Labshake search
Citations for Illumina :
551 - 600 of 863 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... PCR-enriched junctions were deep-sequenced using next-generation sequencing (NGS) technologies (Illumina MiSeq v3 single-end 150 cycles) (Figure S5).
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bioRxiv - Evolutionary Biology 2023Quote: ... and libraries were prepared using the Illumina Truseq DNA PCR-Free Sample preparation kit (Illumina Inc., San Diego, CA). Six pools were combined into a Miseq run (PE250 ...
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bioRxiv - Immunology 2024Quote: ... We then searched for (TRA or TRB) PCR targets isolated using Illumina MiSeq sequencing (sequencing platform keyword: “Illumina MiSeq”); on manual inspection ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified from a 1% agarose gel and sequenced using a NextSeq 550 or NovaSeq (Illumina) to obtain 79+79-nt or 150+150-nt ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation was carried out using the TruSeq DNA PCR-free Sample Preparation Kit (Illumina Inc., San Diego, USA), followed by library quantification using Qubit and q-PCR ...
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bioRxiv - Genomics 2024Quote: ... 0.3fmol of indexed tagged library were sequenced with 14 PCR cycles before quantifying and sequencing on a NovaSeq6000 (Illumina) with 150bp PE reads to give a median 0.9x duplex coverage ...
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bioRxiv - Microbiology 2024Quote: ... libraries were generated manually following the manufacturer’s protocol for Illumina TruSeq DNA PCR-Free Kit (Illumina, San Diego, USA). Briefly ...
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bioRxiv - Genomics 2024Quote: Genome shotgun sequencing was also performed at Canada’s Michael Smith Genome Sciences Centre using the TruSeq DNA PCR-free library prep kit (Illumina) automated via a Microlab NIMBUS liquid handling robot (Hamilton Robotics) ...
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bioRxiv - Neuroscience 2024Quote: ... Final products were purified using a PCR purification kit (Enzynomics, Korea) and sequenced using a MiniSeq sequencer (Illumina, USA). NGS data were analyzed using the program as previously described (53) ...
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bioRxiv - Genomics 2024Quote: ... Library construction was then performed using the TruSeq DNA PCR-Free HT sample prep kit (Illumina, San Diego, CA), followed by sequencing on a HiSeq 2500 system ...
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bioRxiv - Genomics 2024Quote: ... 40.4 M short reads were generated from an Illumina DNA PCR-Free library (Illumina, Inc. for research use only) prepared according to manufacturer’s instructions and Megahit v1.2.9 was used for de novo assembly ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA libraries of 450bp insert size were prepared using the TruSeq DNA PCR-free Library Preparation Kit (Illumina) according to manufacturer guidelines and sequenced to at least 30x coverage by the New York Genome Center (NYGC) ...
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bioRxiv - Cancer Biology 2024Quote: ... 19086) and amplified using 2.5 µl of Universal PCR primer (NEBNext Multiplex Oligos for Illumina, NEB, cat. no. E6861A), 2.5 µl of indexed primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Genetics 2021Quote: Tagmentation plates were prepared by the combination of 420 uL of 1400 nuclei/uL solution with 540 uL 2X TD Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). From this mixture ...
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bioRxiv - Plant Biology 2020Quote: ... to an average size of 500-bp and indexing with sequencing adapters using the TruSeq DNA (PCR free) kit (Illumina). Sequencing was performed on a single lane of an Illumina HiSeq2500 with 500 cycles in high-output mode ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were purified by gel extraction and the concentrations of each sample was adjusted to 25 ng/µl for the 2nd stage barcoding PCR using TG Nextera XT Index Kit v2 Set A (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Peptides carried by the MCRs from sorted cells were amplified from cDNA by RT-PCR using the peptide flanking regions and sequenced on a miniSeq (Illumina). Sequences from the Illumina output files were trimmed ...
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bioRxiv - Immunology 2021Quote: ... The ligated DNA was then barcoded and amplified by a limited cycle PCR and the barcoded Illumina libraries were sequenced by using paired-end 150-base protocol on a NextSeq 2000 (Illumina). Demultiplexed sequence reads were analyzed in the CLC Genomics Workbench v.21.0.3 by (i ...
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bioRxiv - Genetics 2021Quote: ... RNA-seq libraries were prepared from 500ng of RNA samples using an oligo dT based method with 15 cycles of PCR amplification with the Illumina TruSeq mRNA stranded kit (Illumina) and sequenced on the Illumina HiSeq 2000 v4 (50-bp single end read) ...
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bioRxiv - Developmental Biology 2021Quote: ... Input material ranged from 500pg to 15ng of immunoprecipitated DNA and processed according to the kit guidelines (NEBNext End Prep, Adaptor Ligation, PCR enrichment of Adaptor-Ligated DNA using NEBNext Multiplex Oligos for Illumina). Clean up and size selection were performed with AMPure beads (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... The barcode sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824). Sequencing libraries were loaded at 18 pM on an Illumina HiSeq2500 using the following read length ...
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bioRxiv - Genomics 2020Quote: ... Successful amplicons were screened on a 1.5% agarose gel and further purified using Agencourt AMPure XP PCR Purification kit before proceeding to Nextera XT DNA Library Preparation (Illumina), according to manufacture instructions ...
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bioRxiv - Genomics 2020Quote: ... DNA samples were processed using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) on the Hamilton Microlab Star (Hamilton Robotics ...
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bioRxiv - Immunology 2021Quote: ... and amplified for 12 cycles using the SI-PCR forward primer (10x Genomics) and a Nextera i7 reverse primer (Illumina). For the HTO-containing fraction ...
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bioRxiv - Genomics 2020Quote: ... Paired-end libraries were prepared by the Purdue Genomics Core Facility using the Truseq DNA PCR-Free Sample Preparation Kit (Illumina). Whole-genome sequencing was performed using Illumina short-read technology (2×100bp ...
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bioRxiv - Synthetic Biology 2020Quote: ... Adaptors were added to these PCR products and then the pooled libraries were subjected to 300 cycle single-end sequencing using MiSEQ (Illumina). Data was analyzed using Geneious 9.0.5 NGS analysis tools.
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bioRxiv - Genomics 2021Quote: ... Following immunoprecipitation with Dynabeads Protein G beads (invitrogen) in PCR tubes, samples were subject to Tagmentation (5 µl Tagmentation Buffer, 1 µl Tagmentation DNA Enzyme (Illumina), 19 µl Nuclease free water ...
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bioRxiv - Genomics 2021Quote: ... libraries with an insert size of 350 bp with the TruSeq DNA PCR-free gel-free library preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... resequencing of genomic DNA was performed for accessions of ‘Kirakiraboshi,’ ‘Frau Yoshimi,’ ‘Posy Bouquet Grace,’ and ‘Blue Picotee Manaslu.’ Sequencing libraries (insert size of 500 bp) for the four lines were constructed with TruSeq DNA PCR-Free Library Prep Kit (Illumina) to sequence on a HiSeqX (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNA sequences were recovered by genomic PCR analysis and deep sequencing using MiSeq for single-end 150-bp read length (Illumina). The primer sequences used for cloning and sequencing are listed in Supplementary Table S1 ...
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bioRxiv - Systems Biology 2020Quote: ... 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina). The final libraries were purified by AmPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product mixture was sent to FASMAC to acquire 2 × 250 bp paired-end sequences using the MiSeq platform (Illumina).
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bioRxiv - Microbiology 2021Quote: ... High-throughput pyrosequencing of the PCR products was performed using the Illumina MiSeq platform (Illumina, Inc., San Diego, CA, USA). Paired-end reads of the original DNA fragments were excluded from the analysis if they did not well-match a 12-base Golay barcode (one or no errors) ...
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were prepared using 700 ng of DNA and the TruSeq DNA PCR-Free Library Prep Kit (Illumina Inc.) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of gDNA with a DNA integrity number (DIN) of <=6 was used for library preparation using the TruSeq PCR-free library preparation kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the sgRNA coding sequences integrated in the genomic DNA from the initial and 3-times selected populations were amplified by touch-down PCR and sequenced by Illumina deep sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The 9 genomic DNA pools for sequencing were prepared by mixing equimolar concentrations of DNA from which 9 libraries were prepared with an insert size of 350 bp using a TruSeq PCR-free sample prep kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... In the second method purified PCR amplicons (100 ng) were used to prepare sequencing libraries by TruSeq Nano DNA LT Library Preparation Kit (Illumina) with the following modifications ...
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... The final libraries were generated through size selection and PCR enrichment and sequenced as 2×150bp on a HiSeq2500 Sequencer (Illumina). Samples were sequenced to an approximate depth of 35–40 million reads per sample ...
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bioRxiv - Genomics 2022Quote: ... The PCR products were purified with QIAquick Gel Extraction Kit and Qiagen MinElute PCR purification kits and were then sequenced on a Hiseq4000 (Illumina) with 150-bp paired-end sequencing.
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bioRxiv - Biophysics 2022Quote: ... The extracted DNA was then amplified by PCR and attached to barcode oligonucleotides compatible with the MiSeq sequencing platform (Illumina, index1 ...
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bioRxiv - Genomics 2022Quote: ... A library was prepared using a TruSeq DNA PCR-Free library preparation kit according to manufacturer guidance and was sequenced on a NextSeq500 instrument (Illumina) with a NextSeq High Output v2 300 cycle kit ...
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bioRxiv - Microbiology 2022Quote: ... The PCR-generated amplicon libraries were subjected to paired-end 2×250 Illumina MiSeq sequencing (Illumina, San Diego, CA, USA).
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bioRxiv - Neuroscience 2022Quote: ... and finally converted into DNA libraries using custom rpi primers (RNA PCR Primer Index) adapted from the Truseq Small RNA kit (Illumina)106 ...
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bioRxiv - Genomics 2022Quote: ... A paired-end library was prepared with the TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, #FC-121-3001) from 1 µg of the genomic DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... and amplified for 12 cycles using the SI-PCR forward primer (10x Genomics) and a Nextera i7 reverse primer (Illumina). For the HTO-containing fraction ...
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bioRxiv - Developmental Biology 2022Quote: ... Illumina sequencing libraries were prepared from the PCR-amplified cDNA from mutant lungs and controls using the Nextra DNA library prep kit (Illumina). Libraries were sequenced on a NovaSeq 6000 S Prime flowcell (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... the ADT fraction was amplified for 10 cycles with SI-PCR oligo (10x Genomics) and TruSeq Small RNA RPI-x (Illumina) primers to index the samples ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared for each product of long PCR (ranging from 15 to 33 kb) using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina MiSeq platform to generate paired-end (PE ...