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Citations for GE Life Sciences :
4751 - 4800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were detected using ECL Western Blotting Detection Reagent (GE Healthcare: #95038-562). Immunoblot band densitometry and normalization to GAPDH expression was performed using NIH ImageJ software35.
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 4°C at 10,000 g for 30 minutes and the supernatants were used to purify target proteins by Ni NTA agarose (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) which had been equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... each sample was buffer exchanged a PD-10 desalting column (GE Healthcare) that had been equilibrated with RIPA buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Uncoupled dye was removed using a PD-10 gel filtration column (GE Healthcare). Fluorescence polarization measurements were recorded on a BMGLabTech CLARIOstar plate reader and analysed using GraphPad Prism (version 6.07) ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Bioengineering 2023Quote: ... Nitrocellulose membranes (8 μm) were from Cytiva (formerly GE Healthcare Life Sciences, UK) and silicon metal powder (3 μm ...
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bioRxiv - Bioengineering 2023Quote: ... Omnipaque 300 contrast agent (GE Healthcare, Chicago, IL) was then injected via the catheter ...
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bioRxiv - Bioengineering 2023Quote: ... A Biosep 10L acoustic filter (Applikon Inc., Foster City, CA) related to the bioreactor thanks to two peristaltic pumps (P-1, GE Healthcare), one for medium recirculation and one for sample withdrawal (Henry et al. ...
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bioRxiv - Genomics 2023Quote: ... and SoftWorx software (Applied Precision) using 1 μm z-stacks ...
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bioRxiv - Plant Biology 2023Quote: ... and their fluorescence was recorded by direct scanning of the gel using an image analyser (Typhoon 9600, GE Healthcare Bio-Science, Chicago, IL) as described (Tasaki et al ...
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bioRxiv - Molecular Biology 2023Quote: ... monocyte or a macrophage were isolated directly from human whole blood using Ficoll-Paque (Premium, GE Healthcare, Chicago, IL, USA) density gradient centrifugation ...
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bioRxiv - Biophysics 2023Quote: Both mFE- and tFE-labeled proteins were further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells were isolated from buffy coats by density centrifugation using Ficoll-Paque Plus (GE Healthcare). CD14+ monocytes were subsequently isolated using magnetic-activated cell sorting (Miltenyi Biotech ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing recombinant protein were combined and purified by gel filtration on a S200 26/60 column (GE Healthcare) equilibrated with standard TBS ...
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bioRxiv - Immunology 2023Quote: ... The NS2B-NS3pro constructs were then additionally purified by gel filtration on an S200 26/60 column (GE Healthcare) equilibrated with TBS ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Neuroscience 2023Quote: ... purified using CL-4B sepharose (GE Healthcare), and injected into pronuclei of C57BL/6J zygotes ...
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bioRxiv - Cell Biology 2023Quote: ... D) were deconvolved using Softworx (Applied Precision; Rača, Slowakia). Images acquired from LSM 710 confocal microscope were deconvolved using Huygens Software (Scientific Volume Imaging ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare), 0.5 μg solids/μl in water was added ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of gadodiamide (Omniscan, GE Healthcare, UK) was administered via the tail vein ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted for 80 min (350 mA, room temperature) onto Amersham Protran 0.2 μm nitrocellulose membranes (GE Healthcare). Membranes were blocked in 5% milk in TBS-Tween for at least 2 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... nylon membrane (GE Healthcare) at 65°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by visualization using an Amersham Imager 680 system (GE Healthcare, MA, USA). The level of β-actin was used as a loading control.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 15% FBS (GE Life Sciences-Hyclone), 0.00072% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... SEC-eluted samples were subject to buffer exchange on Fast-Desalt FPLC column HiPrep 26/10 (GE Healthcare) in 50mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Capturing of ORF1p-His6 protein was performed using Immobilized-Metal Affinity Chromatography (IMAC) by loading of 7M Urea Extract on 5 mL HisTrap FF FPLC column (GE Healthcare) at 0.5 mL/minute flow rate followed by washing with 10 column volumes (CV ...
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bioRxiv - Cancer Biology 2023Quote: ... HiLoad 16/600 Superdex-200 FPLC column (GE Healthcare) was equilibrated with 50mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... sheep anti-mouse IgG-HRP (GE Healthcare, Chicago, IL; catalog # NA931V) was used at 1/5,000.
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bioRxiv - Cell Biology 2023Quote: ... The resulting western-blots were developed using an ECL Prime Kit (GE Healthcare) and detected with an ImageQuant 800 (Amersham).
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bioRxiv - Cell Biology 2023Quote: ... pH=7.5 with a HiPrep column (GE Healthcare). Under native conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Bands were visualized with Enhanced Chemifluorescence substrate (ECF) (GE Healthcare) in the chemifluorescence imaging (ChemiDoc Imaging System ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved by electrophoresis on 10 or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare). Total protein labeling was performed using No Stain Labeling Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then loaded onto a HiLoad Superdex 75 16/60 size exclusion chromatography column (GE Healthcare) that had been pre-equilibrated in Hepes 30 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoblot visualization was performed with the Amersham™ Imager 600 (GE Healthcare, Uppsala, Sweden) following chemiluminescent detection (SuperSignal® West Pico PLUS and West Femto Chemiluminescent Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Canonical secondary antibodies used were sheep anti-mouse or donkey anti-rabbit HRP (GE Healthcare). Mouse TrueBlot® ULTRA ...
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bioRxiv - Cell Biology 2023Quote: ... IMAC purification was performed on an ÄKTA Purifier FPLC system (GE Healthcare) and 20 fractions were eluted in imidazole gradient (20 mM ÷ 500 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Protease inhibitors were either from GE Healthcare (cOmplete) or Thermo Scientific (Halt− protease inhibitor cocktail) ...
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bioRxiv - Cell Biology 2023Quote: SPR experiments were performed at 25°C using Biacore 3000 (GE healthcare) or Biacore T200 (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: The purity of the proteins and their aggregate formation or lack thereof were assessed using a Superdex™ S200 10/300GL SEC column (Cytiva, Vancouver, Canada) and an ÄKTA FPLC™ system (GE Healthcare) in HBS-EP buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... and purified with a G25 column (GE healthcare). Probes were hybridized in Oligo Hybridization Buffer overnight at 42 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (Amersham Hybond ECL; GE Healthcare, RPN303D, Merck, Darmstadt, Germany). After blocking in 5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... or Amersham ECL prime Western blotting detection reagent (GE Healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... and finally purified with Microspin G-50 columns (GE Healthcare). 0.25 mg (Western blot ...