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Citations for GE Life Sciences :
4501 - 4550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0 and menin was purified by S200 16/600 (GE healthcare) equilibrated with 50 mM Tris-base ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were eluted with 500 mM imidazole followed by buffer exchange to buffer (50mM Tris, 300 mM NaCl, pH 8.0) with HiTrap 26/10 Desalting column (GE Healthcare). To obtain protein without sumo tag ...
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bioRxiv - Biochemistry 2023Quote: ... Hitag-sumo were purified by using S75-16/600 (GE healthcare) column equilibrated with 50 mM Tris-base ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting supernatant was loaded onto a 1 mL HisTrap FF column (GE Healthcare), pre-equilibrated in Buffer A ...
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bioRxiv - Bioengineering 2023Quote: ... We then pooled all oligonucleotides and coupled the set to Cy3 (GE Healthcare), Alexa Fluor 594 (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto PVDF membranes (Amersham Hybond P – GE Healthcare). Phosphorylated histone H2A (gamma-H2A) ...
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bioRxiv - Cell Biology 2023Quote: Gel-filtration chromatography was performed with the AKTA purifier system (GE Healthcare, Chicago, IL, USA) controlled by the Unicorn 5.1 software ...
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bioRxiv - Biochemistry 2023Quote: Recombined cells expressing either GFP-WT Parkin or GFP-R42P Parkin variant were seeded into a 96-well plate and imaged the next day using the InCell2200 cell imaging system (GE Healthcare). Fluorescence microscopy was performed using the excitation and emission filter settings of DAPI (excitation ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography steps were done in Fast Protein Liquid Chromatography systems (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Purification of His-tagged domains was achieved by nickel-affinity using purification buffer supplemented with 1 mM DTT and gel filtration chromatography in 50mM ammonium bicarbonate pH 7 using a Superdex 200 Increase 10/300 column (GE Healthcare) following published protocols67 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was transferred to an Amersham™ Hybond™ P0.45 polyvinylidene difluoride (PVDF) membrane (GE Healthcare) in 1x Tris/Glycine transfer buffer (National diagnostics ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated before separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Activity of fractions containing partially purified rHAChE was measured using Ellman’s reagent (DTNB ...
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bioRxiv - Biochemistry 2023Quote: ... The protein monomers were separated from oligomers by size exclusion chromatography on a Superdex 200 column (120 mL, GE Healthcare) equilibrated with buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The liberated affinity-tag and the His-tagged SENP1 and 3C proteases were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75pg ...
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bioRxiv - Biochemistry 2023Quote: ... and subjected to hydrophobic interaction chromatography (Butyl Sepharose HP, GE Healthcare) following a second size exclusion chromatography run in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein elutes in the flow through and is subsequently subjected to size exclusion chromatography using a Superdex 200 (GE Healthcare) in a buffer containing 50 mM HEPES (pH 8.2) ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein in the supernatant was diluted to 1% Triton and incubate with Glutathione Sepharose beads (GE Healthcare) in a batch mode ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... with MWCO 30K cut off and loaded onto a Superdex 200 10/300 column (GE Healthcare) in 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... 0.1% DDM using PD-10 Desalting column (GE Healthcare). The desalted protein was immediately mixed with 40-fold excess of the spin label ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed 5 more times and the signal was detected by chemiluminescence using ECL Prime (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... Purified full-length and MTases underwent an extra step of size exclusion chromatography on a gel filtration column (HiLoad® 16/600 Superdex® 200 pg, GE Healthcare) against 20 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed in TBST and detected with anti-Mouse IgG-Horseradish peroxidase (GE Healthcare Life Science, Cat# NA931, 1:5000). For streptavidin staining ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... protein was purified using cell-free culture supernatant by StrepTactin resin (IBA) and by size-exclusion chromatography using Superdex 200 (RBD and NTD) or Superose 6 (S-2P) column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Neuroscience 2023Quote: ... 16μL of PBS-washed protein A agarose beads (GE Healthcare) were added per immunoprecipitation ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) using a Superdex Increase 75 10/300 GL column (GE Healthcare) on ÄKTAxpress (GE Healthcare ...
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bioRxiv - Genetics 2023Quote: ... using a HiPrep Desalting column (GE Healthcare). The N-terminal purification tags were cleaved by overnight incubation with TEV protease ...
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bioRxiv - Biophysics 2023Quote: ... PD-10 columns (GE Healthcare) were used for buffer exchange to 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... in ImageQuant LAS 4000 imager (GE Healthcare Life Sciences). Blots were quantified using ImageJ 1.52 k.
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bioRxiv - Cancer Biology 2023Quote: ... while the latter in RPMI-1640 (GE healthcare, Memphis, TN, USA). Both were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... coli lysates by immobilized metal affinity chromatography (IMAC) using a BioRad NGC FPLC equipped with a His-trap FF Ni2+ − NTA column (GE Healthcare). Columns were regenerated fresh with nickel prior to purification of each sample ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Biochemistry 2023Quote: ... The beads were then resuspended in 650 µL of dialysis buffer and 100 U of Thrombin (GE Healthcare, #GE27-0846-01), to a final volume of 1 mL ...
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bioRxiv - Biochemistry 2023Quote: ... All gels were visualized on a Typhoon FLA 9500 imaging system (GE Healthcare, Chicago, IL) and quantified using Quantity One software (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... followed by size-exclusion chromatography purification using a Superdex 200 column (GE Healthcare) equilibrated in buffer containing 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing purified proteins were concentrated and further purified by a Superdex 200 size-exclusion column (GE Healthcare) equilibrated in buffer containing in the 25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was loaded on a HisTrapTM FF column (GE Healthcare Life Sciences) equilibrated with 5 column volumes (CV ...
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bioRxiv - Biochemistry 2023Quote: ... and GBP2FLK51A were analyzed by size exclusion chromatography using a Superdex 200 increase 10/300 GL column (GE Healthcare/Cytiva). 20 µM protein was incubated at 4 °C overnight with 200 µM of the corresponding nucleotide ...
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bioRxiv - Cell Biology 2023Quote: ... Images were collected using a DeltaVision DV3 widefield microscope and processed using Softworx (both Applied Precision, Issaquah, WA). Images are presented as maximal intensity projections using Omero software ...
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bioRxiv - Biophysics 2023Quote: ... The resulting gel is scanned at 100 µm resolution using a Typhoon FLA 9500 laser scanner from GE Healthcare. Following folding ...
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bioRxiv - Neuroscience 2023Quote: ... and imaged with ImageQuant™ LAS 4000 (GE Healthcare, Chicago, IL, USA). The antibodies used in this study were ...
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bioRxiv - Neuroscience 2023Quote: ... Under real-time ultrasound guidance (LOGIQ P9, GE Healthcare, Chicago, IL, USA), the target nerve was impaled with an insulated tungsten recording electrode (FHC Inc. ...
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bioRxiv - Immunology 2023Quote: ... Superose 6 Increase 10/300 GL (GE Healthcare) in a mobile phase of PBS containing 300 parts per million of sodium azide and at a flow rate of 0.75 mL/min (Dionex Ultimate 3000 pump ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were resuspended in 10 ml of 40% Percoll (GE Healthcare, Chicago, IL) and centrifuged again at 950 x g for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered through a GD/X 0.7 μm filter (GE Healthcare Life Sciences) and loaded onto 2 mL Nuvia IMAC Ni-charged resin (Bio-Rad Laboratories) ...