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Citations for GE Life Sciences :
4351 - 4400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Membranes were briefly treated with ECL detection reagent (Amersham GE Healthcare) and developed using a Bio-Rad ChemiDoc MP Imaging System ...
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bioRxiv - Biochemistry 2023Quote: Histone proteins were mixed in stoichiometric amounts and eluted through a HiLoad 16/600 Superdex 200pg column (GE Healthcare) to produce H2A-H2B dimers and (H3-H4)2 tetramers separately 40 ...
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bioRxiv - Biochemistry 2023Quote: ... The product was purified using PD-10 columns (GE Healthcare, Chicago, IL) in deionized water containing 3 mM BME ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescent gel images were taken with Typhoon (GE Healthcare) and analyzed with ImageJ for intensities.
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bioRxiv - Bioengineering 2023Quote: ... for HRP was used to detect the signal and the imaging was done using Amersham Imager 600 RGB (GE Healthcare, USA).
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bioRxiv - Bioengineering 2023Quote: ... and the images were captured using Amersham Imager 600 RGB (GE Healthcare, USA).
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bioRxiv - Bioengineering 2023Quote: ... The signals were captured using Amersham Imager 600 RGB (GE Healthcare, USA).
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bioRxiv - Bioengineering 2023Quote: ... and AKTA Flux 6 (GE Healthcare, USA) were used for buffer exchange in this unit process step ...
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bioRxiv - Bioengineering 2023Quote: ... in AKTA pure 25 system (GE Healthcare, USA) followed by washing and elution ...
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bioRxiv - Bioengineering 2023Quote: ... and EPO was captured onto equilibrated AFC column (GE Healthcare, USA) in AKTA pure 25 system (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... FPLC results were evaluated through Unicorn 7.0 software (GE Healthcare, USA) for all chromatography steps ...
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bioRxiv - Cell Biology 2023Quote: All images except for those in Figure 1 were acquired using a DeltaVision Personal DV system (Applied Precision) equipped with a 100X N.A ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant containing GST-hnRNPH2 proteins added to Glutathione Sepharose 4B (GSH; GE Healthcare) beads ...
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bioRxiv - Biophysics 2023Quote: ... and size exclusion chromatography on a Superdex 200 column (GE Healthcare, Uppsala, Sweden). His-tags were removed with TEV protease.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were HRP-linked goat anti-mouse (1:10,000, GE Healthcare) and HRP-linked goat anti-rabbit (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and HRP-linked goat anti-rabbit (1:10,000, GE Healthcare). Antibodies were incubated in 5% non-fat milk PBST (PBS with 0.1% Triton X-100 ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare). For ITC ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... filtered and loaded onto a HisTrap HP column (GE Healthcare). His6-tagged proteins were eluted in elution buffer (lysis buffer with 500 mM imidazole) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The elutions were pooled and concentrated to 500 µl before passing over a PD MiniTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Hence it was injected in MonoQ 4.6/100 PE (GE Healthcare). Buffers used for binding and elution were Buffer A (50 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was loaded on a 5 ml HisTrap HP (GE Healthcare) column ...
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bioRxiv - Biochemistry 2023Quote: ... 200 µL of protein for analytical runs) was injected into the Superdex 75 size exclusion column (GE Healthcare) (volume less than 900 µL) ...
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bioRxiv - Biochemistry 2023Quote: ... and analyzed by a Typhoon 9500 Laser Scanner (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2023Quote: ... Gels were imaged using a Typhoon FLA 9000 (GE Healthcare) to identify fluorescein signals ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was loaded into a HiTrapTm Q HP anion exchange column (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and eluted with ∼ 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ITC experiments were carried out on dA using a high-precision ITC200 microcalorimeter (GE Healthcare, Chicago, IL, USA). Arginine binding was measured at 25 °C in HEPES 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... filtered and loaded into a HisTrapTM HP affinity column (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). The protein was eluted using a 0-500 mM imidazole linear gradient.
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bioRxiv - Biochemistry 2023Quote: ... Image files were saved in 16-bit.gel format and imported into the ImageQuant TL 10.1 software (GE Healthcare). Free NCP signal was quantified in boxes of equal area as relative intensities ...
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bioRxiv - Biochemistry 2023Quote: ... antibodies and the secondary anti-rabbit horseradish peroxidase-linked IgG antibody (NA944V, GE Healthcare for western blotting and 7074S Cell signaling Technology for IHC ...
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bioRxiv - Biochemistry 2023Quote: ... Octyl-Sepharose (C8, from GE Healthcare), geranyl-functionalized carboxymethylcellulose (G-CM) ...
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bioRxiv - Biochemistry 2023Quote: ... and 6xHis-SMT3/SUMO tagged IMPDH2 was initially purified by Ni affinity chromatography using either a HisTrap FF column (GE Healthcare Life Sciences) on an Äkta Start chromatography system or a handpacked HisPur™ Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Expression vector pGEX-6P1 (GE Healthcare) –based recombinant synthesis of CPK21 and CPK23 constructs carrying a C-terminal polyhistidine-tag and N-terminal GST-tag has been described previously (Geiger et al. ...
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bioRxiv - Biochemistry 2023Quote: ... as determined by a calibrated Superdex 75 (GE Healthcare) gel filtration chromatography column (25 mM MES ...
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bioRxiv - Biochemistry 2023Quote: ... His-GCN4-FCT was further purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... clarified culture supernatants were incubated with 15 µL MabSelect resin (Cytiva, formerly GE Healthcare Life Sciences) on a 3 mm orbital shaker at 1000 rpm at room temperature (RT ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with buffer E and concentrated to 10 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) cells and purified by glutathionesepharose 4B beads (GE Healthcare) following previously reported protocols 33 ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a 1 mL HiTrap MabSelect PrismA column (Cytiva, formerly GE Healthcare Life Sciences). Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The quenched reactions were rapidly passed through BA-85 nitrocellulose filters (GE Healthcare, 10402506) and washed with 4 ml of cold wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to Hybond C-Extra nitrocellulose filters (GE Healthcare). Primary antibodies used for immunoblot analysis included ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were filtered through 1.2 μm syringe filters and loaded onto the AKTA Xpress system (GE Healthcare, Fairfield, CO, USA). The purification regime is briefly described as follows ...
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bioRxiv - Biochemistry 2023Quote: ... followed by size exclusion chromatography (Superdex™ 75 pg, GE Healthcare, cat. no. 28-9893-34) into a final buffer of 50 mM TRIS pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... PP1 was purified via a HisTrap™ FF column (GE Healthcare, cat. no. 29048609) followed by size exclusion chromatography (Superdex™ 75 pg ...
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bioRxiv - Biochemistry 2023Quote: ... Phosphorylation was determined by autoradiography and phospho-imaging (Typhoon FLA 9500, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...