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Citations for GE Life Sciences :
551 - 600 of 2205 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM imidazole at pH 8) and loaded on a nickel affinity chromatography column (1 mL HisTrap, GE Healthcare, 17-5247-01). Bound proteins were eluted in elution buffer (20 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2020Quote: ... infected cells were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix as described ...
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Increasing phagocytosis of microglia through targeting CD33 with liposomes displaying glycan ligandsbioRxiv - Immunology 2021Quote: ... the pellet was dissolved in 3 ml of 30% Percoll (Percoll PLUS, GE Healthcare) and carefully layered on top of 70% Percoll and immediately centrifuged (650 rcf for 20 min) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2021Quote: ... followed by concentrating the sample (VivaSpin 6 MWCO 3 kDa cut-off, GE Healthcare) to 8 μM ...
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bioRxiv - Plant Biology 2021Quote: ... 100 mM DTT and 2% v/v IPG buffer pH 3– 10 (GE Healthcare) was applied to the strips ...
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bioRxiv - Biochemistry 2020Quote: ... A volume of 3 mL of Glutathione Sepharose 4B resin (17075605, GE Life Sciences) was added to the soluble fraction and incubated for a minimum of 6 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... chromatin lysates were incubated for 3 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... were pooled and coupled with either Cy®3 Mono NHS Ester (GE healthcare) or Alexa FluorTM (AF ...
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bioRxiv - Microbiology 2023Quote: ... cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on customized microarray chip using commercially available hybridization platform (Agilent ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) which had been equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated for 2–3 minutes with ECL detection reagent (Amersham GE Healthcare), and were then developed using a ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... GST-tagged constructs were cloned into a modified form of pGEX4T-3 (GE Healthcare) with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Genetics 2021Quote: ... Twenty-five ng were labelled with 50 µCi [α- 32P]dCTP (SRP-305; Hartmann Analytic; Braunschweig; Germany) with the Amersham Rediprime II Random Prime Labeling System (RPN1633; GE Healthcare, Chalfont St Giles, UK) and purified on Illustra G-50 ProbeQuant micro columns (GE Healthcare ...
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Neuroscience 2019Quote: Whole-brain T1-weighted MRI data were acquired using a General Electric HDx 3T MRI scanner and a 8-channel receiver head coil (GE Healthcare, Waukesha, WI) at the Cardiff University Brain Research Imaging Centre with an axial 3D fast spoiled gradient recalled sequence (echo time 3 ms ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved products were further purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences). The purity of the protein was inspected using a 15% SDS PAGE (Figure S1) ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... liver and enzyme treated lung were prepared by meshing organs through 70 μm nylon cell strainers in sRPMI-1640 with 8% FBS (HyClone Laboratories, GE Healthcare, Logan, Utah). After red blood cells lysis with NH4Cl (liver ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty micrograms of total RNAs were separated on 15% acrylamide/8 M urea gel and transferred onto an Amersham™ Hybond™-N+ nylon membrane (GE Healthcare). The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... High resolution images of individual PvLS parasites and PHH nuclei were obtained by capturing 8 planes in the Z dimension using a 100×objective on Deltavision Core (GE Healthcare Life Sciences) and deconvoluted using softWoRx (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham, GE Healthcare Life Sciences). The membrane was blocked in 5% skimmed milk (HIMEDIA ...
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bioRxiv - Biochemistry 2021Quote: ... loaded onto a 5-ml HiTrap Heparin HP column (GE Healthcare), and eluted with a 12-CV gradient from buffer A1 to A2 (1.5 M NaCl ...
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bioRxiv - Biophysics 2020Quote: ... using a MonoQ column (5/50 GL, GE Healthcare Life Sciences) equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was loaded onto a 5 ml HisTrap column (GE Healthcare) pre-loaded with Ni2+ ...
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bioRxiv - Molecular Biology 2021Quote: ... and a Superose S6 Increase 5/150 GL column (GE Healthcare) in buffer containing 30 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5% glycerol and loaded onto a Heparin column (GE Healthcare), eluted with a linear NaCl gradient (0.1 to 1M) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by vortexing for 5-10 min and sonication (GE Healthcare) on ice at level 5 for 20 times (3 s pulse 3 s pause) ...
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bioRxiv - Biochemistry 2020Quote: ... eluted though a tandem 5 ml HiTrap desalting column (GE Healthcare) to remove excess cross-linking reagents ...
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bioRxiv - Biochemistry 2019Quote: ... HiTrap DEAE FF and Mono Q 5/50 GL (GE Healthcare). Elution was performed with a 0–1-M NaCl gradient ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Biochemistry 2020Quote: ... protein was loaded onto a 5 mL HisTrap column (GE Healthcare) and eluted with a linear imidazole gradient (20-350 mM ...
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bioRxiv - Biochemistry 2020Quote: ... and applied onto a GE HisTrap HP 5 mL (GE Healthcare). The column was washed with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol using a HiTrap Desalting column (GE Healthcare). All protein samples were aliquoted and stored at −80 °C.
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bioRxiv - Biochemistry 2020Quote: ... Gels were imaged with the Amersham IR Typhoon 5 (GE Healthcare) excitation at 685 nm ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified using 5 mL Ni-NTA column (GE Healthcare) and a HiLoad Superdex200 16/60 size exclusion column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 5% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL), 2 mM L-glutamine (ThermoFisher) ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was loaded to 5 mL HisTrap HP column (GE Healthcare). With rPtxS1 proteins ...
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bioRxiv - Biophysics 2020Quote: ... and desalted (5 ml HiTrap, GE Healthcare Life Science, Chicago, USA). KR2 was incorporated into liposomes with an inside-out orientation ...
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bioRxiv - Biophysics 2020Quote: ... were purified using 5 ml SPFF cation exchange column (GE Healthcare) with a 100 ml gradient of 0.1 to 1 M NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... an environmental chamber maintained at 37° and 5% CO2 (GE Healthcare); and a Plan ApoN 60× 1.42 oil objective ...
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bioRxiv - Biochemistry 2022Quote: ... and then again on a Superdex 200 5/150 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5% fetal bovine serum (HyClone, GE Healthcare, Chicago, IL) and 10 μg/ml gentamicin (MilliporeSigma ...
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bioRxiv - Biochemistry 2020Quote: ... and circulated through a 5 ml HisTrap HP column (GE Healthcare) for 2 h ...
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bioRxiv - Bioengineering 2021Quote: ... and loaded into a HisTrap HP 5 mL column (GE Healthcare) using an ÄKTA pure 25 (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... and on one 5 ml StrepTrap HP column (GE Healthcare, US) equilibrated in binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...