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Citations for GE Life Sciences :
251 - 300 of 2205 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... the H3 and H4 monomers were purified using a mono-Q HR 5/5 column (GE healthcare) and SP ion exchange resin (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... All the nucleosomal DNA fragments were purified using ion-exchange chromatography (Source Q 5/5, GE Healthcare) and isopropanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in Nap1 chromatography buffer (20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... cDNAs were separated by 8% TBE-urea gels and visualized by Typhoon™ FLA 9000 (GE Healthcare). Sequencing ladders were made using the Sequenase 2.0 DNA Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated using 8-12% polyacrylamide gels and transferred to Amersham Nitrocellulose Membranes (GE healthcare) via Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were separated by 8% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare, Bucks, UK) using the Trans-Blot SD Semi-Dry Transfer system (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... pH 8 supplemented with 0.22 mM DDM by gel filtration using the PD-10 column (GE Healthcare). Samples were concentrated using Amicon®Ultra-4 ultrafiltration device ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were resolved onto an 8% SDS-PAGE and transferred to Protran Premium NC membranes (GE Healthcare). The membranes were blocked with milk (5% [w/v] dried milk in 1X PBST (137mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... and KDM5A F9ΔPID were cleaved from MBP with Factor Xa and further purified using HiTrap SP HP column (GE29-0513-24) on Akta Pure FPLC (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The blots were then exposed onto autoradiography films for 24-48 hours and the signals were detected with the phosphorimager (Fuji, FLA-7000, GE Healthcare). Care was taken to ensure that signals were not over-exposed so that quantitation would be in the linear range ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were then exposed onto autoradiography films for 24-48 hours and the signals were detected with the phosphorimager (Fuji, FLA-7000, GE Healthcare). The signal intensities of the images were measured using ImageJ.
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bioRxiv - Microbiology 2022Quote: ... The plates were scored after 24 and 48 h incubation at 37 °C and imaged using ImageQuant LAS 4000 (GE Healthcare). The last time point (24 h ...
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bioRxiv - Cell Biology 2020Quote: ... were acquired every 10 or 15 minutes for a 24-36 hr period with a 40x oil-immersion 1.3 NA objective using an Olympus DeltaVision Elite microscope (Applied Precision, LLC) equipped with a CoolSNAP HQ2 camera (Roper Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... This protein was purified by the same method as in (24) but with only a single IMAC purification step followed by preparative size exclusion chromatography on Superose-6 Increase (GE Healthcare) in a 16/60 column ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction products were electrophoretically separated on a denaturing 24% polyacrylamide/7 M urea gel and visualized with a phosphorimager (Typhoon 9410; GE Healthcare).
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... The membrane was incubated first with primary antibodies for 24 h at 4°C and then with horseradish peroxidase– conjugated secondary antibodies (GE Healthcare) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood within 24 hours of sample collection using Ficoll-Paque (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
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bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
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bioRxiv - Cell Biology 2020Quote: ... Micrographs in time frames of 10 min were acquired during 24 h in a Deltavision RT imaging system (Olympus IX70/71, Applied Precision) equipped with a Plan apochromatic 20X/1.42 N.A ...
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bioRxiv - Biochemistry 2021Quote: The eluent from the Ni-NTA purification of the expression product of the pET-Duet-1 vector in BL21(DE3)pLysS* cells was loaded onto a 24 ml Superdex-200 10/300 GL (GE Healthcare) column on an AKTA Purifier-10 workstation ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300, bead volume: 24 ml; GE Healthcare), also equilibrated with WB ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the amphipol associated complex was controlled by performing an additional size exclusion step on a Superdex 200 increase column (10/300, bead volume: 24 ml; GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... This was followed by size exclusion chromatography (SEC) using 24 ml Superdex 200 Increase 10/300 GL column on an AKTA-FPLC system (GE Healthcare). Analytical gel filtration was performed in 20 mM Tris pH 7.5 and 150 mM NaCl at the flow rate of 0.5 ml/min.
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bioRxiv - Molecular Biology 2022Quote: ... The blots were then exposed onto autoradiography films for 24-48 hours and the signals were detected with the phosphorimager (Fuji, FLA-7000, GE Healthcare). The signal intensities of the images were measured using ImageJ.
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bioRxiv - Plant Biology 2022Quote: ... oryzae isolates were maintained on Potato Dextrose Agar medium at 24°C and as frozen stocks of dried mycelium on Whatman filter paper (GE Healthcare Whatman™ Qualitative Filter Paper ...
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bioRxiv - Biophysics 2023Quote: Proof-of-concept data were acquired in six healthy volunteers (five female, mean age 30 years, range 24 – 46 years) on a simultaneous 3T SIGNA PET-MR scanner (GE Healthcare); ethics approval was granted by the University of Manchester Research Ethics Committee (reference ...
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bioRxiv - Biochemistry 2023Quote: GB1-FKBP-ICD and GB1-FRB-ICD were mixed at equimolar ratio and loaded onto a 24-mL Superdex 200 column (GE healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: Size-exclusion chromatography coupled with multiple angle light scattering (SEC-MALS) experiments were performed using an S75 column (Superdex 75 10/300 GL 24 ml, GE Healthcare), Agilent HPLC system (Wyatt Dawn HELIOs II ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Cell Biology 2020Quote: ... we used a DeltaVision|OMX microscope version 3 (GE Healthcare) with an objective lens 100X UPlanSApo NA1.40 Oil (Olympus ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~7 mg/mL ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Immunology 2020Quote: ... and protein A-Sepharose (PAS, 3 mg/sample, GE Healthcare), after a preclearing step on PAS (1 h ...
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bioRxiv - Immunology 2020Quote: ... the presence of β-actin was revealed (clone 8H10D10) Development was performed with ECL Western blotting reagents (GE Healthcare, UK).
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bioRxiv - Molecular Biology 2021Quote: ... the immobilized proteins were eluted and further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were assessed by SDS–PAGE followed by Coomassie blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted out and further purified by a mono Q 5/5 column (GE Healthcare) and a Superose S6 Increase 5/150 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed and probed with 100 μl/well of a goat α-GST antibody (GE Healthcare Life Sciences, 27457701V) diluted 1:800 and incubated for one hour at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was then purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). The fractions containing α-Synuclein were dialyzed overnight against water and dried after aliquoting 200 ug into low binding Eppendorf tubes ...
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bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was further purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). Exclusively monomeric α- Synuclein was obtain by size exclusion chromatography using a HiLoad® 26/600 Superdex® 75 prep grade (from GE healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... The secondary antibodies used were a sheep α-mouse antibody conjugated to horseradish peroxidase (HRP) (GE Life sciences, Marlborough MA) at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences ...
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bioRxiv - Microbiology 2019Quote: ... or 5-mL HisTrap column (GE Healthcare), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% newborn calf serum (GE Life Sciences), and 1x penicillin/streptomycin in an atmosphere of 5% CO2 at 37°C ...