Labshake search
Citations for GE Life Sciences :
351 - 400 of 2205 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... a 5 ml HisTrap column (GE Healthcare, UK) was used for immobilized metal affinity chromatography (IMAC) ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented 5% horse serum (GE Healthcare Life Sciences), 1% penicillin/streptomycin (Lonza) ...
-
bioRxiv - Cell Biology 2022Quote: ... using a 5 mL StrepTrap HP (GE Healthcare). The washing buffer contained 100 mM Tris pH 8.0 and 150 mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... 5 ml HisTrap™ HP column (GE Healthcare). For further purification steps the column was connected to a liquid chromatography system (Azura ...
-
bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% membrane blocking agent (GE Healthcare) in TBS and incubated with the corresponding primary antibody ...
-
bioRxiv - Molecular Biology 2022Quote: ... IMAC Column: 5 ml HisTrap HP (GE Healthcare); Gel Filtration Column ...
-
bioRxiv - Molecular Biology 2022Quote: ... loaded onto 5 ml HisTrap column (GE Healthcare), equilibrated in buffer A ...
-
bioRxiv - Microbiology 2019Quote: ... or 5 ml GSTrap HP column (GE Healthcare), depending on the fused tag ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 45 min at 37°C with shaking ...
-
bioRxiv - Microbiology 2021Quote: ... A 5 ml HiTrap desalting column (GE Healthcare) was used to exchange the complex buffer into 100 mM ammonium acetate ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... supplemented with 5% fetal bovine serum (GE Healthcare) and 1% penicillin-streptomycin (Gibco) ...
-
bioRxiv - Cancer Biology 2024Quote: ... using the Amersham 5 AI600 (GE Healthcare, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... The cleavage products were separated by gel electrophoresis on 15% denaturing polyacrylamide gel containing 7.5M urea and 24% formamide and visualized using a Typhoon 9400 Scanner (GE Healthcare Chicago, IL, USA). Activity assays performed with purified Argonaute proteins were carried out with 17 nt long guide G-2 (DNA ...
-
bioRxiv - Plant Biology 2021Quote: ... Radioactive gels were exposed to a BAS phosphor-imaging plate (TR2040, Fuji) for 24 h and visualised using a Typhoon scanner (GE Healthcare, FLA-9500).
-
bioRxiv - Cell Biology 2024Quote: ... This was injected into a size-exclusion column (column size 24 cm x 1 cm) containing Sepharose 4B (84 nm pore size) using an AKTA start system (GE Healthcare Life Science) and eluted with PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.012% LMNG was concentrated up to 8 mg/ml using a 30 kDa MWCO Vivaspin 20 concentrator (GE healthcare). Extensive optimisation of crystal conditions using sitting-drop and hanging-drop vapour diffusion and other post-crystallisation treatments yielded plate shaped crystals after 3-4 days of incubation at 20°C ...
-
bioRxiv - Biochemistry 2020Quote: ... The 100-µl sample (8 mg/ml) was loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with filtered and degassed SEC buffer containing 3% glycerol and 5 mM dithiothreitol instead of MgCl2 and β-mercaptoethanol ...
-
bioRxiv - Biophysics 2021Quote: ... 0.5 M NaCl pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
-
bioRxiv - Biophysics 2022Quote: ... 180 µL of approximately 8 µM sample was injected onto a Superose6 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM TrisHCl pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... VRC293 cells were expanded to grow in suspension in serum-free Expi293 media supplemented with 4 mM L-glutamine through a total of 8 passages via 1.0 L and 3.0 L shake flasks and 25 L Wave bioreactor (GE Healthcare) into a Biostat Cultibag STR 200 Plus (Sartorius Stedim Biotech ...
-
bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Digestive fluids were harvested 7-8 days after treatment and sterile filtrated (Whatman syringe filter 0.2 μm, FP 30/0.2 CA-S, GE Healthcare), then stored at −20°C until further use ...
-
bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
-
bioRxiv - Biochemistry 2019Quote: ... and 5 mg of β-galactosidase G5635-5KU were independently applied to a Superdex 200 10/300 GL analytical gel filtration column (GE Healthcare) equilibrated in 20 mM HEPES KOH ...
-
bioRxiv - Cell Biology 2022Quote: ... The Glo3 BoCCS and GRM regions and β’-COP (residues 300-604) were ligated into BamHI/ NotI sites of pGEX-6P-1 (GE Healthcare), resulting in an N-terminal GST-tagged proteins with a 3C-protease cleavage site ...
-
bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with a radioactive (α-32P dCTP) labeled TRP1 fragment and imaged using a Typhoon FLA 9000 imaging system (GE Healthcare).
-
bioRxiv - Molecular Biology 2019Quote: ... in the presence of 50 µCi of 32P-α-UTP and 1 mM cold UTP and purified on a Sephadex G25 column (GE Healthcare). Hybridization was performed in the modified Church Buffer at 65°C and the membrane was washed two times 5 min in 2X SSC ...
-
bioRxiv - Cell Biology 2021Quote: ... bacterial-expressed GST and GST-α-Taxilin-M (expessed in Escherichia coli strain BL21) were bound to glutathione-Sepharose 4B beads (GE Healthcare) and incubated with bacterial-expressed MBP-γ-Taxilin-M or 293T cell expressed and purified 3×FLAG-CEP170 at 4 °C overnight ...
-
bioRxiv - Bioengineering 2022Quote: The binding affinity of the corresponding Fab versions of the top six affinity matured antibodies as well as the parental clone to α-cobratoxin and Dp7 was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). Antigen immobilization and affinity measurements were performed as previously described8 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... gene specific fragments that were used for VIGS (primer pairs listed in Table S1) were radiolabeled with [α-32P] dCTP (Perkin-Elmer) using the Rediprime II DNA Labeling System (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... which was produced via the 14N(p,α)11C reaction on nitrogen with 2.5% oxygen and 16 MeV protons (GE Healthcare, PETtrace). Briefly ...
-
bioRxiv - Developmental Biology 2021Quote: ... The fragment was amplified with specific primers (see cloning and mutagenesis for the sequence), the PCR product was purified and labelled with α-P32-dCTP (Perkin, NEG5134 250uC) following the manufacturer instructions (GE healthcare, radioactive nucleic acid labeling and detection ...
-
bioRxiv - Molecular Biology 2022Quote: ... The probes (PCR amplified from gDNA or binary plasmid using the oligos in Supplemental Table S3) were internally labelled using [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and hybridisation and washes were performed as described ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
-
bioRxiv - Neuroscience 2021Quote: ... After washing again for 5 x 5 min western blots were visualized with ECL plus Western Blotting detection system (#RPN2231, GE Healthcare) or Immobilon Forte Western HRP substrate (#WBLUF ...
-
bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
-
bioRxiv - Microbiology 2019Quote: ... and was chromatographed sequentially on a 5-ml HiTrap Q HP column and on a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
-
bioRxiv - Biochemistry 2021Quote: ... The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare), which was equilibrated with MonoQ buffer A containing 50 mM NaCl ...
-
bioRxiv - Plant Biology 2020Quote: ... and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
-
bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
-
bioRxiv - Plant Biology 2022Quote: ... at 4 °C overnight and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
-
bioRxiv - Epidemiology 2019Quote: ... measles MV-N was purified by chromatography on a MonoQ HR 5/5 strong anion exchange column (GE Healthcare, Piscataway, NJ) prior to use ...
-
bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
-
bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... and then separated sequentially on a 5-ml HiTrap Q HP column followed by a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...