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Citations for GE Life Sciences :
301 - 350 of 4094 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: SEC and DMC columns were prepared as described previously.4 Sepharose CL-4B (GE Healthcare) and Fractogel EMD SO3- (M ...
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bioRxiv - Molecular Biology 2023Quote: DNA was extracted from leaves of 4 week-old plants by Nucleon Phytopure (GE Healthcare) and total RNA was extracted from 3 week-old plants by RNeasy (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Cell Biology 2024Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Immunology 2024Quote: ... for 30 min at 4°C and with Streptavidin Sepharose High Performance beads (GE Healthcare) for 2h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Supplementary Table 2 for a list of antibodies used in this study ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times in TBST for 5 minutes and visualized using enhanced chemiluminescence (ECL, GE Healthcare). See Extended Data Table 1 for a list of antibodies used in this study ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was carefully layered on top of 4 ml of 90% Percoll (GE Healthcare, Chicago ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were incubated with appropriate antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) or Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Cell Biology 2021Quote: ... Image stacks spanning full ICDs were acquired using a DeltaVision OMX-Blaze (Version 4; Applied Precision) equipped with a 60x PL APO NA = 1.42 objective (Olympus ...
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bioRxiv - Plant Biology 2020Quote: Experiments were conducted at 4°C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in running buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... 0.8% (v/v) immobilized pH gradient (IPG) buffer pH range 4–7 (GE Healthcare, Uppsala, Sweden)] ...
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bioRxiv - Biophysics 2020Quote: ... and subjected to size exclusion chromatography at 4°C using a Superdex-200 column (GE Healthcare) and SEC buffer (50 mM Bis-Tris-Propane pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Equilibrium dissociation constants were obtained at 4°C using a MicroCal VP-ITC system (GE Healthcare). Peptide concentrations between 300-750 μM in the syringe were injected into 25–35 μM pocket domain in the cell ...
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bioRxiv - Biochemistry 2020Quote: ... D1-4 (CNFY1-704) was purified using a HiLoad 16/600 Superdex 200 pg (GE Healthcare) pre-equilibrated in buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... the media was passed through a Sepharose 4 fast flow gel filtration column (GE Healthcare, 170149001), using the AKTA pure chromatography system (GE Health care) ...
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bioRxiv - Immunology 2021Quote: ... which was underlayed with 4 ml 80% percoll solution (GE Healthcare-Life Sciences, Chicago, IL, USA). Tubes were centrifuged (1600 G ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... Cell suspension was then pelleted and then re-suspended in 4 mL 40% Percoll (GE Healthcare), layered on 4 mL 70% Percoll and centrifuged at 800xg (RT ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The in-house resin is prepared by coupling N-hydroxysuccinimide (NHS)-Activated Sepharose 4 (GE Healthcare) with 2-(Pyridyldithio ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... loaded to Sepharose 4 Fast Flow affinity resin column (10 mm x 300 mm; GE Healthcare) mounted to ÄKTA pure system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... or in pGEX-4 T2 plasmid containing an N-terminal GST tag (GE Healthcare/Cytiva; cytivalifesciences.com) have been described previously 71 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification column was prepared using 2-3 mL IMAC Sepharose 6 Fast Flow beads (GE Healthcare) and charged with 0.1 N Nickel sulphate solution ...
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bioRxiv - Cancer Biology 2024Quote: ... the WCLs were incubated with 50 μl 7-methyl-GTP Sepharose 4B beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was loaded on 3 × 5 mL HiTrap™ DEAE Sepharose FF (GE Healthcare, Munich, Germany) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...