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Citations for GE Life Sciences :
3801 - 3850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Protein purification included two steps of immobilized metal-affinity chromatography separated by a thrombin-catalyzed His-tag cleavage and thrombin removal on a heparin column (GE Healthcare), hydrophobic interaction chromatography (HIC ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified with Superose 6 Increase 10/300 column (GE Healthcare) (Fig ...
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bioRxiv - Biochemistry 2023Quote: ... further the collected flowthrough was injected into the Superose 75 Increase 10/300 column (GE Healthcare) (Fig ...
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bioRxiv - Biochemistry 2023Quote: ... Hybond positively charged membrane (GE Healthcare), and gel dryer filter paper (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT) and loaded onto a 5 ml Q FF column (GE Healthcare) at 5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... the fraction was applied to a PD-10 column (GE Healthcare, IL, USA) to exchange the buffer for 50 mM ammonium acetate (pH 6.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) in 20 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ITC titration curves were recorded with a ITC200 calorimeter (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... FhuF was then purified from the supernatant using a His-trap affinity column (GE Healthcare) where the fraction containing FhuF was eluted at 20 mM Potassium Phosphate pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatographic steps were carried out on an Aekta Explorer system (GE Healthcare, Freiburg, Germany). To allow selection of binders by ribosome and phage display ...
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bioRxiv - Biochemistry 2023Quote: ... FhuFred was prepared using sodium dithionite and then its excess was removed through buffer exchange in a HiTrap® Desalting Column (GE Healthcare). The different concentrations were used to avoid and deal with the spectra overlap between FhuF and the Fe(III)-siderophores ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting supernatant was loaded onto Q-sepharose beads (GE healthcare) and eluted via a NaCl gradient ...
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bioRxiv - Biochemistry 2023Quote: ... and imaging was performed using the Amersham™ ImageQuant™ 800 (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... and MWCO 100,000 Da for ABCDE) and subsequently applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare). The eluted protein fractions were further concentrated to 12-30 μM before sample preparation.
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bioRxiv - Biophysics 2023Quote: ... The sample was then loaded to a 5 mL HisTrap HP column (GE Healthcare) and washed with approximately 10–20 column volumes of the binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was then loaded onto a PD-10 Empty column (GE Healthcare) and washed with 2.5 column volumes B20 buffer containing 5 mM β-mercaptoethanol followed by 5-7 column volumes of B30 buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... The immune complex was incubated with protein A-Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were mixed 1:1 with phosphate-buffered saline (PBS) and layered on Ficoll–Paque (GE Healthcare; 1714402). Cells were spun at 800 g for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and then the GST-YTHDF1 and GST were purified with glutathione beads (GE Healthcare). The resulting purified GST-YTHDF1 or GST was incubated with Myc-STUB1 purified from HEK293T cells ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™, GE Healthcare) in plates containing ZYP agar medium (supplemented with 100 µg.mL-1 ampicillin and 34 µg.mL-1 chloramphenicol) ...
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bioRxiv - Biochemistry 2023Quote: Size exclusion chromatography was performed on a Sephadex S75 10/300 column (GE Healthcare), using a 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag was separated from DBD on an S75 10/300 GE gel filtration column (GE healthcare) equilibrated with 10 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... on an ÄKTA prime Plus system (GE Healthcare Biosciences) pre-equilibrated with buffer E ...
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bioRxiv - Biochemistry 2023Quote: ... The HiTrap® Desalting Columns (GE Healthcare Life Sciences, Piscataway, NJ, USA) were used to exchange the buffer ...
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bioRxiv - Immunology 2023Quote: ... IgGs were purified using MAbSelect™ (GE Healthcare) affinity chromatography.
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bioRxiv - Genomics 2023Quote: ... At least two dried blood spot (DBS) samples (50 µL per spot) were collected on Whatman 3MM filter paper (GE Healthcare Life Sciences). DBS samples were placed in plastic bags and stored at 4°C until processing ...
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bioRxiv - Immunology 2023Quote: ... Samples then underwent Percoll (GE Healthcare Life Sciences) density gradient centrifugation to remove myelin contamination ...
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bioRxiv - Microbiology 2023Quote: ... unpurified proteins contained in the supernatant of transfected HEK293F cells were immobilized on 2G12 or PGT145 functionalized CNBr-activated Sepharose 4B column (GE Healthcare) by flowing at 1 mL/min or overnight incubation at 4 °C on a roller bank ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Genetics 2023Quote: ... or SoftWoRx v.7.0.0 (GE Healthcare) and processed with FIJI69.
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bioRxiv - Genetics 2023Quote: ... The 135 images obtained were reconstructed with a Wiener constant of 0.01 using SoftWoRx (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... and gels were visualized using a 473 nm laser and bandpass filter (510-530 nm) on a Typhoon™ FLA 9500 biomolecular imager (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2023Quote: ... Synaptosomes were then stained with CypHer5E (GE Life Sciences, PA15401) via agitation ...
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bioRxiv - Genomics 2023Quote: ... using Dyenamic ET terminators (GE Healthcare) according to the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM DTT.) Cascade was further purified by size exclusion chromatography using a Superose 6 increase column (GE healthcare) in SEC buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain cells isolated were washed and subjected to 30% Percoll (GE Healthcare) density gradient centrifugation at 500g for 15 min ...
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bioRxiv - Neuroscience 2023Quote: Images were acquired with a Delta Vision Spectris compound microscope (Applied Precision/GE Healthcare, Mississauga, Ontario) using a 60x oil immersion objective (NA 1.4) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... in a dot blot apparatus (GE Healthcare). Thereafter ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Plant Biology 2023Quote: The GST-Tip6ΔSP-mCherry and GST-mCherry recombinant proteins were purified using glutathione sepharose 4B beads (GE Healthcare, Cat. No. 17075601) following the product protocol ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was transferred to Hybond-N+ membranes (GE Healthcare, Cat #RPN303B) by the capillary transfer method using blotting paper ...
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bioRxiv - Neuroscience 2023Quote: ... at a flow rate of 0.5 ml/min using an AKTA purifier 10 (GE Healthcare). For each run ...
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bioRxiv - Plant Biology 2023Quote: ... coli (BL21 strain) were performed according to the GST Gene Fusion System Handbook (GE healthcare Life Sciences). Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein bands were visualized by using the Western Blotting Reagents (RPN2106, GE Healthcare) and chemiluminescence was captured using MicroChemi (DNR Bio-Imaging System).
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bioRxiv - Neuroscience 2023Quote: ... Band intensities were quantified using ImageQuant software (GE Healthcare). For some experiments ...
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bioRxiv - Neuroscience 2023Quote: ... with MOPS buffer and transferred overnight at 4°C onto nitrocellulose membranes (GE Healthcare) with Tris-Glycine transfer buffer (25 mM Tris-base ...