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Citations for GE Life Sciences :
3651 - 3700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ITC titration curves were recorded with a ITC200 calorimeter (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... FhuF was then purified from the supernatant using a His-trap affinity column (GE Healthcare) where the fraction containing FhuF was eluted at 20 mM Potassium Phosphate pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatographic steps were carried out on an Aekta Explorer system (GE Healthcare, Freiburg, Germany). To allow selection of binders by ribosome and phage display ...
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bioRxiv - Biochemistry 2023Quote: ... FhuFred was prepared using sodium dithionite and then its excess was removed through buffer exchange in a HiTrap® Desalting Column (GE Healthcare). The different concentrations were used to avoid and deal with the spectra overlap between FhuF and the Fe(III)-siderophores ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting supernatant was loaded onto Q-sepharose beads (GE healthcare) and eluted via a NaCl gradient ...
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bioRxiv - Biochemistry 2023Quote: ... and imaging was performed using the Amersham™ ImageQuant™ 800 (GE Healthcare).
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bioRxiv - Genetics 2023Quote: ... All blots followed by anti-rabbit IgG HRP secondary antibody (1:5000; NA934V, GE Healthcare). TORC1 activity experiments were performed as previously described 71 ...
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bioRxiv - Cell Biology 2023Quote: ... the combined fractions were subjected to anion-exchange chromatography on two connected 5 ml HiTrap Capto Q ImpRes (GE Healthcare Life Sciences) anion-exchange columns using a linear gradient ...
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bioRxiv - Cell Biology 2023Quote: ... Forty 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare) for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Cell Biology 2023Quote: ... slowly filtered through 0.2 µm pore size filter (6876-2502, Whatman®, GE Healthcare Life Sciences)) and added to a polystyrene cuvette (67.741 ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then applied to a HiTrap cobalt-chelating TALON crude column (GE Healthcare Bio-Sciences AB). Enzyme purification was performed according to the manufacturer’s instructions using an Äkta FPLC system ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Fractions were eluted with an imidazole buffer (50 mM NaH2PO4, pH 7.4, 300 mM NaCl, 500 mM imidazole) on an AKTA Prime Plus (GE Healthcare, Piscataway, NJ). Fractions were analyzed by SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant was syringe-filtered to 0.22 µm and then purified using a 5 mL HisTrap FF nickel affinity column (GE Healthcare, Piscataway, NJ). Fractions were eluted with an imidazole buffer (50 mM NaH2PO4 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 0.01% azide by a PD-10 column (GE Healthcare, Piscataway, NJ). Enzyme concentrations were determined by the sample’s 280 nm absorbance ...
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bioRxiv - Genomics 2023Quote: ... or protein-G-sepharose beads (GE Healthcare, 17-0618-01) for 1h at 4˚C ...
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bioRxiv - Genomics 2023Quote: ... Lysates were pre-cleared with 30µl of either protein-A-sepharose (GE Healthcare, 17-5280-01) or protein-G-sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... 40 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Genetics 2023Quote: ... and purity and concentration were determined by a NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). An RNA pool for each biological replicate was generated by mixing the samples extracted from the picked berries ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... with 10% FBS (Hyclone GE Healthcare), and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... GST or GST-importins were allowed to bind to glutathione-Sepharose 4B (GE Healthcare). In a typical experiment 100 μl beads were pre-equilibrated in IP-buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... or the respective negative controls (non-targeting small interfering RNA, siCTR, GE Healthcare Dharmacon) using Lipofectamine RNAiMAX Reagent (Thermo Fisher Scientific #13778-150 ...
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bioRxiv - Cancer Biology 2023Quote: ... loaded onto a HiLoad 26/600 Superdex 75 column (GE Healthcare Biosciences) and eluted with storage buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, Il, USA). T cells were negatively purified with EasySepTM beads (human T-cell isolation kit ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunostained bands were detected by chemiluminescence (GE Healthcare).
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bioRxiv - Developmental Biology 2023Quote: ... and size-exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) in HBS (HEPES-buffered saline ...
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bioRxiv - Cancer Biology 2023Quote: ... Chemiluminescence was detected using ImageQuant™ LAS4000 (GE Healthcare, Chicago, IL).
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bioRxiv - Cancer Biology 2023Quote: ... 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). Rabbit polyclonal anti-SDHC (1:200 ...
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bioRxiv - Biophysics 2023Quote: ... The untagged protein was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase 10/300 column; GE Healthcare) and equilibrated with storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... The pooled fractions were further purified by anion exchange chromatography (MonoQ; GE Healthcare) equilibrated in buffer containing ...
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bioRxiv - Biophysics 2023Quote: ... and loaded onto a gel filtration column (Superdex 200 Increase 10/300 GL, GE Healthcare) on an ÅKTA system (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... concentrated using ultrafiltration (Amicon) and loaded onto a Superose 6 Increase 10/300 gel filtration column (GE Healthcare) equilibrated with 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated at room temperature for 4 hours and was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase column; GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... Upon reaction completion the reaction mixture was separated on a Superdex 75 10/300 GL column (GE Healthcare) at a flow rate of 0.5 mL/min and eluted in PBS buffer to isolate the monomeric fraction and buffer exchange into PBS ...
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bioRxiv - Biophysics 2023Quote: ... The protein solution was passed over two coupled HiTrap Desalting Columns (GE Life Sciences, Pittsburgh, PA) to remove DTT and buffer exchanged into 20 mM Tris (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... or 20mM HEPES at pH 7.4) by PD-10 desalting column (GE Healthcare). To further purify the protein ...
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bioRxiv - Cell Biology 2023Quote: ... followed by amplification with the GenomiPhi Kit (GE Healthcare) according to the manufacturer’ s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was performed using ECL substrate solutions (Pierce) and a Imagequant LAS4000 luminescent image analyzer (GE Healthcare). Membrane scans were analyzed using ImageJ software ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The purified protein was buffer exchanged with PD-10 columns (GE Healthcare) into 100 mM potassium phosphate buffer pH 7.6 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and eluted with an imidazole gradient (10-500 mM) on Äkta FPLC (GE Healthcare). The purified protein was buffer exchanged with PD-10 columns (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... or IgG as a control were immobilized on 50 μl of Protein A–Sepharose 4 Fast Flow beads (GE Healthcare) for 3 h and incubated with clarified lysates.
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bioRxiv - Cell Biology 2023Quote: ... and quantitated using an Amersham Imager 600 (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2023Quote: ... GST tagged VCP/p97 was affinity-purified with a GSTTrap FF column (GE Healthcare) with a flow rate of 1-5 ml/min on an ÄKTA purification system ...
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bioRxiv - Cell Biology 2023Quote: ... and RNAi negative control were purchased from GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µM Drp1 was injected onto a Superose 6 10/300GL column in an ÄKTApure FPLC system (GE Healthcare) connected in line with DAWN Heleos-II 18-angle MALS and Optilab T-rEX differential refractive index (dRI ...