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Citations for GE Life Sciences :
201 - 250 of 2205 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... and subcloned into pGEX-4T-3 (GE Healthcare) using BamHI and SalI ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was diluted to 500 µl with running buffer A (50 mM Tris, pH 9) and loaded on a 1 ml Resource™ Q anion exchange column (GE Healthcare Life Sciences). A linear gradient to 40% running buffer B (50 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... resolved by 8% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare, Munich, Germany) by semi-dry blotting ...
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bioRxiv - Microbiology 2021Quote: ... the dye values were measured using ImageQuant TL version 8 (GE Healthcare, Chicago, IL, USA) and Adobe Photoshop Elements were normalized to density values per mm2 of a worm’s projection area ...
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Rationally designed protein bandpass filters for controlling cellular signaling with chemical inputsbioRxiv - Synthetic Biology 2023Quote: Surface plasmon resonance (SPR) measurements were performed on a Biacore 8 K device (GE Healthcare). The mutual designed binder (LD3 ...
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bioRxiv - Biochemistry 2023Quote: ... 8 μL of the reaction mixture was spot on Amersham Hybond-XL paper (GE healthcare) and dried out ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...
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bioRxiv - Biophysics 2021Quote: ... followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare). Purified Nap1 was snap frozen in buffer containing 10 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2020Quote: ... Analytical size exclusion chromatography was performed at 24 °C using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a mobile phase containing 10 mM HEPES (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision, LLC) equipped with eGFP ...
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bioRxiv - Cell Biology 2021Quote: ... in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision, LLC) equipped with eGFP ...
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bioRxiv - Biochemistry 2022Quote: ... and allowed to adsorb to the gel bridge strip and the 24 cm linear gradient IPG strips (GE Healthcare) by swelling overnight ...
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bioRxiv - Immunology 2020Quote: The stability of FPhtΔ3 under acidic conditions was evaluated by SEC on a 24 mL Superdex200 column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The α-domains of the proteins were purified to homogeneity by size exclusion chromatography (Superdex 75 GE Healthcare) equilibrated in 25 mM HEPES pH 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for western blotting are as follows: α-mouse-HRP (1:1000) (GE Healthcare, Piscataway, NJ) for α-O-GlcNAc primary antibody ...
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bioRxiv - Bioengineering 2023Quote: ... S17 were prepared by blotting tau and α-synuclein proteins onto nitrocellulose membrane (GE Healthcare Life Science, 10600004). Human HT40 fibrils (Stressmarq ...
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bioRxiv - Plant Biology 2023Quote: ... The resultant probe was internally labelled with [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and used for hybridization of the membrane ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing α-syn were collected and further purified by passing through a GSTrap 4B column (GE Healthcare). The purified α-syn was determined to be homogeneous by SDS-PAGE followed by Coomassie blue staining and the concentration was adjusted to 5 mg/mL in PBS (pH 7.4) ...
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bioRxiv - Cell Biology 2024Quote: ... which was probed with a horseradish peroxidase-coupled secondary antibody (donkey-α-rabbit IgG, GE HealthCare, 1:400). Subsequent staining was performed using 3-amino-9-ethylcarbazol ...
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bioRxiv - Molecular Biology 2024Quote: Dried blood spot (DBS) samples were collected on Whatman 3-mm filter paper (Whatmann No. 3, GE Healthcare Life Sciences, PA, USA) from enrolled patients on Day 0 (before treatment ...
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bioRxiv - Biophysics 2020Quote: ... packed in water in a 5 mm inner diameter Tricon 5/100 Column (GE Healthcare, USA). Packing occurred by combined sedimentation and a steady flow of 100 µl/min through the column ...
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bioRxiv - Molecular Biology 2020Quote: ... and subcloned into the pGEX-6P-3 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... 25 mM HEPES/NaOH pH 8) into a 1 mL HiTrap Heparin HP column (GE Healthcare). This column was further washed with buffer NT.A ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were purified by centrifugation and filtration through a Whatman 8 µM nuclepore membrane (GE Healthcare) followed by a second filtration step through a 5 µM nuclepore membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were subsequently imaged for 8 hours on a DeltaVision Elite imaging station (GE Healthcare), equipped with a CoolSNAP HQ2 camera ...
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bioRxiv - Bioengineering 2023Quote: ... cored vertebral specimens were scanned using quantitative micro-CT (MS-8, GE Healthcare, London, Ontario, Canada) with 70 kVp X-ray tube peak potential ...
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bioRxiv - Microbiology 2020Quote: ... GSTrap columns (5 mL; GE Healthcare) were used with an Akta Prime FPLC system (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of HisTrap (GE Healthcare) was used for the affinity purification and Gel filtration was performed on HiLoad 16/60 Superdex 200 (GE Healthcare) ...
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bioRxiv - Physiology 2022Quote: ... including 5% serum (Hyclone, GE Healthcare), 1% penicillin-streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... A final polishing step was performed by size exclusion chromatography (SEC) on a 24 mL Superdex 200 increase (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Dried gels were exposed to a phosphoscreen for 16-24 hours and imaged directly using a Typhoon FLA900 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Approximately 24 hours later cells were fixed and imaged with an INCell Analyzer 2000 or 2200 (GE Healthcare Life Sciences) as described above.
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bioRxiv - Biochemistry 2022Quote: Purified protein or protein complex (>1.5 mg/mL) was subject to a 24 mL Superdex 200 analytical gel filtrating column 10/300 GL (GE healthcare) at a rate of 0.5 mL/min at 20 °C by HPLC (Varian) ...
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bioRxiv - Biophysics 2021Quote: ... ∼900 μL of 100 μM TS-β1AR was applied at 0.3 mL/min to a 24-mL Superdex 200 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2019Quote: ... The HisTrap eluate was subsequently loaded on a Superdex 200 10/300 GL 24 ml gel filtration column (GE Healthcare), and eluted with buffer A plus 250 mM imidazole at a flow rate of 0.75 ml min−1 ...
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bioRxiv - Neuroscience 2022Quote: ... After 24-36 h aliquots were tested for the presence of large protofibrillar aggregates using size-exclusion chromatography (GE Healthcare). Fractions containing less than 20% monomer ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated for 1 hour with secondary α-rabbit antibodies conjugated with horseradish peroxidase (HRP) (GE healthcare, NA934) diluted by 1:10,000 in 5% skim milk in TBST ...
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bioRxiv - Plant Biology 2023Quote: ... The probe was labelled with [α-32P] dCTP (BRIT, India) using the Rediprime random DNA labelling system (GE healthcare). The prehybridization ...
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bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...