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Citations for GE Life Sciences :
4301 - 4350 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The SΔTM protein was then further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) (Fig ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was separated on the 10 % SDS PAGE and transferred on the polyvinylidene difluoride (PVDF) membranes (Hybond™-P; GE Healthcare, Piscataway, NJ, USA) using the wet transfer method (1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Following electrophoresis, protein was transferred onto a 0.45 μm nitrocellulose (Amersham Protran Premium, 10600096) or a PVDF membrane (GE Healthcare Amersham Hybond ECL, RPN68D). 0.4% PFA was used to fix the protein on the membrane ...
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bioRxiv - Biophysics 2021Quote: ... Amplified phage particles at 10-fold dilution were assayed against 20 nM biotinylated membrane proteins in nanodiscs using horse radish peroxidase (HRP)-conjugated anti-M13 monoclonal antibody (GE Healthcare, #27-9421-01). Assays were performed in library sorting buffer (200 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Pathology 2020Quote: ... Equal volumes of plasma were pooled from three mice and lipoproteins were fractionated by fast protein liquid chromatography (ÄKTA pure FPLC system, GE Healthcare, Pittsburgh, PA, USA) and triglyceride and cholesterol in fractions quantified with reagent kits (Wako Diagnostics ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Rv1339 protein was visualized with a FujiFilm LAS-3000 Image Reader and the Amersham™ ECL™ Western Blotting Analysis system (GE Healthcare, UK).
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bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... The Fc and IdeS A were removed using a mix of Protein A Sepharose® Fast Flow (250 μL per 1 mg digested mAb; GE Healthcare Life Sciences) and Ni Sepharose™ 6 Fast Flow (50 μL per 1 mg digested mAb ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were subjected to SDS-PAGE (15 μg per lane) and blotted onto Protran Nitrocellulose Mem-brane-Whatman (GE Healthcare Life Science, USA). Membranes were blocked with Blocking Solution (5% powdered milk in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... A small volume of the bead-coupled GST-fusion proteins was loaded into a ProbeQuant G-50 Micro Column (GE Healthcare, cat no. 28903408) and washed once in ice cold lysis buffer containing 1 % (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was diluted to 100mM NaCl and clarified by centrifugation before application to a 5mL Heparin HiTrap HP (GE Healthcare 17-0407-01) column equilibrated with Buffer B (25mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... aMCFCA was then purified from the supernatant using the AKTA protein purification system through a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02) with loading buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The labelled protein was purified from the excess of free dye by a P10 desalting column with Sephadex G25 matrix (GE Healthcare, Waukesha, WI, USA), divided into aliquots ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before(60) ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Physiology 2023Quote: ... Immunoreactive bands were detected using a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after the membranes were incubated with ECL Prime Western Blotting Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analysis of the immunoreactive bands was performed using the FluorChem FC3 Analysis Software.
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were electrophoresed on an SDS polyacrylamide gel and then transferred to a nitrocellulose membrane (Amersham Protran Premium; GE Healthcare, Waukesha, WI, USA) using a semidry method ...
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bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a Superdex® 200 Increase 10/300 GL on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with PBS (pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Biochemistry 2024Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before[62] ...
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bioRxiv - Biophysics 2024Quote: ... Dyes were incubated with G-actin at a 2-fold molar excess for 2 hours at room temperature before being separated from the labeled protein by applying the labeling reaction to a spin column packed with Sephadex G-50 Fine DNA Grade (GE Healthcare GE17-0573-01) hydrated with A buffer (5 mM Tris-HCL (pH 8) ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were centrifuged at 16,000 x g for 20 minutes and the supernatant used for affinity purification of biotinylated proteins using 25 µl of washed streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301) for 16 hours with rotation at 4℃ ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were centrifuged at 15,000 × g for 15 minutes and the supernatant was used for biotinylated protein capture using 15 μl of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Bioengineering 2021Quote: ... A second purification step was performed for IDO-Gal3 using size exclusion chromatography in an ÄKTA Pure Protein Purification System (GE Life Sciences, Marlborough, MA, USA). Molecular weight and purity were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was further purified by size exclusion chromatography using HiLoad® 16/600 Superdex® 75 pg column (GE Life Sciences, 28-9893-33) equilibrated with 1x Tris buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...