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Citations for GE Life Sciences :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... then poured onto loaded onto a pre-equilibrated HiTrap DEAE FF 5 ml column (GE Healthcare) into Buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... PmScsC-EcDsbA was initially purified by immobilized metal affinity chromatography (IMAC) using a 5 mL HisTrap Fast Flow column (GE Healthcare) followed by cleavage of the N-terminal His6-tag with TEV protease and reverse IMAC ...
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bioRxiv - Microbiology 2023Quote: ... Pooled His-A51R factions were run over a Superdex 200 Increase 10/300 size-exclusion chromatography (SEC) column (GE Healthcare), exchanged into Storage Buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... The ribosome-protected fragments were purified using Sephacryl S-400 HR spin columns (GE Healthcare Life Sciences) and RNA Clean & Concentrator-25 kit (Zymo) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1mM EDTA pH 7.7) and transferred onto Amersham Protran Premium 0.45 NC Nitrocellulose blotting membranes (GE Healthcare) using a Bolt Mini-Gel system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... protein bands were detected by using Amersham ECL Select Western blotting detection reagent (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and underlaid with 5 mL of 80% Percoll (GE Healthcare) in a 15 mL tube ...
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bioRxiv - Microbiology 2023Quote: WB was performed on a semi-dry western blot device transferred to an Amersham Protran 0.45 mm nitrocellulose (NC) blotting membrane (GE Healthcare, Chicago, IL, USA) at 52 mA for 45 min ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Amersham Protan, GE Healthcare). Incubation with specific antibodies was followed by peroxidase-conjugated secondary antibodies and detection using SignalFire ECL Reagent (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... dried and exposed to phosphor screens analyzed by Typhoon FLA 7000 (GE Healthcare) or to x-ray films ...
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bioRxiv - Microbiology 2023Quote: ... Band intensities were determined by the ImageQuant TL Software (GE Healthcare Life Sciences). The background signal was subtracted from values used for graphical visualization of quantification.
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated with horseradish peroxidase-linked secondary antibody (GE Healthcare) and visualized using the ECL pico plus chemiluminescence method (Pierce ...
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bioRxiv - Microbiology 2023Quote: ... aMCFCA was then purified from the supernatant using the AKTA protein purification system through a Ni-NTA HisTrap affinity column (GE Healthcare GE17-5248-02) with loading buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Genomics 2023Quote: ... Yeast cells were harvested by filtration with 0.45 µM Whatman cellulose nitrate membrane filters (GE Life Sciences #7184-004). Ribosome profiling was conducted as detailed in (McGlincy and Ingolia ...
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bioRxiv - Genetics 2023Quote: ... ECL Select reagent (GE Healthcare, #RPN2235), and a Vilber Lourmat Fusion Fx7 imager or a ChemiDoc Touch Imaging System (BIORAD).
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bioRxiv - Genetics 2023Quote: ... Antibody binding was detected using the ECL system (GE Healthcare). Images were acquired on G:BOX gel imaging system (Syngene ...
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bioRxiv - Molecular Biology 2023Quote: ... real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) was used to determine the thickness of the VL of the right leg as previously described by our laboratory (18 ...
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bioRxiv - Molecular Biology 2023Quote: ... and GST-G3BP1-ΔIDR3 (deleting E411-Q466) was then loaded on a GST HP column (GE Healthcare) equilibrated with GSTBufferA (50 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were visualized using ECL plus reagent (GE Life Sciences), and the Fiji program was used to quantify protein abundance.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (GE Healthcare Life Sciences), 5% KnockOut Serum Replacement (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: The supernatant or cell extract was then filtered through a 0.45 µm pore filter and loaded on a HisTrap HP column (GE Healthcare) with immobilized Ni2+ beads equilibrated with HisBufferA (50 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... harvested by filtration with 0.45 µM Whatman cellulose nitrate membrane filters (GE Life Sciences #7184-004). 100 µg/mL cycloheximide was added to the culture immediately before filtration ...
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bioRxiv - Genomics 2023Quote: ... Cy3B–NHS ester (GE Healthcare PA63101), or Alexa Fluor 488–NHS ester (Invitrogen A20000 ...
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bioRxiv - Microbiology 2023Quote: ... and filtering through a 0.45-μm syringe filter followed by a 0.2-μm syringe filter (Whatman GE Healthcare Life Sciences, Chicago, IL). Sterile soluble mucin was then added to the fermentation buffer at a final concentration of 0.5%.
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bioRxiv - Neuroscience 2023Quote: ... Cells were purified by a linear 40% Percoll (GE Healthcare) centrifugation step at 650 g without brakes for 25 min and the myelin top layer and supernatant were discarded ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% bovine growth serum (BGS, HyClone Bovine Growth Serum, GE Healthcare Life Sciences) and 100 U of penicillin/streptomycin/ml (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... ImageQuant LAS4000 (GE Healthcare Europe, Freiburg, Germany). Primary antibodies used are as follows ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were resolved by SDS-PAGE and transferred to Amersham Protran membranes (GE Healthcare) in a tankblot device (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy data of intracellular GFP fusion proteins were collected using a DeltaVision microscope system (GE Healthcare Inc., USA) equipped with a pco.edge 4.2 sCMOS camera (PCO ...
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bioRxiv - Immunology 2023Quote: Immune cells were isolated from livers by Percoll (17-08-91, GE Healthcare) separation ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Biophysics 2023Quote: ... a final purification was done with a Superdex 200 size-exclusion chromatography (SEC) column (GE Healthcare, Chicago, Illinois) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The NP without MBP containing buffer was diluted to reach 150 mM NaCl concentration by adding 20 mM NaH2PO4 pH 8 and loaded on a heparin column (GE Healthcare) to remove bound bacterial RNA ...
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bioRxiv - Biophysics 2023Quote: ... Samples were injected on the Äkta prime plus chromatography system (Cytivia) and separated on a Superose 6 10/300 column (GE Healthcare) in SEC buffer (10 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min at 4°C and incubated for 45 min with amylose beads (GE Healthcare). The loaded protein was cleaved on column by GST-tagged 3C protease at 4°C overnight ...
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bioRxiv - Biophysics 2023Quote: ... NP was eluted by an NaCl gradient and concentrated for further purification by size-exclusion chromatography on an SD200 column (GE Healthcare) in 50 mM Tris(HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto a 1 mL HisTrap HP column (GE healthcare) preequilibrated with binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: Chitosan solution synthesized with 0-2000 μM of KM-met were filtered through Puradisc 0.2-μm polyvinylidene fluoride (PVDF) membrane filters (GE Healthcare Life Sciences, Pittsburgh, PA, USA) and measured immediately ...
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bioRxiv - Biophysics 2023Quote: ... clarified lysate obtained from the cells was bound to a HiTrap Chelating HP column (GE Healthcare) in low imidazole buffer (50 mM PIPES ...
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bioRxiv - Biophysics 2023Quote: ... horseradish peroxidase-conjugated purchased from GE Healthcare and used at 1:1000 dilution in 4% milk in PBST ...
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Three-Dimensional Mitochondria Reconstructions of Murine Cardiac Muscle Changes in Size Across AgingbioRxiv - Biophysics 2023Quote: ... A 13-MHz probe (Vivid V echocardiograph; GE Healthcare, Tampa, FL) was used to take standard B-mode M-mode images ...
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bioRxiv - Microbiology 2023Quote: ... followed by phosphor imaging with a Typhoon FLA 700 (GE Healthcare). Kinetic analysis was done as described previously20 ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Molecular Biology 2023Quote: ITC experiments were performed with MicroCal iTC200 (GE Healthcare) at 25°C ...