Labshake search
Citations for Illumina :
9251 - 9300 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated from the resulting cDNA (0.2ng/ul per sample) using the Nextera XT DNA library preparation kit (Illumina, Cat.no. FC-131-1024) as previously described68 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for high-throughput sequencing were constructed from 50 to 100 ng total DNA using the TruSeq Nano DNA LT library preparation kit (Illumina, San Diego, USA) for an insert size of 350 base pairs (bp ...
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bioRxiv - Microbiology 2023Quote: ... Resulting libraries were sequenced as 28/10/10/50 base reads on the NovaSeq instrument using the S2 Reagent kit in paired-end mode (Illumina, San Diego, CA). Fastq files were processed with Spaceranger-1.4.2 pipeline ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were constructed following steps from the manufacturer’s instructions (VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina®, Vazyme, NR604-02), followed by paired-end (2x 150bp ...
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Differences in gut metagenomes between dairy workers and community controls: a cross-sectional studybioRxiv - Genomics 2023Quote: Sequencing libraries were prepared from 250pg gDNA with a quarter reaction workflow using the Nextera XT Library Prep Kit (Illumina, San Diego, CA) and 12 cycles of indexing PCR ...
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bioRxiv - Systems Biology 2023Quote: ... greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep Kit with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA-seq library was constructed from the total extracted RNA using the Illumina TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Poly(A)-enriched mRNA sequencing libraries were prepared using an Illumina TruSeq® stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and sequencing reagents were used throughout the process using NEBNext Ultra DNA Library Prep kit following the manufacturer’s recommendations (Illumina, San Diego, CA, USA). End repaired adapters were ligated after adenylation of the 3’ends followed by enrichment by limited cycle PCR ...
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bioRxiv - Cell Biology 2023Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75Lcycles) (Illumina, #FC-404-2005). The library loading concentration was 2.4 pM ...
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bioRxiv - Molecular Biology 2023Quote: ... we used one lane of a NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles) (Illumina, San Diego, CA, USA, 20028401) (Illumina, San Diego, CA, USA) (1×100 bp) ...
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bioRxiv - Genomics 2023Quote: ... A sequencing library was constructed with an insert length of 300 bp using the Illumina TruSeqTM DNA Sample Preparation Kit (Illumina, San Diego, USA) following the manufacturer’s recommendations and sequenced on the MGISEQ-2000RS platform in a 150×150 bp paired-end run ...
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bioRxiv - Bioengineering 2023Quote: ... Coates Genomics Sequencing Laboratory at UC Berkeley using the 300 PE MiSeq platform and TruSeq SBS Kit v3-HS (Illumina, San Diego, CA). Illumina FASTQ files were demultiplexed ...
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bioRxiv - Neuroscience 2023Quote: ... We produced libraries for 31-mRNA sequencing using 200 ng of purified RNA per sample processed with a TruSeq Stranded RNA Kit (Illumina, San Diego, CA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... a total of 0.75 ng of DNA was combined with 2.5 µl 1/35th diluted transposome (Illumina; purchased in kit # FC-121-1011), and 1X Tris Buffer (10X Tris Buffer ...
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bioRxiv - Physiology 2023Quote: ... Each donor library pool was sequenced twice using the Illumina NextSeq500 with NextSeq 500/550 High Output v2 or v2.5 75-cycle kits (Illumina, USA; FC-404-2005). Libraries were sequenced using the following cycle parameters ...
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bioRxiv - Immunology 2023Quote: ... Samples were randomly grouped and run with two libraries per sequencing run on three NextSeq High Output 150 cycle reagent kits (Illumina, San Diego, CA), with 1% PhiX ...
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bioRxiv - Microbiology 2023Quote: ... One hundred ng of input RNA was first depleted of ribosomal RNA using an Illumina Ribo-Zero plus rRNA Depletion Kit (Illumina, San Diego, US) following Illumina’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... The library was prepared with PCR products pooled in equimolar amounts following the manufacture’s protocol and loaded in a Micro MiSeq Reagent Kit v2 (500-cycles) (Illumina, San Diego, CA, USA) on a MiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA libraries were prepared using Illumina TruSeq Stranded Total RNA with the Ribo-Zero Library Prep Kit (Illumina Inc., San Diego, CA USA) and RNA sequencing was performed by Psomagen (Psomagen Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing was performed on an Illumina MiSeq instrument using the 300-cycle MiSeq Reagent Kit v2 (Illumina, San Diego, CA, USA, MS102-2002).
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bioRxiv - Cell Biology 2023Quote: ... were used instead of the primers that came with the SMARTer Stranded Total RNA HI Mammalian kit to allow for sequencing on the NovaSeq (Illumina, San Diego, CA) by the Biopolymers Facility at Harvard Medical School.
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bioRxiv - Genomics 2023Quote: ... and it was subsequently sequenced using an Illumina Genome Analyzer IIx with the TruSeq SBS 36-Cycle Kit (Illumina, San Diego, CA, USA) for 72 bp PE reads ...
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bioRxiv - Genetics 2023Quote: ... Poly-A selection and cDNA synthesis were performed using the TruSeq Stranded mRNA kit as outlined by the manufacturer (Illumina RS-122-2103). All steps were automated on the Perkin Elmer Sciclone NGSx Workstation ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing was performed on the Illumina HiSeq 4000 (single end 125 bp) using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Genomics 2023Quote: ... Isolated nuclei were lysed and transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030). Transposed DNA was then purified on Diapure columns (Diagenode Cat# C03040001) ...
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bioRxiv - Immunology 2023Quote: ... scRNA-seq was completed at Stanford Functional Genomics Facility (SFGF) using the Chromium Single Cell Gene Expression kit (10X Genomics, CA, USA) and Illumina Novaseq (Illumina Inc, CA, USA) sequencer according to manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... Separate libraries for the two experimental conditions belonging to three independent experiments were prepared with the TruSeq RNA Sample Prep kit (Illumina, San Diego, CA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... paired-end libraries were prepared from total RNA by ribosomal depletion using the Ribo-Zero Gold plant rRNA removal kit (Illumina, San Diego, CA). Samples were processed following the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed by following a previous protocol that optimizes reagent usage for microbial genomes [85] with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) and sequenced on an Illumina HiSeq 2500 (UNH ...
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bioRxiv - Cancer Biology 2023Quote: RNA libraries were prepared from 550 ng of total RNA from tumour samples using the TruSeq Stranded Total RNA HT kit with Ribo-Zero Gold (Illumina, San Diego, USA) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... values qualified for a Poly-A enrichment strategy in order to generate the sequencing libraries applying the TruSeq mRNA Stranded Library Prep Kit (Illumina, Inc, California, USA). After Poly-A selection using Oligo-dT beads the mRNA was reverse-transcribed into cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... up to 12 samples were pooled equimolar and paired-end reads 2×150bp sequencing on an Illumina NextSeq 500 instrument using the 300 cycle Mid Output v2.5 Reagent Kit (Illumina, San Diego, CA, USA) according to Illumina guidelines ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA-Seq libraries were generated from 150-300 ng of total RNA using TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was then amplified using 10 cycles and subsequently (0.33 ng) used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina, Cat# FC-131-1096). Finally ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were used for metagenomic library preparation as described previously (32) using Illumina Nextera DNA Library Preparation Kit (Illumina, San Diego, CA) and sequenced with 2×150 bp paired-end reads on a NovaSeq platform (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... 4 μg total RNA was used to prepare RNA-seq libraries using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, US). Strand specific paired-end mRNA sequencing was performed on DNBSEQ platform at the Next-Generation Sequencing Core of the BGI Genomics Service (Hong Kong ...
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bioRxiv - Molecular Biology 2023Quote: ... 200ng mRNA per sample was used for RNA-seq library preparation using the TruSeq RNA Library Prep Kit v2 (Illumina RS-122-2001) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA solutions of approximately ≥5 ng μL−1 were subjected to the tagmentation reaction using the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA) was used at 1/40 scale of the provided protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... An initial amount of DNA ranging from 300 to 500 ng was used for library construction using all standard kit reagents from the Illumina DNA prep (M) Tagmentation (Illumina, San Diego, CA), altogether with Illumina commercial indexes (IDT for Illumina DNA/RNA UD Indexes set A ...
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bioRxiv - Genomics 2024Quote: ... Libraries were generated using the Illumina Ribo-Zero Plus rRNA Depletion Kit with IDT for Illumina RNA UD Indexes (Illumina, San Diego, CA). All samples were processed according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Polyadenylated mRNA was strained from the total RNA and cDNA libraries were prepared using the Truseq RNA Library Prep mRNA kit (Illumina, San Diego, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Illumina short-read sequencing libraries were prepared with 1 μg of high-quality RNA and the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were sent to Norwegian sequencing center (NSC) for 2x150 bp library preparation using Illumina Nextera DNA library preparation kit (Illumina, San Diego, USA) followed by sequencing on Illumina NovaSeq S4 Flowcell (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Purified libraries were sequenced on Illumina MiSeq platform (reagent kits: v2 300-cycles, paired- end mode) at 8 pM loading concentration with 25% PhiX spike-in (Illumina FC-110-3001). Custom sequencing primers were spiked into reagent cartridge (well 12 ...
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bioRxiv - Genetics 2024Quote: ... Pooled libraries were diluted and processed for either 75-bp single-end or 35-bp paired-end sequencing on an Illumina NextSeq 500 instrument (ID: NB501524) using the NextSeq 500 High Output v2 kit (75 cycles) (Illumina; FC-404-2005) in accordance with the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... and we prepared short-insert (350 bp) paired-end libraries using the TruSeq Nano DNA Library Preparation Kit (Illumina, San Diego, California, USA). We validated the fragment length distribution of the libraries with a fragment analyzer (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... Each of the libraries were sequenced on the Illumina NextSeq 500 generating paired-end 151 bp reads using approximately 31% of the NextSeq 500 High-Output v2.5 Reagent Kit (300-cycle) (Illumina, San Diego, CA, USA).