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Citations for GE Life Sciences :
151 - 200 of 4094 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000-20000 rpm at 4 °C and supernatant was allowed to bind to Glutathione resin (GS resin) (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat no. 17-5132-02) in a batch binding mode for overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... to 2 ml and subsequently loaded on a Sepharose 4 Fast Flow resin column (10 mm x 300 mm; GE Healthcare, Little Chalfont, UK), connected to the ÄKTA pure system (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Biochemistry 2020Quote: ... SDS-PAGE gels (15-well NuPAGE 4–12% BisTris, GE Healthcare) revealed a single band for the purified enzymes ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose 4 Fast flow beads (GE Healthcare Life Sciences) washed twice with wash buffer were added to each IP and incubation was continued for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and Protein G Sepharose 4 Fast Flow Media (#11524935, GE Healthcare) to pull-down phosphorylated IRF5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NHS-activated Sepharose 4 Fast Flow was obtained from GE Healthcare and protein ladder from Thermo Fisher Scientific.
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bioRxiv - Genetics 2021Quote: ... Glutathione Fast Flow Sepharose 4 beads (GE Healthcare, 17-5132-01) that had been pre-washed in binding buffer were then added to the binding reaction and it was incubated in a rotor for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose 4 Fast Flow (17-0618-01, GE Healthcare), Dynal magnetic beads (110.31 ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C using an ÄKTA Pure 25 M (GE Healthcare). The column was washed with 75 mM imidazole [in 20 mM NaH2PO4 and 0.5 M NaCl (pH 7.4)] ...
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bioRxiv - Biophysics 2023Quote: ... and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare), and Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: 30 µl Protein G Sepharose 4 Fast Flow beads (GE Healthcare) were washed with 1 ml of PBS and twice with 1 ml of PBS/1% BSA ...
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bioRxiv - Cell Biology 2024Quote: NHS-Activated Sepharose 4 Fast Flow (GE Healthcare; Catalog number: 17090601)
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bioRxiv - Cell Biology 2024Quote: NHS-Activated Sepharose 4 Fast Flow (GE Healthcare; Catalog number: 17090601)
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in 4 ml of 40% Percoll (GE Healthcare) and layered onto 2.5 ml of 80% Percoll in a 15 ml tube ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Biochemistry 2021Quote: ... the purified OxlT was mixed with purified D5901-A08-Fab at a 1:1.3 molar ratio at 4 °C overnight and applied to a HiLoad 16/60 Superdex200 pg column (GE healthcare) using buffer D (20 mM MES-KOH ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Swarmer cells were isolated from the culture by centrifugation (20 mins at 11,000 rpm, 4°C) after mixture with 1 volume of Percoll (GE Healthcare). The synchronized swarmer cells were pipetted onto an agarose (2% ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded at low speed (0.2 ml/min) at 4°C onto a HisTrap-HP 1 mL affinity column (GE Healthcare) equilibrated with buffer A (20 mM NaPi pH 7.7 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation (15’000 xg, 4 °C, 30 minutes) and supernatants were incubated with 1 ml Glutathione Sepharose (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3-5 minutes and visualized using Amersham Imager 600 (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein purification was performed with Glutathione Sepharose 4 Fast Flow (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and NHS-activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences, IL), was added to the sample ...
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bioRxiv - Cell Biology 2021Quote: ... precleared with protein G-Sepharose 4 beads (GE Healthcare #17-0618-0), were incubated with an anti-Flag monoclonal antibody or anti-GFP polyclonal for 1 hr ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μL of protein-G sepharose-4 fast flow (GE Life Sciences) (pre-washed and suspended in ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 4-mL GST column (GE Healthcare) equilibrated in PBS containing 1 mM EDTA ...
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bioRxiv - Immunology 2022Quote: ... or Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Sepharose 4 Fast flow beads (GE Healthcare, GE17-0618-01) were incubated with α-DAF (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... 4-20% (p/v) Amersham ECL Gel (GE Healthcare, Chicago, Illinois, EEUU), using the ECL Gel Box system (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... were covalently immobilized on NHS-activated Sepharose 4 Fast Flow (GE Healthcare) and packed into tip columns for the RCC instrument ...
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was purified over Glutathione Sepharose 4 Fast Flow (GE Healthcare) and eluted from the beads using 50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2023Quote: ... and subsequently purified using Protein G Sepharose 4 Fast Flow (GE Healthcare), eluted using IgG elution buffer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... or with protein G Sepharose 4 fast flow slurry (GE Healthcare #17061801) for each immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was incubated with 4 ml of IgG Sepharose (GE Healthcare) for 6 hours ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and chromatin was released from the resin with 20 µl of ChIP buffer 1 supplemented with 100 µg/ml BSA and 4 µg HRV3C incubated (GE Healthcare) on ice for 60 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...