quote: Tagmentation was performed per pool by adding 3 μL double-stranded cDNA sample to 5.5 μL Nextera TD buffer (Illumina, 15027866), 2.5 μL NF H2O and 1.0 μL TDE1 Enzyme (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 1 µg of RNA per sample was used to construct the cDNA library using TruSeq stranded mRNA library prep kit (Illumina). The cDNA libraries were quantitated using KAPA library quantification kit for Illumina platforms (Kapa Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... 250 ng of total RNA was used as input for cDNA library construction with the TruSeq Stranded mRNA Sample Preparation Kit (Illumina), as per manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then fragmented and amplified for 3’ prime end sequencing with the Nextera XT DNA sample prep kit (Illumina). The libraries were purified ...
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bioRxiv - Cancer Biology 2021Quote: ... by total RNA extraction from confluent cells with Ribo-Zero rRNA depletion and DNAse treatment before cDNA was prepared with the TruSeq RNA and DNA Sample PrepKit (Illumina). 50bp paired-end cDNA libraries sequenced on an Illumina HiSeq 2000 (Genomics Core Facility ...
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bioRxiv - Plant Biology 2021Quote: ... which was enriched for mRNA and processed into cDNA libraries using the Illumina TruSeq Stranded Total RNA LT Sample Prep Kit (Illumina). After quality control (Qubit and Fragment Analyzer) ...
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bioRxiv - Systems Biology 2020Quote: ... 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina). The final libraries were purified by AmPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... RNA was isolated with the Qiagen AllPrep DNA/RNA/miRNA Universal Kit and strand specific cDNA libraries prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell and was tagmented using Nextera XT DNA Library Preparation Kit (Illumina). The tagmentation reaction was incubated at 55 °C for 8 min before removing Tn5 from the DNA by adding 0.5 µl NT buffer per well ...
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bioRxiv - Neuroscience 2020Quote: ... Full-length cDNA libraries were tagmented and amplified with Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA) according to manufacturer’s instructions with following modifications ...
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bioRxiv - Microbiology 2020Quote: 150 nucleotides of the sequence were determined from both ends of each cDNA fragment using the HiSeq 2500 platform (Illumina). An average of 83.79 million read pairs were obtained for each sample (range ...
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bioRxiv - Immunology 2020Quote: ... cDNA libraries were prepared using either the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina) or KAPA Stranded mRNA-Seq Library Prep kit (KAPA Biosytems ...
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bioRxiv - Microbiology 2020Quote: ... Seventy-five nucleotides of the sequence were determined from both ends of each cDNA fragment using the HiSeq 4000 platform (Illumina). Sequencing reads were aligned to the reference R ...
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bioRxiv - Neuroscience 2021Quote: Six hundred pg cDNA from each plate of cells was used in a modified Nextera XT (Illumina, San Diego, CA) library preparation ...
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bioRxiv - Microbiology 2021Quote: ... and further used for the cDNA synthesis by using ScriptSeq™ v2 RNA-Seq kit (Epicentre, Illumina, Madison, WI USA). The cDNA was purified using AMPure XP (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the Nova Seq 6000 (Illumina) with a read length of 100 bp ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 2 μg of total RNA was used to subselect poly(A)+ transcripts and generate strand-specific cDNA libraries (TrueSeq kit; Illumina).
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bioRxiv - Genetics 2022Quote: ... RNAs were sent to the genomic platform GeT-Plage (Toulouse, France) for the cDNA library preparation (TruSeq Stranded mRNA kit, Illumina) and the sequencing (NovaSeq 6000 Sequencing System ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA pools were sequenced using the NextSeq 500 system (HighOutput flow cell, 400 M, 1x 75 cycle single-end; Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... Illumina sequencing libraries were prepared from the PCR-amplified cDNA from mutant lungs and controls using the Nextra DNA library prep kit (Illumina). Libraries were sequenced on a NovaSeq 6000 S Prime flowcell (Illumina ...
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bioRxiv - Cell Biology 2022Quote: The iROND samples and embryonic RNA samples were reverse transcribed into cDNA libraries using the TruSeqTM RNA Sample Preparation Kit (Illumina). RNA sequencing was performed with an Illumina HiSeq™ 3000 or HiSeq X Ten platform ...
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bioRxiv - Neuroscience 2021Quote: ... ~600 pg of purified cDNA was used to produce a RNA-seq library using Nextera XT DNA Library Preparation kit (FC-131-1024, Illumina), following the manufacturer instructions.
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bioRxiv - Cell Biology 2021Quote: ... library construction (250-300 bp insert cDNA library) and RNA sequencing (Illumina HiSeq X Ten, paired-end, 10 M reads). Quality controls were included for the total RNA samples and during the procedures for RNA-sequencing.
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bioRxiv - Immunology 2020Quote: ... Use of 250 pg of cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer ...