Labshake search
Citations for Illumina :
801 - 850 of 863 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Next the amplicons were checked for their size and purity on a 1.5 % agarose gel and if suitable subjected to the index PCR using the Nextera XT Index Kit v2 Set C/D (Illumina, FC-131-2003). After index PCR the samples were cleaned again with AmPure XP Beads (Beckman Coulter Life Science Cat ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries for short- and long-read sequencing were generated with the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA) and the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries with an average size of 1.5-2 kb were tagmented and indexed during a second PCR amplification step with the Illumina Nextera XT DNA preparation kit (Illumina, FC-131-1024). Tagmentation was performed according to the manufacturer’s protocol with an input of 500 pg cDNA and amplicon tagment mix for 5 min at 55 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Two micrograms of MDA-amplified DNA were used to generate genome libraries using a TruSeq DNA PCR-Free Kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced using a Rapid-Run on an Illumina HiSeq 2500 to obtain 100 bp paired-end reads ...
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bioRxiv - Genomics 2021Quote: ... The PCR products (4 nM from every single cell) were treated with the Miseq Reagent Kit v2 50 Cycles (Illumina KK, Tokyo, Japan) and sequenced by the MiSeq sequencer (26 bp ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of MDA-amplified DNA were used to generate genome libraries using a TruSeq DNA PCR-Free Kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced using a Rapid-Run on an Illumina HiSeq 2500 to obtain 100 bp paired-end reads ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products (4 nM from every sample) were treated with the MiSeq Reagent Kit v2 (50 cycle format; Illumina KK, Tokyo, Japan) and sequenced using the MiSeq Sequencer (26 bp ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Neuroscience 2022Quote: ... at the HIHG or The American Genome Center at Uniformed Services University of the Health Sciences (USUHS) using the TruSeq DNA PCR-Free library preparation kit and sequencing on the Novaseq 6000 (Illumina, San Diego, CA). WGS was analyzed through a bioinformatics pipeline including alignment to the GRCh38 genome build with BWA mem ...
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bioRxiv - Genomics 2022Quote: ... Libraries were created from DNA of the same HzStark_Cry1AcR male using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit with TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA) to yield a standard paired end library with a 350±50 bp insert size ...
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bioRxiv - Microbiology 2022Quote: ... China) for PCR amplification of the V4 region of the 16S rRNA gene and sequencing using an Illumina NovaSeq (Illumina, San Diego, CA) in two sequencing runs ...
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bioRxiv - Zoology 2022Quote: ... Twelve 150 bp paired-end libraries (six for the Mamm01 primer set and six for the MiFish-U primer set) were prepared with an Illumina TruSeq DNA PCR-free LT Sample Prep kit (Illumina, San Diego, California), spiked with 8% phiX ...
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bioRxiv - Immunology 2024Quote: ... Sequencing of the PCR amplicons spanning the MHC loci was performed on the Illumina MiSeq platform using a v2 500-cycle kit (Illumina, San Diego, CA) in the FHCC Genomics Core Facility or the UCI-Fred Hutch Cancer Centre Laboratory.
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Genomics 2023Quote: ... The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030).
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries of individual DNA samples were prepared using the Illumina TruSeq DNA PCR-free LP kit (Illumina, San Diego, California, United States) in combination with the IDT for Illumina TruSeq DNA UD indexes kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation is performed with or without amplification (Nextera® DNA Flex Library Prep kit or TruSeq® DNA PCR-Free kit, ILLUMINA®), either through automation (MICROLAB Star® ...
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bioRxiv - Cell Biology 2023Quote: ... Editing frequencies in samples collected following transplantation were assessed via deep sequencing of PCR amplified libraries of the CD33 gRNA target region (Table S1) on an Illumina Miseq (Illumina, San Diego, CA) via 300bp paired end reads and a median sequencing depth of 100,000 reads/gRNA target site ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2 μg (mismatch library) of input DNA was used for library PCRs and sequenced on an Illumina NextSeq 2000 system (200 cycles, Illumina sequencing kit #20046812). Sequencing data for the activity library was demultiplexed using our previously published custom script 16 while sequencing data for the mismatch library was demultiplexed with a new custom script (see Supplemental Code) ...
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bioRxiv - Synthetic Biology 2024Quote: ... we performed 12 cycles of pre-amplification PCR and 12 cycles of index PCR and used 0.002 ul TDE1 Tn5 enzyme per cell (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits; Illumina, San Diego, CA, USA). Sequencing was performed using an SE100 sequencing kit (MGI) ...
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bioRxiv - Genomics 2020Quote: ... fragment libraries (average fragment sizes of ∼560 bp) were constructed using TruSeq DNA PCR-Free LT Library Prep Kit (Illumina, San Diego, CA, USA). Paired-end sequences of 150 bp long were determined using HiSeq X (Illumina) ...
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bioRxiv - Genomics 2020Quote: A total of 2 μg of intact genomic DNA was fragmented and used to construct short-insert PCR-free libraries (300 and 500 bp) following the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA). The DNA libraries were used in paired-end sequencing (∼265 million paired-end reads ...
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bioRxiv - Microbiology 2021Quote: ... An average 350-bp paired-end libraries were prepared from extracted genomic DNA by TruSeq DNA PCR-Free High Throughput Library Prep Kit (Illumina, San Diego, CA, USA). Pair-end sequencing (2× 150-bp ...
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bioRxiv - Plant Biology 2021Quote: Libraries for PCR-free short read sequencing were prepared from MN106-Ref genomic DNA using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, San Diego, CA, USA) in combination with TruSeq DNA Single Indexes Set A (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... All the libraries were prepared following the two-step PCR Illumina protocol and these were subsequently sequenced on an Illumina MiSeq instrument (Illumina, San Diego, CA, USA) using 2×251 paired-end reads for the MICROWINE samples and 2×301 paired-end reads for the Biome Makers samples.
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bioRxiv - Genomics 2021Quote: ... Eight whole genome sequencing libraries with a median insert size of 550 bp were constructed using the Illumina TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR free Library Prep Kit (Illumina, San Diego, CA, USA) and size selected at about 400 bp ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We confirmed all strains had marker-less deletions by PCR followed by Sanger sequencing (primers given in Table S1) and Next Generation Sequencing (Illumina HiSeq PE150, >30x coverage). We used P1 transduction to transfer modifying enzyme (ME ...
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bioRxiv - Pathology 2020Quote: The V3-V4 hypervariable regions of the 16S_rDNA were amplified by two steps PCR using v1 primers (Vaiomer) and sequenced using MiSeq Reagent Kit v3 (2×300 bp Paired-End Reads, Illumina, San Diego, CA, USA) as previously described [24] ...
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bioRxiv - Genomics 2021Quote: ... libraries with an average insert size of 450 bp were prepared by the University of Utah’s Huntsman Cancer Institute High-Throughput Genomics Core Facility using the Illumina TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, California, USA). 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...
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bioRxiv - Immunology 2022Quote: ... variable regions V3 to V4 of the bacterial 16S ribosomal RNA gene were amplified by limited cycle PCR and subjected to the Illumina 16S metagenomic sequencing library preparation workflow according to the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The obtained DNA libraries were quantified ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...
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bioRxiv - Plant Biology 2022Quote: Pair-end library preparation for MiSeq sequencing was conducted using the two-step tailed PCR method described on Illumina (Illumina, San Diego, CA, USA). We used the following primer pairs to amplify partial sequences of the 16S rRNA gene:
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... A second short-run PCR was performed in order to ligate the Illumina i7 and i5 barcodes (Nextera XT, Illumina, San Diego, CA, USA), and amplicons were purified again as above ...
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bioRxiv - Molecular Biology 2022Quote: ... A second PCR to insert Illumina adaptors (no further library preparation required) and amplicon sequencing was performed at Genewiz (Illumina-based Amplicon-EZ service).
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bioRxiv - Plant Biology 2023Quote: ... as per manufacturer’s instructions except that reaction volumes were scaled by one-half and 12 PCR cycles were used for library amplification (Illumina, San Diego, California, USA). Paired-end sequencing (2 × 150 bp ...
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bioRxiv - Microbiology 2023Quote: The influenza genome was amplified from RNA extracted from virus isolates using a multi-segment RT-PCR with universal primers (39, 40) and sequenced using Nextera DNA library preparation and MiSeq (PE300) sequencer (Illumina, San Diego, CA, USA), as previously described (13) ...
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bioRxiv - Genomics 2023Quote: ... by the University of Wisconsin-Madison Biotechnology Center (Madison, WI, USA) as specified in the TruSeq DNA PCR-Free Reference Guide (Illumina, San Diego, CA, USA) [17] ...
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bioRxiv - Microbiology 2024Quote: ... the V4 region within the 16S rRNA gene of bacterial DNA was amplified by PCR and sequenced using Illumina MiSeq platform (Illumina, San Diego, CA, USA). Raw sequences were processed following the Divisive amplicon denoising algorithm 2 (DADA2 ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated by PCR amplification using specific adaptors (NovoNGS CUT&Tag 4.0 High-Sensitivity Kit for Illumina B box, Novoprotein, Suzhou, China). The CUT&Tag libraries were sequenced using the Illumina Novaseq 6000 platform (Beijing Novogene Technology Co. ...
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bioRxiv - Genetics 2024Quote: ... A second round of PCR was performed using indexed primer sets to construct sequencing libraries using a Nextra XT Index Kit (Illumina, San Diego, CA, USA). The final libraries were purified using Ampure XP beads and sequenced on an Illumina MiSeq system in 150 × 150 paired-end format ...
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bioRxiv - Genetics 2021Quote: ... A second PCR step was run to add the index primers for sequencing (NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 and 2). PCR products were purified using SPRIselect beads (Beckman ...
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bioRxiv - Genomics 2020Quote: ... After A-tailing and adaptor ligation fragments that carry adaptors on both ends were enriched by 8 cycles of PCR (Illumina TruSeq Nano DNA Sample Kit). The final library was quantified using PicoGreen (Quant-iT ...
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bioRxiv - Microbiology 2021Quote: ... Libraries for shotgun metagenomic sequencing were prepared using a PCR-free method at Novogene (Nanjing, China) on a HiSeq instrument (Illumina Inc. San Diego, CA, USA) with 150 bp paired-end reads and 6 Gb data/sample ...
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bioRxiv - Immunology 2020Quote: ... before fragmenting the samples to 450bp fragments and processing with the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA). Over the three-year duration of the sequencing phase of the project ...
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bioRxiv - Microbiology 2021Quote: ... and gDNA mocks were included in each PCR plate and sequenced alongside the samples sequenced with an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, US) following manufacturer’s recommendations.