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Citations for GE Life Sciences :
3901 - 3950 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2021Quote: ... Media was harvested 4 days after transfection and the secreted protein purified using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2021Quote: Samples of purified proteins were thawed on ice and the buffer was replaced using G-25 columns (PD MiniTrap G-25, GE Healthcare) with 25 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... Bound protein was eluted with elution buffer (lysis buffer containing 250 mM imidazole) and loaded onto glutathione Sepharose resin (GE Healthcare) to separate bound NEC from excess His6-SUMO-UL31 ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used to incubate the membrane for an hour followed by protein detection with enhanced chemiluminescence (ECL) western blotting substrate and viewed on Amersham Imager 600 (GE Healthcare). Uncropped immunoblot images are shown in Figure S8.
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bioRxiv - Cell Biology 2021Quote: ... The Trx-6xHis-3C tag was removed by protease 3C cleavage and the untagged protein was further purified by size-exclusion chromatography (Superdex 75, GE Healthcare).
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bioRxiv - Genomics 2021Quote: ... we used the Ascaris proteins cleaved from GST-fusions bound to Glutathione Sepharose 4B by treatment with PreScission protease (GE Healthcare). AsWAGO-2 peptides (YASRRMFELTKQSRDDYRA and RNGGQETSSSSSGEAGHLDI ...
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bioRxiv - Cell Biology 2021Quote: ... was applied and immunoreactive proteins were visualized either using ImageQuant LAS 4000 machine (Raytek) or chemiluminescence film (Scientific Laboratory Supplies or GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Image stacks were deconvolved and protein levels at centrioles were quantified based on maximal intensity projections using Softworx Explorer (GE Healthcare). To quantify pericentriolar protein we applied with Imaris (Bitplane ...
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bioRxiv - Genetics 2020Quote: ... The protein sample was finally purified on size-exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), pre-equilibrated with buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... His6-tagged SECTO-5GS-1TD0 spike protein was extracted from the supernatants using an immobilized Ni SepharoseTM Excel column (GE Healthcare) and eluted with 500 mM Imidazole prior to SEC ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein purification was conducted using a combination of affinity and size-exclusion chromatography (SEC) performed using Äkta Purification systems (GE Healthcare). Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: Further protein purification was achieved by size exclusion chromatography (SEC) through a Superdex™ 200 10/300 GL column (GE Healthcare) using SEC buffer containing 0.5 M NaCl and 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Tev protease and other contaminants were removed with Nickel NTA resin and the unbound protein purified further using a HiLoad 16/60 superdex 75 size exclusion column (GE Healthcare). His-GST-DIDATRX ...
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bioRxiv - Biochemistry 2022Quote: ... To insure the monodispersity of samples all proteins were subjected to SEC directly before collecting of scattering data on a Superdex 200 10/300 GL (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting compounds were purified from cell culture supernatants using an kta Express chromatography system using affinity purification using Mabselect Sure Protein A resin followed by a gel filtration column based on the Superdex200 resin (GE Healthcare). The MabSelect Sure Protein A column was washed with 0.1 M Hepes ...
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bioRxiv - Biophysics 2022Quote: ... Size exclusion chromatography was performed on the concentrated protein using a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer (10 mM Na2HPO4.7H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 25 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein were concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The protein sample was then purified using size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), previously equilibrated with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were cleared by centrifugation and the soluble protein fraction was affinity-purified on a HisTrap Excel column (GE Healthcare). After elution in a 0–300 mM imidazole gradient ...
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bioRxiv - Microbiology 2023Quote: ... and the protein was eluted with an imidazole gradient (300 mM-500 mM) using the AKTA prime chromatography system (GE Healthcare). Fractions containing pure ParR protein (26.5 kDa ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences) pre-equilibrated with buffer F (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed several times in 0.05% TBS-T and proteins were detected using enhanced chemiluminescence (ECL) reagent (GE Healthcare; RPN2106). Antibodies used include ICAM-1 (Cell Signaling Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.4 400 mM Imidazole) The labeled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex-75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove other contaminants and soluble aggregates ...
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bioRxiv - Biophysics 2023Quote: ... the protein was diluted 10 times with PBS buffer and immobilized on a Nickel Sepharose 6 Fast Flow resin (GE Healthcare). The resin was washed extensively with milliQ water followed by PBS buffer pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated and was further purified by a Superdex increase 200 16/600 size exclusion chromatography column (GE Healthcare) that was pre-equilibrated with SEC buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Biophysics 2023Quote: ... Proteins separated based on molecular weight onto the gel were transferred onto a 0.2 μm western blot membrane (GE life sciences) using a thermo scientific transfer cassette for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 35 μg protein from each sample was resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... The protein elutes in the flow through and is subsequently subjected to size exclusion chromatography using a Superdex 200 (GE Healthcare) in a buffer containing 50 mM HEPES (pH 8.2) ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... The PfPHB1-and PfPHB2-GST fusion proteins were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare, USA) using the soluble fractions of the lysed bacterial cultures ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...