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Citations for GE Life Sciences :
3951 - 4000 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... We eluted bound proteins using an imidazole gradient and subjected them to size-exclusion chromatography (Superdex 200 16/600, GE Healthcare) using 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant recovered after centrifugation was used to purify proteins with GE HisTrap HP column (GE Healthcare Bio-Sciences, Piscataway, NJ) and a Bio-Rad EconoPump (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The sample was centrifuged after incubation with TEV and the supernatant containing the cleaved protein was purified by anion exchange chromatography using a column packed with either Q Sepharose High Performance (GE Healthcare) for Pex13 and 14 IDRs or SP Sepharose High Performance (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions containing the protein complexes were pooled and further purified by size exclusion chromatography using a 16/60 Superdex 200 column (GE Healthcare) equilibrated with a buffer containing 200 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3C protease was removed by Ni-chelating sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The molecular weight of protein complexes was determined after size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted proteins were concentrated and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 400 mM imidazole and concentrated and applied to Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated in 0.2 M sodium bicarbonate buffer (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: A second purification step on Ni-NTA beads was performed to remove any uncleaved protein before to achieve a SEC purification onto a Superdex 75 16/600 GE column (GE Healthcare) in a final buffer containing 10 mM Tris pH = 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the protein were collected and concentrated using 100 kDa cutoff concentrator and loaded onto Superdex 200 10/300 GL column (GE Healthcare) using 20 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... A total of 250 µL of each protein was injected and run on a Superdex 200 Increase (S200) 10/300 column (GE Healthcare) at a flow rate of 0.40 mL/min in SEC-MALS buffer (10 mM Tris pH 8.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were separated on 5–20% SDS‒PAGE gels (Wako) and transferred to Hybond-P membranes (GE Healthcare). The following primary antibodies and dilutions were utilized ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Cell Biology 2024Quote: ... We pooled the α-syn-containing fractions and further purified the protein by size-exclusion chromatography on a HiPrep Sephacryl S-200 HR 16/60 gel filtration column (GE Healthcare) in 50 mM NH4Ac pH 7.4 ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Microbiology 2024Quote: ... Concentrated proteins were further purified by Size-exclusion chromatography (SEC) either using a HiLoad 16/60 Superdex 200 pg column (GE Healthcare) or a HiLoad 16/60 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... These eluted proteins were subsequently passed through a superdex s75 16/60 column attached to an AKTA Pure system (GE healthcare) in 50 mM Tris pH 8.0 and 300 mM NaCl.
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bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
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bioRxiv - Microbiology 2024Quote: ... protein was further purified by size exclusion chromatography on a HiPrepTM 26/60 SephacrylTM S200 HR gel filtration column (GE Healthcare). Protein was eluted isocratically at a flow of 1 ml/min ...
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bioRxiv - Bioengineering 2024Quote: ... SpyTag003-linked non-antigen binding proteins) were injected onto a pre-equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare), or a HiLoad 16/600 Superdex 75 pg column (SUMO-SpyTag003 and affibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten micrograms of protein was separated on SDS-PAGE gels (10% acrylamide) and wet-transferred to a PVDF membrane (GE Healthcare). Membrane blocking was performed with 5% skimmed milk in Tris-buffered saline (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were separated by 15% SDS-PAGE and transferred to 0.2 µm Nitrocellulose Blotting Membrane (#10600001, GE Healthcare Life Sciences) by the semi-dry method (Towbin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against HSP60 rabbit polyclonal (D307 ...
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bioRxiv - Neuroscience 2023Quote: ... 3RCF and 4RCF tau proteins were further purified by FPLC using size exclusion Superdex-75 and Superdex-200 columns (GE Healthcare) in 1 x PNE buffer (25 mM PIPES ...
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bioRxiv - Neuroscience 2023Quote: Purified His-tagged DJ-1 and untagged PGK1 proteins were subjected to further purification using gel filtration with a Superdex200 Increase 10/300 GL size-exclusion column (GE Healthcare) on an AKTA FPLC system (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μg of total protein extracts were fractionated in a 10 % SDS-PAGE followed by a wet transfer to a PVDF membrane (GE Healthcare) [78] ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant loaded to the column and purification was performed by using ÄKTA start protein purification system (GE Healthcare Life Sciences) according to the preferred protocols provided by the purification column manual ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant antibodies were produced in a transient HEK expression system and purified via protein A affinity purification on an automated AKTA system (AKTA explorer FPLC, GE Healthcare). For in vivo studies rabbit V-regions were fused with murine constant of mouse IgG2a heavy chain and kappa chain to minimize immunogenicity ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing the protein of interest were concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (AmershamTM Protran® 0.45 µm, GE Healthcare Chicago, IL, USA). Membranes were blocked for 1 hour with 5% BSA in TTBS (15 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Microbiology 2022Quote: ... Both the 6His- and Strep-tagged proteins were then further purified by SEC on an ÄKTA Purifier using a Superdex 200 16/600 GL column (GE Healthcare), and simultaneously buffer-exchanged into 50 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...