Labshake search
Citations for Illumina :
9201 - 9250 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Library preparation and small RNA sequencing of the PVH and ARC samples was performed as described previously (40) using a TruSeq® Small RNA Library Preparation kit (Illumina, Cambridge, UK). Libraries were sequenced using a HiSeq 4,000 platform ...
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bioRxiv - Neuroscience 2024Quote: ... Individually barcoded libraries were pooled and sequenced on a NextSeq mid-output paired-end sequencing run at 2×75 using NextSeq 500/550 Mid-Output v2.5 kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed using Pair-end 2×100 cycle mode on the Illumina NovaSeq 6000 system (NovaSeq 6000 S1 Reagent Kit v1.5 200 cycles Illumina, cat. no. 20028317; 0.5% of the PhiX control library, Illumina, cat. no. FC-110-3001) and the standard clustering procedure.
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bioRxiv - Immunology 2024Quote: ... TRB sequencing was performed on pools of libraries from 11 - 15 tumor or skin samples on the Illumina MiSeq platform using a v3 150-cycle kit (Illumina, San Diego, CA) in the FHCC Genomics Core Facility (Seattle ...
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bioRxiv - Microbiology 2024Quote: Total RNA of 5 μg was used to construct strand-specific RNA-sequencing libraries using the TruSeq RNA sample preparation Kit from Illumina (San Diego, CA). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 1 ng of cDNA per cell was transformed into a single-cell library using the Nextera XT DNA Library prep kit (Illumina FC-131-1096) and following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... library construction was immediately carried out using a Chromium Single Cell 3’ Reagent Kit (Version 3) and sequenced on an Illumina HiSeq 4000 or an Illumina NextSeq2000 (Illumina, cat no. 20040559) by the Technion Medicine Faculty Azrieli-Technion Genomics Center ...
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bioRxiv - Developmental Biology 2024Quote: ... >7 passed quality control and were used for library preparation with the Illumina Stranded mRNA Library Prep Kit according to manufacturer’s instructions (Cat #20040532, Illumina Inc., San Diego, CA). This procedure includes mRNA purification and fragmentation ...
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bioRxiv - Microbiology 2024Quote: ... DNAse-treated RNA extracts from Large RNA fractions were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Paired-end sequencing was performed on an Illumina sequencer according to the manufacturer instructions (PairedEnd DNA sample Prep Kit – Illumina – PE-930-1001). The custom-made adapters were removed from the reads using cut-adapt ...
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bioRxiv - Microbiology 2024Quote: DNA samples were sequenced at the Center for Quantitative Life Sciences at Oregon State University (Corvallis, OR) on an Illumina HiSeq platform using a Nextera XT library preparation kit (Illumina, San Diego, CA). Primer and adaptor removal and read-trimming were performed with fastP using default settings for Nextera adapters (35) ...
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bioRxiv - Microbiology 2024Quote: We whole-genome sequenced each sample with Illumina technology (MiSeq and HiSeq 2500) using Nextera XT library preparation kits as instructed by the manufacturer (Illumina, San Diego, CA). Fastq files (raw sequencing data ...
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bioRxiv - Molecular Biology 2024Quote: ... Paired-end sequencing was carried out on an Illumina MiniSeq system according to the manufacturer’s instructions using a 300-cycle Mid or 300-cycle High kit (Illumina, San Diego, CA, USA), depending on the number of samples in each pool ...
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing was performed on an Illumina MiSeq instrument using the 300-cycle MiSeq Reagent Kit v2 (Illumina, San Diego, CA, USA, MS102-2002).
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bioRxiv - Developmental Biology 2023Quote: ... values qualified for a Poly-A enrichment strategy in order to generate the sequencing libraries applying the TruSeq mRNA Stranded Library Prep Kit (Illumina, Inc, California, USA). After Poly-A selection using Oligo-dT beads the mRNA was reverse-transcribed into cDNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed by following a previous protocol that optimizes reagent usage for microbial genomes [85] with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) and sequenced on an Illumina HiSeq 2500 (UNH ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA-Seq libraries were generated from 150-300 ng of total RNA using TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... scRNA-seq was completed at Stanford Functional Genomics Facility (SFGF) using the Chromium Single Cell Gene Expression kit (10X Genomics, CA, USA) and Illumina Novaseq (Illumina Inc, CA, USA) sequencer according to manufacturer’s protocols.
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bioRxiv - Genetics 2023Quote: ... Poly-A selection and cDNA synthesis were performed using the TruSeq Stranded mRNA kit as outlined by the manufacturer (Illumina RS-122-2103). All steps were automated on the Perkin Elmer Sciclone NGSx Workstation ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing was performed on the Illumina HiSeq 4000 (single end 125 bp) using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was then amplified using 10 cycles and subsequently (0.33 ng) used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina, Cat# FC-131-1096). Finally ...
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bioRxiv - Genomics 2023Quote: ... Isolated nuclei were lysed and transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030). Transposed DNA was then purified on Diapure columns (Diagenode Cat# C03040001) ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were prepared with a plexWell™ 96 kit (seqWell) and sequenced on an Illumina MiSeq platform (Illumina, Inc., San Diego, CA) using a v3 reagent kit yielding 2 x 301 bp paired-end reads totaling 6.4 Gbp of sequence ...
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bioRxiv - Neuroscience 2022Quote: ... Library preparation was performed with 50ng of RNA using the TruSeq PolyA+ Stranded mRNA Library Prep Kit High Throughput (Illumina, RS-122-2103). Libraries were quality-checked on a Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Molecular Biology 2023Quote: ... The pooled library was sequenced on a MiSeq Illumina platform via 2×150 nt paired-end sequencing using the 300 cycle v2 kit (Illumina, #MS-102-2002).
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bioRxiv - Microbiology 2023Quote: ... RNA samples with RQNs ranging from 8 to 10 were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2023Quote: The sequencing library was prepared using the TruSeq Stranded mRNA Sample Prep Kit and sequenced on NovaSeq 6000 (Illumina, San Diego, CA, USA), yielding more than 6G bases of sequence for each sample ...
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bioRxiv - Microbiology 2023Quote: ... The V3-V4 region of the 16S ribosomal RNA gene was amplified by PCR with universal bacterial primer sets (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ and 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3’) and was sequenced using MiSeq Reagent kit v3 (600 cycle) (Illumina Inc., California, US). The sequence data were analyzed using QIIME2 (https://qiime2.org/ ...
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bioRxiv - Immunology 2023Quote: ... Approximately 1 ug of total RNA was used to remove ribosomal RNA according to the manuscript of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). Following purification ...
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bioRxiv - Microbiology 2023Quote: ... the short-read sequencing was done on Illumina NextSeq 550 platform using NextSeq 500/550 Mid-Output kit v2.5 (Illumina Inc, San Diego, USA) at a commercial sequencing facility (SA Pathology ...
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bioRxiv - Microbiology 2023Quote: ... were sequenced by Next-Generation Sequencing (NGS) on the Illumina MiSeq Platform using the Illumina COVIDSeq Kit for library construction (Illumina, San Diego, USA) generating paired-end reads 150 bp long ...
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bioRxiv - Genomics 2022Quote: ... The DNA paired-end libraries were constructed using either a Nextera DNA Flex or KAPA hyper library prep kit (Illumina, San Diego, USA); the RNA paired-end libraries were constructed using a TruSeq Stranded mRNA library prep kit (Illumina ...
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bioRxiv - Immunology 2023Quote: ... 100,000 nuclei per sample were isolated using an iodixanol gradient and ATAC-seq was subsequently performed according to the original protocol (Buenrostro et al, 2013) using Nextera DNA Sample preparation kit (Illumina, FC-121-1030). After library amplification ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA-seq libraries were generated using NEB Ultra II stranded mRNA chemistry (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®), as per the manufacturers protocol with the following settings ...
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bioRxiv - Bioengineering 2023Quote: ... Coates Genomics Sequencing Laboratory at UC Berkeley using the 300 PE MiSeq platform and TruSeq SBS Kit v3-HS (Illumina, San Diego, CA). Illumina FASTQ files were demultiplexed ...
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bioRxiv - Genomics 2023Quote: ... a total of 0.75 ng of DNA was combined with 2.5 µl 1/35th diluted transposome (Illumina; purchased in kit # FC-121-1011), and 1X Tris Buffer (10X Tris Buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control and quantification of the resulting dual-indexed barcoded libraries was performed with Agilent TapeStation and by qPCR (NEBNext Library Quant Kit for Illumina, New England Biolabs).
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bioRxiv - Cancer Biology 2023Quote: ... of libraries were pooled and subjected to sequencing of approximately 20 million reads per sample using the Mid Output v2 kit (Illumina, FC-404-2001) on a Illumina NextSeq550 following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Illumina sequencing libraries with 6-bp single indices were constructed from 1 μg total RNA using the TruSeq Stranded mRNA LT kit (Illumina, RS-122-2101). The resulting library was validated using the Standard Sensitivity NGS Fragment Analysis Kit (AATI ...
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bioRxiv - Genomics 2023Quote: ... The RNA library was prepared using TruSeq Stranded Total RNA Library Preparation kit with the Ribo-Zero Plant workflow (Illumina Inc., CA, USA). The transcriptome library was sequenced on Illumina NovaSeq 6000 instrument to generate 150 bp paired-end reads.
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were prepared at the Genomics Technology Core at the University of Missouri using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA). Approximately 35 million pair-end sequences of 100 nucleotides long were produced per sample in a NovaSeq6000 instrument (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Samples with an RNA integrity number of > 9 were used for construction of a complementary DNA library by an Illumina TruSeq kit (Illumina, San Diego, CA). The sequencing strategy was 100 bp paired-end by an Illumina HiSeq 4000 ...
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bioRxiv - Cell Biology 2023Quote: ... Six hundred pg of pre-amplified cDNAs were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit v2 (Illumina #RS-122-2001 and #RS-122-2002) and sequenced on NextSeq 500 using paired-end sequencing (2 x 75) ...
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bioRxiv - Genomics 2023Quote: ... The filtered nuclei were pelleted at 500 r.c.f for 5 min in a pre-chilled fixed-angle centrifuge and then resuspended in 25 μl of 1.25x Tagment DNA Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). For cell cultures ...
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bioRxiv - Developmental Biology 2023Quote: ... We amplified cDNA using a modified mcSCRB-seq protocol and produced libraries using the Illumina DNA Prep kit (Illumina, Inc, San Diego, CA) (112 ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of RNA was used as starting material for library construction with TruSeq RNA Sample Prep Kit V2 (Illumina, RS-122-2002) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... A paired-end genomic libraries with an insert size of 470 bp was prepared using the Genomic DNA Sample Prep kit (Illumina, San Diego, CA) and sequenced on an Illumina NextSeq 1000 platform ...
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bioRxiv - Physiology 2023Quote: ... Each donor library pool was sequenced twice using the Illumina NextSeq500 with NextSeq 500/550 High Output v2 or v2.5 75-cycle kits (Illumina, USA; FC-404-2005). Libraries were sequenced using the following cycle parameters ...