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Citations for GE Life Sciences :
3751 - 3800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and further purified by affinity chromatography on an affinity column containing the above mentioned connexin36 segment protein coupled to CNBr-activated Sepharose (14 mg to 2.5 g Sepharose 4B; GE Healthcare). The specificity of the affinity purified antibodies was tested on tissue extracts and cryostat sections (12 µm thick ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein was further purified by size exclusion chromatography using a Superdex 200 Increase 100/300 GL column (GE Healthcare). The purified scFv16 were pooled ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 1 h and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... The resultant probe was internally labelled with [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and used for hybridization of the membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AKTA pure 25 system (GE Healthcare, Life sciences). Purified rAAVs were subsequently concentrated using centrifugal concentrators (Amicon Ultra-15 and Ultra-0.5 ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated with SDS-PAGE and subsequently transferred to Amersham Hybond-P PVDF Transfer Membrane (RPN303F, GE Healthcare). Membranes were blocked in 5% milk in Tris-buffered saline with 0.01% Tween 20 and incubated with the antibodies listed in Supplemental Table 4 ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9 dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Superdex 200 increase 10/300 GL column (GE Healthcare #28-9909-44) equilibrated in cas9 GF buffer (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Superose 6 increase 10/300 GL column (GE Healthcare #29-0915-96) equilibrated in ORC GF buffer (25 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were placed into an IN Cell Analyzer 2000 (GE Healthcare) at 37°C for live-cell imaging of uptake every 30 min for a total of 2 h.
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bioRxiv - Molecular Biology 2023Quote: ... Time-lapse microscopy was performed using a DV Elite microscope (GE healthcare) with a sCMOS (PCO ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-mouse/-rabbit-IgG-HRP (Donkey polyclonal, GE Healthcare, Freiburg, DE) were used as secondary antibodies.
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bioRxiv - Biochemistry 2023Quote: ... using a Superdex200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The RNA was then transferred to a HyBond-N+ nylon membrane (GE Life Sciences) using an electrophoretic transfer apparatus (Idea Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... and HiTrap Q HP (GE Healthcare) anion exchange chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were centrifuged in self-forming Percoll® Plus (GE Healthcare, Milan, Italy) gradients according to a previously published method175 ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were developed with the chemiluminescence kit Prime Western Blotting Detection Reagent (GE Healthcare, Milan, Italy) and the signal was acquired with a Molecular Imager ChemiDoc XRS+ (Bio-Rad Laboratories S.r.l. ...
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bioRxiv - Biochemistry 2023Quote: ... the protein solution was concentrated to ∼6 mL using a centricon (MW cutoff = 10 kDa) and was purified via size exclusion chromatography with a HiLoad 26/600 Superdex 75 pg column (GE Healthcare). The purified protein solution was diluted to 135 μM (∼2.0 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... and applied to a 5 mL HisTrap FF column (GE Healthcare) at a rate of 2 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a HiTrap DEAE_FF 5 ml column (GE Healthcare) pre-equilibrated with 0.1 mM ZnCl2/20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: Expressed PSA was purified by anion exchange chromatography using POROS HQ resin (GE Healthcare) with the sample and resin initially equilibrated with 20 mM Tris (pH 7.4) ...
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bioRxiv - Biochemistry 2023Quote: ... and loaded onto a Tricorn MonoS 10/100 GL cation exchange column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were dried and quantified using a Typhoon phosphorimager (GE Healthcare). The intensity of full-length CRISPR variant ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto a HiLoad Ni2+ column (GE healthcare) and the protein eluted using a linear gradient with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was further purified on an S75 10/300 gel filtration column (GE healthcare) equilibrated with 10 mM Tris ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... and the dried blot was exposed to a Storage Phosphor Screen (Amersham) overnight at room temperature and imaged using a Typhoon FLA9500 scanner (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibody ECL anti-mouse IgG (GE Healthcare Life Sciences, Buckinghamshire, UK), diluted 1:5000 in TBS buffer containing 5% milk powder was applied to the membrane for 90 minutes at room-temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved protein was diluted further to 0.2M NaCl concentration and applied to cation exchange 10/10 Mono S column (GE Healthcare). Small terminase was eluted in two peaks with 0.2- 1M NaCl gradient ...
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bioRxiv - Biochemistry 2023Quote: ... Both anti-His and anti-Strep immunoblots were developed using Amersham ECL immunoblotting reagents (GE Healthcare Life Sciences, Buckinghamshire, UK).
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibody ECL anti-mouse IgG (GE Healthcare Life Sciences, Buckinghamshire, UK), diluted 1:5000 in TBST containing 5% milk powder was applied to the membrane for 90 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... columns for protein purification were obtained from Cytiva (formerly GE Healthcare Life Sciences (Uppsala, Sweden)) ...
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bioRxiv - Biochemistry 2023Quote: ... His6-HsCHIP21-154-TPR was purified by Ni2+-affinity chromatography using a 5mL HisTrap HP column (GE Healthcare) loaded in 25 mM HEPES pH 7.5 and 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were wet transferred to nitrocellulose membrane (0.45 μM, GE Healthcare Life Sciences) at 100 V for 75 min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was affinity-purified using Ni-Ni-Sepharose High-Performance Affinity Matrix (GE Healthcare Biosciences) and eluted using 500mM imidazole containing buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by SEC (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... pre-packed PD-10 desalting columns (GE Healthcare Cat. no. 17085101) were used instead of gel-filtration columns ...
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bioRxiv - Biochemistry 2023Quote: ... Phosphorylated proteins were visualized using an FLA7000IP Typhoon Storage Phosphorimager (GE Healthcare Life Sciences) and quantified in ImageJ ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was loaded onto a 1 ml StrepTrap HP column (GE Healthcare Life Sciences, Buckinghamshire, UK) that was equilibrated with Lysis buffer.
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bioRxiv - Biochemistry 2023Quote: ... Twenty units of PreScission Protease (GE Healthcare) were added ...
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bioRxiv - Biochemistry 2023Quote: ... CG6178 was further purified using size-exclusion chromatography with a Superdex 75 column (GE Life Sciences). CG6178 was concentrated to 30 mg/mL using cellulose Millipore Amicon® Ultra – 4 Ultracel – 30K centrifugal filters (MilliporeSigma).
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bioRxiv - Biochemistry 2023Quote: ... Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva) using the running buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... samples were injected in a Superdex 200 10/300 Increase (GE Life Sciences) previously equilibrated in the corresponding buffer ...
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bioRxiv - Bioengineering 2023Quote: ... and the density gradient medium Ficoll-Paque (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM of OCP and 20 μM of FRP were pre-incubated in SEC buffer for 15 min at room temperature and then loaded onto a Superdex 200 Increase 5/150 column (GE Healthcare) pre-equilibrated with the same buffer at 0.45 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... hydrophobic interaction chromatography (HIC) on a 5-ml Phenyl-Sepharose column (GE Healthcare), and gel filtration on a Superdex 75 16/600 (GE Healthcare) ...