Labshake search
Citations for GE Life Sciences :
4401 - 4450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... After centrifugation supernatant was rotated with 300 – 600 µl Ni sepharose 6 fast flow (GE Healthcare) at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
-
bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was released from the GST beads by thrombin cleavage and purified by MonoQ (GE Healthcare) ion-exchange chromatography ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... concentrated and injected into Superdex-75 column (GE Healthcare) to elute with a final buffer containing 50 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysates were loaded onto a 5 mL NiNTA HisTrap column (GE Healthcare). The column was then washed with 25 mL of wash buffer (4 M guanidine hydrochloride ...
-
bioRxiv - Biochemistry 2023Quote: ... Octamers were purified through size exclusion chromatography Superdex 200 column (GE healthcare) in refolding buffer (10 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... Assembled nucleosomes were purified through a Resource Q ion-exchange column (GE Healthcare). Finally ...
-
bioRxiv - Biochemistry 2023Quote: ... Hiload 16/600 Superdex 200 pg column and AKTA Pure (GE Healthcare) were used for further purification ...
-
bioRxiv - Biochemistry 2023Quote: ... MEMO1 was additionally purified by size exclusion chromatography on a Superdex 75 10/300 GL Increase column (GE Life Sciences) in a buffer containing 50 mM HEPES-Na ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein precipitates were analyzed by SDS-PAGE and transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences). Monoclonal mouse anti-GFP antibodies were used (Cat#11814460001 ...
-
bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
-
bioRxiv - Biophysics 2023Quote: Ion exchange chromatography (IEC) was performed using an ÄKTA Purifier protein purification system controlled by the software Unicorn 3.10 (GE Healthcare, Sweden). CIMmultus QA 1 mL column (BIA Separations ...
-
bioRxiv - Biochemistry 2023Quote: ... plate 2 - 1.6 μg/μL carboxylate beads (1:1 mixture of hydrophilic and hydrophobic beads [mass/mass], GE Healthcare Cat ...
-
bioRxiv - Biochemistry 2023Quote: ... The eluate was buffer exchanged and further purified by gel filtration on Superdex-75 (GE Healthcare) with a buffer containing 50 mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: ... immunoblots were visualized using enhanced chemiluminescence (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... reducing agent was removed by PD-10 desalting column (GE healthcare) exchange to Tris buffer at a final protein concentration of approx ...
-
bioRxiv - Biochemistry 2023Quote: ... The flow-through was collected and purified by ion-exchange chromatography on a HiTrap Q HP column (GE Healthcare Life Sciences, Chicago, IL, USA), using Buffer I1 (20 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... performed on a BIACORE T200 (Cytiva formerly GE Healthcare) as explained in [24] ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated with a 100 kDa cutoff concentrator to 500 μL and loaded on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2023Quote: ... Bound proteins were eluted by boiling in SDS–PAGE sample loading buffer and analyzed by western blot using an anti-GST antibody (GE Healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were further purified on a Superdex 75 10/300 (GE Healthcare) column in 50 mM Hepes pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto a HisTrap HP Ni-NTA column (GE Healthcare, USA) connected to an ÄKTA Pure system (Amersham Bioscience ...
-
bioRxiv - Biochemistry 2023Quote: ... and HiTrap heparin HP column (GE Healthcare) to remove the MBP and bind the protein onto the heparin column ...
-
bioRxiv - Biochemistry 2023Quote: ... the percoll (#17-0891-01, GE healthcare) gradient was prepared using ADS buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... GST-tagged proteins were purified on Glutathione Sepharose resin (GE Healthcare) and eluted with buffer containing 20 mM reduced glutathione ...
-
bioRxiv - Biochemistry 2023Quote: ... 10/60 column (GE Healthcare). The monomeric peak fractions were collected ...
-
bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
-
bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
-
bioRxiv - Biochemistry 2023Quote: ... SPR measurements were performed at 25 °C using a Biacore T200 apparatus (GE Healthcare) and 50 mM HEPES-NaOH pH 7.4 ...
-
bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
-
bioRxiv - Biochemistry 2023Quote: ... or Protein A HRP (GE Healthcare, NA9120V), all at 1:2500 dilutions (Leng ...
-
bioRxiv - Biochemistry 2023Quote: ... The antigen–antibody complexes were pulled down by 30 μl Protein A-Sepharose (GE Healthcare) and specific proteins were detected by the Western blotting analysis ...
-
bioRxiv - Biochemistry 2023Quote: SEC binding assays were performed using a Superdex 200 increase Hiload 10/300 column (GE healthcare) at a flow rate 0.4 ml/min with absorbances monitored at 280 nm ...
-
bioRxiv - Biochemistry 2023Quote: ... Dissociated pMHC I and TAPBPR were isolated by SEC (Superdex 200 Increase 3.2/300, GE Healthcare) in 1×HBS ...
-
bioRxiv - Biochemistry 2023Quote: ... and gel filtered using a Superdex 200 16/60 gel filtration column (GE Healthcare). The peak fractions were concentrated ...
-
bioRxiv - Biochemistry 2023Quote: ... or Superpose 6 10/300 GL (GE Healthcare) size exclusion column ...
-
bioRxiv - Biochemistry 2023Quote: ... and gel filtered using a Superdex 200 16/60 gel filtration column (GE Healthcare). The peak fractions were concentrated ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with glutathione sepharose (GE Healthcare) and then washed with 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... Anti-GST antibody (GE Healthcare) was used for the detection of GST-tagged bromodomains.
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 10 mM glutathione and further purified on a Superdex 200 10/300 (GE Healthcare) column in 50 mM Hepes pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then incubated for 30 minutes at room temperature and excess copper was removed by gel filtration using a PD MidiTrap G-25 desalting column (GE Healthcare; Uppsala, Sweden) equilibrated with acetate buffer (25 mM sodium-acetate ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was then subjected to IMAC purification by loading onto a HisTrap FF column (GE Healthcare) equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
-
bioRxiv - Biochemistry 2023Quote: ... SEC purification was performed on a HiLoad 16/60 Superdex 75 column (GE Healthcare) pre-equilibrated with 25 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... The immunoreaction was visualized with ECL chemiluminescence kit (GE Healthcare Amersham) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96, GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM HEPES ...