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Citations for GE Life Sciences :
3501 - 3550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... the proteins and domains were purified by ÄKTA Go System (GE Healthcare, USA) with Ni-NTA affinity chromatography column (HisTrap HP ...
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bioRxiv - Microbiology 2023Quote: ... and size exclusion chromatography column (Superdex 200 Increase 10/300 GL, GE Healthcare, USA). Finally ...
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bioRxiv - Microbiology 2023Quote: ... of which 450 µl were subjected to isoelectric focusing on pH 4-7 IPG strips (GE Healthcare), followed by SDS-PAGE with 12 % acrylamide gels ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Plant Biology 2023Quote: ... were obtained by spectrophotometry (OD260/280 and OD260/230 > 1.8) (NanoVue GE Healthcare, Munich, Germany). RNA integrity number (RIN ...
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bioRxiv - Immunology 2023Quote: ... 15 ml Ficoll–Paque Plus (GE Healthcare) was added to SepMate tubes and centrifuged at 1000 g for 1 minute (RT) ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from COVID-19 patients were isolated from the blood using Ficoll (GE Healthcare) density gradient media and transferred into 96-well plates at a concentration of 2.5 × 106 viable cells per ml in 200µl (0.5 × 106 cells per well ...
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bioRxiv - Immunology 2023Quote: ... Isolation was performed by differential density centrifugation using Ficoll-Paque (GE Healthcare). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... Detection of HRP-linked antibodies was performed using chemiluminescence detection kits (ECL™ or ECL Plus™, GE Healthcare) according to the manufacturer’s instructions and visualized using ChemiDoc™ Touch Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2023Quote: ... The PBMCs were obtained from 30 mL peripheral blood by using Ficoll-Paque (GE Healthcare, Uppsala, Sweden). The iNKT ...
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bioRxiv - Biochemistry 2023Quote: ... A HisTrap™ HP column (GE Healthcare Life Sciences, Piscataway, NJ, USA) was equilibrated with equilibrium buffer (8 M Urea ...
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bioRxiv - Biochemistry 2023Quote: ... of GcapOCP2 or CrinOCP2 holoform samples (1 mg/ml in 100 μl) was carried out on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a tandem of consecutive detectors Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... and gel filtration on a Superdex 75 16/600 (GE Healthcare). Electrophoretically homogeneous preparations were obtained ...
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bioRxiv - Bioengineering 2023Quote: Bleaching movies were acquired with a commercial OMX-SR system (GE Healthcare) equipped with a Toptica diode 488 nm and 405 nm lasers and stage/objective heating set to 37 degrees C ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 2 mL Ni-Sepharose High Performance slurry (GE Healthcare) for 1 hour ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... and gels were imaged with a Typhoon FLA9500 scanner (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: The ITC experiments were performed using MicroCal iTC200 (GE Healthcare) to calculate the binding affinity of phenol towards various MopRAB mutants.70 All the protein and ligand (phenol ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... the blots were dried with tissue paper and developed using Amersham ECL Western Blotting Detection system (GE Healthcare). Images were captured on a ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... then ACKR3 was purified by size exclusion chromatography (SEC) using a Superdex 200 Increase (10/300 GL column) connected to an ÄKTA purifier system (GE Healthcare) and eluted in buffer elution buffer without Flag peptide or VUF11207 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluate was then further purified by size exclusion chromatography using a Superdex 200 Increase (10/300 GL column) connected to an ÄKTA purifier system (GE Healthcare) in a buffer containing 20mM Hepes (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences) pre-equilibrated with buffer F (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the desired protein were pooled together and concentrated to 2ml using a 10 kDa cutoff Vivaspin20 concentrator (GE Healthcare Biosciences). Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... RNA was capillary transferred onto a Hybond-N+ nylon membrane (GE Healthcare) and UV-crosslinked in a Stratagene Auto-Crosslinker with 1200 mJ dosage of UV-C ...
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bioRxiv - Microbiology 2023Quote: ... IgG fraction was purified using Protein G Sepharose columns (GE Healthcare) and part of it was labelled with AlexaFluor568 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and exposed to ECL film (GE Healthcare) or luminescence captured with an ImageQuant 800 (Amersham) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and cell pellets were re-suspended in 37% isotonic Percoll (GE Healthcare, IL, USA) at room temperature ...
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bioRxiv - Immunology 2023Quote: PBMCs were isolated by Ficoll-Paque (GE Healthcare, Fairfield, CT) density gradient centrifugation of blood samples at 2200 rpm for 25 minutes with no brake or acceleration ...
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bioRxiv - Immunology 2023Quote: ... Mononuclear cells were isolated by a percoll gradient (70%/37%) centrifugation (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Images were acquired on a Delta Vision microscope (Applied Precision) with a 60×/1.42 Plan Apo N (Oil ...
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bioRxiv - Biophysics 2023Quote: ... The buffer was exchanged for PBS by size exclusion chromatography on PD-10 columns (GE Healthcare). Purification of IgG was verified using SDS-PAGE and Coomassie blue staining ...
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bioRxiv - Biophysics 2023Quote: ... Proteins separated based on molecular weight onto the gel were transferred onto a 0.2 μm western blot membrane (GE life sciences) using a thermo scientific transfer cassette for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... the gels were dried at 50°C for 50 min and exposed to a phosphor screen that was subsequently scanned with a Typhoon™ trio imaging system (GE Healthcare). The results are presented in Figure S3 ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were purified by use of Ficoll Paque (GE Healthcare, Piscataway, NJ, USA)-based density centrifugation ...
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bioRxiv - Immunology 2023Quote: ... the ECL Western blotting detection reagents (RPN2106, GE Healthcare) were used together with a FUSION FX chemiluminescence imaging system.
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bioRxiv - Cell Biology 2023Quote: ... HRP activity was detected using ECL Prime Western Blotting Detection Reagent (Cytiva, Tokyo, Japan) and ImageQuant LAS 500 (GE Healthcare). Levels of β-catenin proteins were normalized to that of GAPDH using ImageJ software (National Institutes of Health ...
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bioRxiv - Cancer Biology 2023Quote: ... which was determined by imaging of DAPI stained cells using the Incell 2200 or 6500 Cell Imaging Systems (GE Healthcare, Parramatta, Australia).
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A) coupled with horseradish peroxidase were from GE Healthcare, an anti-goat IgG secondary antibody was from Santa Cruz.
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... This oligo was conjugated with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1- carboxylate (SMCC linker through NH2-NHS ester reaction. The SMCC-linked oligo was purified through Nap column (GE Healthcare Life Sciences). The purified SMCC-linked oligo was conjugated with OVA through cysteine (thiol)-maleimide group coupling ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were purified with a HiTrap Q HP cation exchange column (GE Healthcare). Further details are available elsewhere (96).To produce unlabeled proteins ...
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified by anion exchange (HiTrap Q, Cytiva) or SEC (Superdex 200 Increase 10/300 GL, Cytiva) chromatography at ÄKTA FPLC (system ÄKTA pure; GE Healthcare). The purity of the protein was checked by the SDS PAGE.
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bioRxiv - Cell Biology 2023Quote: ... Free Flag peptides were removed using a PD-10 Desalting Column (GE Healthcare). The conjugate was further purified by a new PD-10 Desalting Column ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 35 μg protein from each sample was resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Plant Biology 2023Quote: ... Typhoon scanner (GE healthcare) was used to detect the hybridization signal ...
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bioRxiv - Plant Biology 2023Quote: ... The probe was labelled with [α-32P] dCTP (BRIT, India) using the Rediprime random DNA labelling system (GE healthcare). The prehybridization ...
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bioRxiv - Plant Biology 2023Quote: ... Typhoon scanner (GE healthcare) was used to detect the hybridization signal ...
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bioRxiv - Microbiology 2023Quote: ... Trimer was purified on a Superdex 75 Increase 10/300 GL column (GE Healthcare), whereas SApNPs were characterized on a Superose 6 10/300 GL column ...