Labshake search
Citations for Illumina :
9651 - 9681 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The libraries were prepared with Ampliseq Library Plus for Illumina kit (Illumina, San Diego, CA, USA and using Illumina’s Ampliseq CD indexes set A(Illumina), and their quality was assessed with High Sensitivity DNA reagents on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Microbiology 2024Quote: ... The pool was run at a final concentration of 10pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001) ...
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bioRxiv - Developmental Biology 2020Quote: ... This service utilizes the Ovation RNA-Seq System V2 kit (Nugen) for initial RNA amplification prior to library preparation and sequencing (Illumina HiSeq v4, 50bp paired-end reads). For each genotype ...
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bioRxiv - Evolutionary Biology 2020Quote: ... modified with 5’-overhangs designed to match the Illumina sequencing adapters and molecular identifiers (MIDs) of the Nextera® XT v2 Index Kit (Illumina Inc., San Diego, CA, USA). The second library included eight technical duplicates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All of the subsequent steps were performed by the CRG Genomics Unit (Barcelona): sequencing libraries were prepared from the pure high molecular weight DNA using TruSeq DNA HT Library Preparation kit (Illumina ® HiSeq ® Sequencing v4 Chemistry). A paired-end library with a target insert size of ∼500 bp was sequenced on an Illumina® HiSeq2500 platform in paired-end mode ...
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bioRxiv - Developmental Biology 2021Quote: ... We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN) before proceeding with Library preparation (NEBNext® Ultra™ RNA Library Prep Kit, Illumina, San Diego, CA, USA). Each sample was sequenced in Illumina HiSeq 2000 (Single read ...
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bioRxiv - Microbiology 2022Quote: The 16S and ITS libraries were prepared by the sequencing facility using the Illumina TruSeq≡Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, United States), llumina MiSeq was run using a v2 Standard sequencing format with paired end reads (2 x 250 bp) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μl of each Nextera XT Index Kit v2 set B or set C barcoding primer (Illumina, Cat# FC-131-2002 or FC-131-2003). These reactions were then incubated at 95°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... WGS libraries were sequenced with paired-end 150 base pair reads on an Illumina NovaSeq 6000 by the UW Biotechnology Center (Illumina NovaSeq 6000 S1 Reagent Kit v1.5).
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bioRxiv - Cell Biology 2019Quote: ... transcripts were purified from total RNA and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS- 122-2102). The detailed protocol for RNA sequencing was discussed in earlier (34 ...
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bioRxiv - Microbiology 2019Quote: ... A paired-end 250-bp sequencing run was performed using the Illumina MiSeq instrument according to the MiSeq™ Reagent Kit v2 Preparation Guide (Illumina, Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... and short read sequencing using the Illumina platform (Illumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000 producing 2×151bp reads) was performed by the Microbial Genome Sequencing Center ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared from samples that met the Illumina TruSeq® Stranded Total RNA Sample Preparation Guide (15031048 E) input guidelines using the Illumina TruSeq® Stranded Total (Plant) RNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for each sample were prepared from ≥200 ng of sheared DNA with TruSeq® Nano DNA High Throughput Library Prep Kit (20015965, Illumina Inc., San Diego, CA, USA) and IDT for Illumina – TruSeq® DNA UD Indexes v1 (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... we processed 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina, Cat # 20020595; Indexes: IDT for Illumina TruSeq RNA UD Indexes, cat # 20020591). The resulting libraries were then analyzed on a TapeStation (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were generated with Illumina Stranded mRNA Prep kit and sequencing of 2×50 bp paired-end reads was performed on an Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, United States). The sequences were aligned to the human GRCh38 reference genome using STAR (58) ...
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bioRxiv - Genomics 2022Quote: ... Libraries for Illumina sequencing were generated with NEBNext® Ultra™ RNA Library Prep Kit for Illumina and sequenced on the Illumina NovaSeq6000 (Illumina, Inc., San Diego, CA, USA). Libraries for long-read sequencing were generated with the cDNA PCR sequencing kit (SQK-PCS109 ...
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bioRxiv - Immunology 2019Quote: ... The fragmented mRNA samples were subjected to cDNA synthesis using Illumina TruSeq® RNA sample preparation kit version 2 (Illumina, #RS-122-2001 and #RS-122-2002) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NextSeq 500 system using the NextSeq 500/550 Mid Output Kit v2.5 (#20024904, Illumina; 150 cycles, paired-end run 2x 75 bp) with an average of 1×107 reads per RNA sample.
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bioRxiv - Cancer Biology 2023Quote: ... Triplicates were pooled for a target total cell number of 60,000 in 50uL to load less than 20uL into the 10X controller or perform TDE1-based tagmentation reactions on small volumes (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). The 10X Genomics Chromium Platform was used to generate a targeted 5,000 single cell Gel Bead-In-Emulsions (GEMs ...
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bioRxiv - Cancer Biology 2023Quote: ... The library preparation for bulk tumors and cell lines was generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc., San Diego, RS-122-2101/2) according to the manufacturer’s instructions (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... we prepared n=40 sequencing libraries from 1µg total RNA per sample using Illumina® Stranded mRNA Prep kits with unique 5′ and 3′ index adapter pairs (IDT for Illumina® RNA UD Indexes, Set A). Libraries were PCR amplified for 10 cycles ...
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bioRxiv - Microbiology 2020Quote: ... Samples that met the Illumina sample input guidelines were prepared according the TruSeq® Stranded mRNA Sample Preparation Guide (Rev. E) using the Illumina® TruSeq® Stranded mRNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Genetics 2020Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Stranded mRNA LT Sample Prep Kit – sets A and B (Illumina, Cat. No. RS-122-2101 and RS-122-2102). Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NovaSeq 6000 system using the NovaSeq 6000 S4 Individual Lane Loading Reagent kit (#20028313, Illumina; 150 cycles, paired end run 1x 111 bp) with an average of 2×107 reads per sample ...
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bioRxiv - Cancer Biology 2022Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... was performed on an Illumina NextSeq machine using either a 300 cycle v2 mid output or high output kit (Illumina, Code FC-404-2003 or Code FC-404-2004) under standard Illumina® procedure as previously described[9].