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Citations for GE Life Sciences :
201 - 250 of 4094 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in HRP conjugated secondary antibodies detailed in Table 4 for 1 h at room temperature and developed using Pierce SuperSignal Pico Plus (Pierce) or ECL (GE Healthcare) reagent and imaged on ImageQuant.
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to the ATCC guidelines in 4:1 mixture of Dulbecco’s medium and Medium 1999 supplemented with 10% Hyclone™ FBS (GE Healthcare) and 50μg/ml Gentimicin (Life Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM NaN3) on a Superdex 200 Increase 5/150 column (GE Healthcare) equilibrated by the same buffer and run at a 0.45 ml/min flow rate ...
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bioRxiv - Biophysics 2022Quote: ... The TOP3A-RMI1/2 complex was assembled by incubating purified TOP3A and RMI1/2 (1:3 ratio) followed by purification using a Superdex 200 Increase (GE Healthcare) in buffer I.
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Microbiology 2022Quote: ... rolling at 4°C with 100 μl of Ni-NTA Agarose (GE Healthcare) pre-equilibrated with the SEC buffer ...
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bioRxiv - Biochemistry 2021Quote: Purification was performed at 4°C using an ÄKTA™ pure (GE Healthcare) to control flow and column pressure ...
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bioRxiv - Microbiology 2020Quote: ... 100 µl of Glutathione Sepharose 4 Fast Flow (GE Healthcare, #GE17-5132-01) were packed onto Poly-Prep chromatography columns (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... it was applied to a Con A-Sepharose 4 B column (GE Healthcare), and glycoproteins were eluted with 0.5 M α-D-methylglucoside ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysates were then incubated with Glutathione Sepharose 4 Fast Flow (GE Healthcare) to immobilize GST-fusion proteins.
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bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled overnight at 4°C to protein A Sepharose beads (GE Healthcare). Coupled beads were washed three times in EB buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2021Quote: ... The chromatography was undertaken at 4°C using an ÄKTA FPLC (GE Healthcare). The clarified supernatant was loaded onto a 5 mL prepacked Ni-nitrilotriacetic acid (NTA ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C in the presence of Protein A beads (GE Healthcare). ChIP-Seq libraries were prepared using the Kapa LTP Library Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied along with Protein G Sepharose 4 Fast Flow beads (GE Healthcare). All ChIP extracts were pre-cleaned for 1 hr with 30 μl beads on a rotor at 4°C in absence of the antibody ...
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bioRxiv - Biochemistry 2023Quote: ... and Benzamidine Sepharose 4 Fast Flow were obtained from GE Healthcare (Uppsala, Sweden). HPE-IAM was obtained from Molecular Biosciences (Boulder ...
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bioRxiv - Physiology 2023Quote: ... The transfection mix consisted of 4 μL of DharmaFECT1™ (GE Healthcare, UK), 144 μL of 1 μM siRNA (35 nM final concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 mL of Amersham™ ECL Prime Western Blotting Detection Reagent (GE HealthCare) was added to each membrane in the dark for 1 min ...
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bioRxiv - Physiology 2023Quote: ... using a multifrequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare) was used to capture right and left leg images to determine VL muscle cross-sectional area (mCSA) ...
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bioRxiv - Biophysics 2022Quote: ... samples were loaded at 4 °C onto a Resource S column (GE Healthcare) equilibrated in 20 mM MES-NaOH pH 6.0 (for reactions containing FimCHisI or FimCHisIA) ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was resuspended in 4 ml of 80% Percoll (GE Healthcare), added to a 15 ml falcon tube ...
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bioRxiv - Biophysics 2023Quote: ... Runs were performed at 4°C through an ÄKTAmicro (GE Healthcare Life Sciences) system.
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bioRxiv - Cancer Biology 2024Quote: Immunoprecipitations were performed using activated CNBr beads (Sepharose 4 Fast Flow, GE Healthcare). 0.5 g beads were solved in 5 ml 1mM HCl and incubated at RT for 20 min ...
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bioRxiv - Plant Biology 2024Quote: ... After the proteins were purified by Glutathione Sepharose 4 Fast Flow (GE Healthcare) and Amylose Resin (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Molecular Biology 2024Quote: ... and applied to 2 × 5-mL StrepTrap HP columns (GE Healthcare), connected in series ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...