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Citations for GE Life Sciences :
4051 - 4100 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
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bioRxiv - Synthetic Biology 2024Quote: ... and those found to contain the purified protein were pooled and concentrated using a 10 kDa molecular weight cut-off Vivaspin spin column centrifugal concentrator (GE Healthcare) at 3894 x g and 4°C ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Biochemistry 2024Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatants with the GST or GST-AnxA6 fusion protein were incubated with glutathione-Sepharose 4B beads (GE Healthcare Life Sciences) for 2 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... The elutions containing the proteins were loaded onto a HiLoad 16/600 Superdex 75 or HiLoad 16/600 Superdex 200 column (GE Healthcare) pre equilibrated with SEC buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The eluted protein is then applied to a HiLoad Superdex 16/600 200 pg size-exclusion chromatography (SEC) column (GE Healthcare) and run in a buffer containing 25 mM HEPES at pH 8 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated by SDS-PAGE gels (percentage depending on the size of the proteins) and imaged using a Typhoon FLA 7000 PhosphorImager (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... were electrophoresed on 10% sodium dodecyl sulphate-polyacrylamide gel and the separated proteins were transferred onto the PVDF membranes (Amersham International, GE Healthcare). The membranes were blocked with Tris-buffered saline with Tween (TBST ...
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bioRxiv - Microbiology 2024Quote: ... protein samples were subjected to fast protein liquid chromatography (FPLC) using gel filtration on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare). Fractions demonstrating high purity were collected and used in biochemical assays ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by standard SDS-PAGE and transferred to an Immobilon®-P PVDF Membrane (GE Healthcare, Chicago, IL, USA). Blots were blocked overnight at 4°C in blocking buffer (PBS + 0.05 % Tween 20 + 2% Amersham ECL Prime Blocking Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... a 100 μl sample (individual proteins or complexes) was injected into a Superdex 200 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with TBS ...
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bioRxiv - Microbiology 2024Quote: 500 µl of purified proteins from the polymerization assay were loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an AKTA FPLC pure system ...
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bioRxiv - Immunology 2024Quote: ... supernatants underwent protein affinity purification using a high-performance 5 mL HiTrap MabSelect SuRe column (Cytiva, catalog no. 11003494) from GE Healthcare Life Sciences for large-scale production ...
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bioRxiv - Neuroscience 2024Quote: ... The GST-mGluR6 fusion protein was expressed in Escherichia coli strain BL21 and purified with glutathione Sepharose 4B (GE Healthcare, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the excess unreacted dye was removed from each protein by passing it through a 600 μl Sephadex G-25 Fine resin (GE Healthcare), pre-equilibrated with a buffer containing 20 mM Hepes-K pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Microbiology 2024Quote: ... The protein was further purified using size exclusion chromatography with a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) into a final buffer of 20 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein detection and quantification were performed using the SYBR-green fluorescence detection mode of the LAS-4000 image analyzer (GE Healthcare) and Image Quant TL software (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Plant Biology 2024Quote: ... His6-tagged proteins (His6-HsfA2/S-HsfA2/S-HsfA4c/S-HsfB1/S-HsfB2a/DBD/tDBD) and His6 mock proteins were purified using glutathione Sepharose 4B beads (GE Healthcare) and nickel-nitrotriacetic acid (Ni-NTA ...
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bioRxiv - Microbiology 2024Quote: ... Full-length untagged cGAS protein was then ultimately purified using size-exclusion chromatography via a 16/600 Superdex S75 column (GE Healthcare). Purified cGAS was concentrated to ∼10 mg/ml in storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... The molecular weight of protein complexes was determined by size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... glutathione S-transferase (GST) fusion proteins were first purified from soluble extracts by affinity chromatography using Glutathione Sepharose 4B (GE Healthcare). After cleavage of the GST tag with thrombin ...
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bioRxiv - Plant Biology 2024Quote: Purification of ADPG2 and PGLR proteins was conducted using a 1 mL HisTrap excel column (GE Healthcare, Chicago, Illinois, United States). The column was initially equilibrated with 10 volumes of equilibration buffer (50 mM NaP pH 7.2 ...
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bioRxiv - Immunology 2024Quote: ... Human serum samples were heat inactivated at 56°C for 1 h and then incubated with an equal volume of Protein G resin (GE Healthcare) for 3 nights at 4°C on a rocking platform ...
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bioRxiv - Immunology 2024Quote: ... NHP serum samples were heat inactivated at 56°C for 1 h and then incubated with an equal volume of Protein A resin (GE Healthcare) for 3 nights at 4°C on a rocking platform ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated proteins were finally purified by gel filtration chromatography using Hiload 16/60 Superdex 200 prep grade column (GE healthcare) in buffer C and concentrated in the same way as the binary complex ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted sample containing Pol I and Pol III protein fractions was loaded into a 1 mL Resource Q anion exchange column (GE Healthcare), which was then eluted ...
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bioRxiv - Biochemistry 2024Quote: SidF full-length was analyzed with SEC-SAXS mode: the SAXS system was connected to a fast protein liquid chromatography (FPLC) system AKTAGo (GE Healthcare. Samples were loaded onto a Superdex™ 200 Increase 10/300 GL (GE HealthCare ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated using SDS-PAGE in 10% Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 min with PBS 5% milk and incubated with the first antibody at 4 °C overnight with gentle shaking ...
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bioRxiv - Biophysics 2024Quote: ... The eluted scFv16 protein was further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: The size-exclusion chromatography experiments carried out on an ÄKTA Pure Fast Protein Liquid Chromatography (FPLC) system utilizing a Superdex 200 Increase 10/300 column (GE Healthcare). The column was equilibrated with 2 column volumes of PBS running buffer pH 7.4 containing 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Cell Biology 2024Quote: Cell-free extracts from Drosophila heads were incubated with a 50% slurry of equilibrated Protein A-Sepharose beads (GE Healthcare Ltd) in the ratio of 100 µl bead slurry for 1 ml extract for the Pre –clearing step in Immunoprecipitation ...
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bioRxiv - Cell Biology 2024Quote: ... Cellular debris was pelleted by centrifugation for 20 min (18000 × g at 4°C) and GST and MBP fusion proteins were purified using glutathione- Sepharose (GE Healthcare) or amylose (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was separated from MBP using cation-exchange chromatography with a 5 ml SP Sepharose HiTrap column (GE Life Sciences). Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15 ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, PA) using AKTA pure FPLC system ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein separation was carried out with different ion exchange columns: A cation exchange column (HiScreen Capto SP ImpRes, GE Healthcare, München, Germany), an anion exchange column (HiTrap Q HP 1 ml ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... Cleaved protein was separated from TEV and the His-GST tag with a Ni-NTA column and proteins were further purified by size-exclusion chromatography on Superdex 75 16/600 (GE Life Sciences) in NMR buffer (10 mM MES ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...