Labshake search
Citations for Illumina :
9301 - 9350 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: RNA samples were submitted to the Cornell Institute for Biotechnology Genomics Center for 3’ mRNA-seq library construction using a QuantSeq FWD kit (Lexogen, Vienna, Austria) followed by 1×75 bp sequencing on an Illumina NextSeq500 (Illumina, Hayward, CA, USA). Sequencing statistics were calculated with v0.15.0 seqkit stats with parameters -j 10 -T(Shen et al. ...
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bioRxiv - Plant Biology 2024Quote: ... A Qubit 2.0 Fluorometer was used to calculate RNA concentration and 4 mg of total RNA was used to prepare libraries with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, US). Single-end sequencing (50bp ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Microbiology 2024Quote: ... and amplicon sequencing were performed using 300-bp paired-end sequencing on the MiSeq Reagent Kit v3 (Illumina Inc., San Diego, CA, USA) and Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was used for poly-A selection of RNA and library construction using TruSeq stranded mRNA LT Kit (Illumina, RS-122-2102). Samples were barcoded and run on a HiSeq 4000 in PE50 to generate 40-50 M reads ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing was performed using the NextSeq 500/550 High-Output v2.5 (150 cycle) Kit (#20024907) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). PhiX Control v3 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing of the metagenomic DNA was performed at the Genomics Core at the University of Tennessee using the NovaSeq 6000 platform (NovaSeq 6000 SP reagent kit, Illumina, San Diego, CA) to generate metagenomic datasets with 2 ξ 250 bp read length (Table S2).
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bioRxiv - Microbiology 2024Quote: ... Paired-end sequencing was carried out on an Illumina MiniSeq system according to the manufacturer’s instructions using a 300-cycle Mid or High kit (Illumina, San Diego, CA, USA), depending on the number of samples in each pool.
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bioRxiv - Microbiology 2024Quote: Whole-genome sequencing was performed on each sample using Illumina Technology (MiSeq and HiSeq 2500) and Nextera XT library preparation kits as instructed by the manufacturer (Illumina, San Diego, CA). Fastq files (raw sequencing data ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated by PCR amplification using specific adaptors according to the manufacturer’s recommendations (NovoNGS CUT&Tag 4.0 High-Sensitivity Kit for Illumina B box, Novoprotein, Suzhou, China). CUT&Tag libraries were sequenced using the Illumina Novaseq 6000 platform (Beijing Novogene Technology Co. ...
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bioRxiv - Plant Biology 2024Quote: ... RNA samples with a RIN value ranging from 6.2 to 8.6 were subjected to sequencing library preparation using TruSeq Stranded mRNA Low Throughput (LT) Sample Preparation Kit (Illumina, San Diego, CA, USA). The prepared library was sequenced using NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... library was generated for 10 plasma exosome samples (n=5, young and old individuals each) using a propriety Illumina Truseq™ Small RNA Preparation kit (Illumina, # RS-930), according to the manufacturers’ guide ...
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bioRxiv - Genomics 2024Quote: ... Genomic sequencing library was prepared from 1 ng of purified DNA using the Illumina Nextera XT library preparation kit (Illumina #FC-131-1096), following the manufacturer’s instructions or with a reduced volume of reagents to 20% ...
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bioRxiv - Genetics 2024Quote: ... nuclei were counted by trypan blue staining and 50,000 nuclei were then recruited for Tn5 transposase reaction by using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, California, San Diego, USA). For head kidney samples ...
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bioRxiv - Microbiology 2020Quote: ... Proteomics and Bioinformatics Laboratory at the University of Hawai‘i – Mānoa sequenced the libraries in two runs using separate 300-bp paired-end Illumina MiSeq v3 reagent kits (Illumina Inc., San Diego, CA, USA). Sequence data are available at the Sequence Read Archive as PRJNA543421.
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bioRxiv - Synthetic Biology 2021Quote: ... genomic library was prepared from four strains (B0, B1, B2 and B3) using the Nextera XT Library Preparation Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Stranded mRNA-seq libraries were prepared at EMBL Genecore facility from 200ng of total RNA using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries from herbarium samples were prepared with 22–157 ng of gDNA using Illumina TruSeq Nano DNA LT Sample Prep kit (Illumina, San Diego, CA, USA). They were sequenced at the GenoToul-GeT-PlaGE platform (Toulouse ...
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bioRxiv - Cell Biology 2020Quote: ... Single-end sequencing was performed on an Illumina HiSeq2000 instrument using the TruSeq SBS Kit HS-v3 (50-cycle) (Illumina, San Diego, CA, US).
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were constructed using 1ng of input DNA in a Low Input, Transposase Enabled (LITE) pipeline developed at the Earlham Institute (Norwich, UK) and based on the Illumina Nextera kits (Illumina, San Diego, CA, USA) [48] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation for Illumina sequencing was performed at the Transcriptome and Genome Analysis Laboratory Goettingen (TAL) using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). Pooled libraries were run on an Illumina HiSeq 4000 to produce 50bp single end reads ...
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bioRxiv - Genomics 2020Quote: ... The remaining 1μl re-dissolved contents were tagmented using 0.6 μl TD Tagmentation buffer and 0.3 μl ATM Tagmentation enzyme from Nextera XT DNA Library Prep Kit (Illumina, catalog no. FC-121-1030) for 5min at 55 °C ...
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bioRxiv - Genomics 2020Quote: ... Individual libraries for each of the analyzed bovines (N=16) were processed using the TruSeq Stranded mRNA Preparation kit (Illumina, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: RNA-seq Expression Analysis: RNA-seq libraries were prepared from total RNA using the TruSeq Stranded mRNA kit (Illumina, Inc., San Diego, CA, USA). Library size distribution was validated using an Agilent 4200 TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: RNA-Seq library preparations were carried out from 500ng to 2000ng of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as antisense RNA) ...
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bioRxiv - Molecular Biology 2021Quote: ... newly synthesized RNA using TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA, Cat# RS-122-2301) according to the Illumina protocol with the following modifications ...
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bioRxiv - Neuroscience 2021Quote: ... One hundred pg of amplified cDNA was tagmented and indexed for sequencing using a Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA). LabChip GX Touch HT High Sensitivity assay (PerkinElmer ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were sequenced using the Illumina NextSeq 500/550 platform with the High Output v2 kit (150 cycles) on paired-end mode (Illumina Inc, San Diego, CA). BCL files were converted to FASTQ and demultiplexed using the bcl2fastq conversion software (Illumina ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA were pooled in equimolar ratio according to sex and Pool-seq libraries were generated using the Truseq nano DNA sample prep kit (Illumina, ref. FC-121-4001) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: RNA libraries from all samples were prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... a library with insert size of 270bp was constructed using TrueLib DNA Library Rapid Prep Kit for Illumina (Genetimes Technology Inc., Shanghai, China) and sequenced using Illumina HiSeq 4000 system (Illumina, San Diego, CA, USA). All raw reads were processed using a local perl script (developed by Biomarker Technologies Corporation ...
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bioRxiv - Genomics 2020Quote: Globin reduction and cDNA library preparation for total RNA was performed with the Illumina TruSeq Stranded Total RNA with Ribo-Zero Globin kit (Illumina, Inc., San Diego, CA). Library quality control included quantification with picogreen ...
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bioRxiv - Genomics 2020Quote: ... according to the instructions for qChIP-Seq and paired-end sequencing (2×75nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s instructions and paired-end sequencing (2 × 75 bp) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Cancer Biology 2019Quote: ... To this end shotgun libraries were generated from 5-10ng of plasma DNA using the TruSeq DNA Nano Sample Preparation Kit (Illumina, San Diego, CA, USA) as previously described[35] ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA-seq libraries were generated from 200-300 ng total RNA with the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Extracted single cell RNA from identified neurons from the STG was used to generate cDNA libraries using TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, California, USA). Libraries were sequenced in a paired-end 150 bp (2×150bp ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Transcriptome libraries were then constructed using poly-A selection with the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, Illumina, San Diego, CA, USA). In brief ...
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bioRxiv - Microbiology 2019Quote: ... 5 μg of bacterial RNA was treated for ribosomal RNA (rRNA) removal by using a Ribo-Zero rRNA Removal Kit (Mouse and Bacteria) (Illumina Inc., San Diego, CA). Directional RNA-seq libraries were created using TruSeq RNA Sample Prep Kit v2 (Illumina Inc.) ...
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bioRxiv - Genetics 2019Quote: ... ribosomal RNA depletion and cDNA library prep was performed using the TruSeq Stranded Total RNA with Ribo-Zero Globin kit (Illumina, Inc., San Diego, CA). The Illumina HiSeq 2500 was used to generate 75 bp reads ...
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bioRxiv - Genomics 2020Quote: ... was prepared from total RNA from a testicular tissue sample of one BSW bull homozygous for the mutant T-allele at Chr6:58373887 using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The library was sequenced using an Illumina NovaSeq6000 instrument ...
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bioRxiv - Cancer Biology 2019Quote: ... Libraries of small RNA from LLC-Exo and CT26-Exo were constructed using TruSeq Small RNA Library Preparation Kits (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we added four mate pair libraries (3 kb, 5 kb, 7 kb, 8 kb) prepared using a Nextera Mate Pair Library Prep kit (Illumina, San Diego, CA, USA). Each library was sequenced on an Illumina HiSeq 2500 sequencer by Novogene (Sacramento ...
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bioRxiv - Genomics 2019Quote: ... while that extracted from fragmented total RNA samples was used as input for the Ribozero reaction (Illumina Ribo-Zero Gold rRNA Removal Kit-Yeast) to remove rRNAs ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... Libraries were sequenced at the UCI Genomics High-throughput Facility on an Illumina MiSeq using the 500-cycle v2 reagent kit (Illumina Cat # MS-102-2003).
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bioRxiv - Genomics 2019Quote: ... and then incubated with 5 μl of Tn5 Transposase of TTE Mix V50 in 45 μL of 1 x TTBL buffer (TruePrep® DNA Library Prep kit V2 for Illumina®, Vazyme, China) at 37°C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... DNA sequencing libraries were generated using Diagenode MicroPLEX v2 kit and single ended reads of 50bp length were generated on an Illumina HiSeq 2500 (Illumina Inc., San Diego, USA). The second experimental replicate was prepared using the NEBNext Ultra II kit (New England Biolabs ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were equimolar pooled and sequenced by the University of Wisconsin – Madison Biotechnology Center with the MiSeq 2×250 v2 kit (Illumina, San Diego, CA, USA) using custom sequencing primers.
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bioRxiv - Genetics 2021Quote: Tagmentation plates were prepared by the combination of 420 uL of 1400 nuclei/uL solution with 540 uL 2X TD Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). From this mixture ...