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Citations for GE Life Sciences :
301 - 350 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... 24 cm (GE Healthcare Bio-Sciences, Piscataway, NJ) was used ...
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bioRxiv - Cancer Biology 2024Quote: Surface plasmon analysis was performed using the Biacore-T-200 system (GE Healthcare). Both recombinant PRMT5-MEP50 complex and EZH2 (50µg ...
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bioRxiv - Cell Biology 2024Quote: ... Raw images were acquired using a 0.2 μm z-step size and reconstructed in three dimensions with the softWoRx 5.0.0 (Applied Precision) software ...
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bioRxiv - Cell Biology 2024Quote: Fluorescence images were obtained using a DeltaVision Elite system (GE Healthcare Inc.) equipped with a pco.edge 4.2 sCMOS camera (PCO ...
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bioRxiv - Cell Biology 2024Quote: ... peptides were loaded to an Immobiline™ DryStrip Gel (GE Healthcare, #11534985) isoelectric focusing (IEF ...
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bioRxiv - Biophysics 2024Quote: ... Pooled fractions were loaded onto a 5 ml Q-HP column (GE Life Sciences) equilibrated in buffer A and washed with 5% buffer B ...
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bioRxiv - Biophysics 2024Quote: ... and impurities were removed using a Superdex 75 column (GE Life Sciences). Protein pellets were resuspended in 4 ml of TBS buffer with protease inhibitor and further purified by size-exclusion chromatography on a Sephacryl 26/60 column pre-equilibrated with buffer containing10mM potassium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified and buffer exchanged by size exclusion chromatography using HiLoad 16/600 Superdex-G-200 (GE Healthcare) gel filtration column to storage buffer (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Biophysics 2024Quote: ... The soluble fraction of gp24 was incubated with glutathione sorbent slurry (Glutathione Sepharose 4B; GE Healthcare Life Sciences), washed with phosphate buffer ...
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bioRxiv - Biophysics 2024Quote: ... filtered (0.42 pore size) and subsequently loaded onto a HiTrapQHP anion exchange chromatography column (GE Healthcare). The protein was eluted using a gradient with 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... The secondary antibodies used were a sheep α-mouse antibody conjugated to horseradish peroxidase (HRP) (GE Life sciences, Marlborough MA) at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences ...
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bioRxiv - Biophysics 2024Quote: ... the complex was purified by a Superdex 200 10/300 column (GE Healthcare) equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were loaded onto a Superdex 200 Increase 100/300 GL column (GE Healthcare) and the correct fractions were pooled ...
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bioRxiv - Biophysics 2024Quote: ... The filtrate was passed through StrepTrap HP 5 mL column (GE Healthcare) using 100 mM Tris-HCl pH 8.0 and 150 mM NaCl (Buffer A) ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the protein were collected and concentrated using 100 kDa cutoff concentrator and loaded onto Superdex 200 10/300 GL column (GE Healthcare) using 20 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... followed by a Superdex 200 column (GE healthcare) in the same buffer.
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bioRxiv - Biophysics 2024Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution step ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified by size-exclusion chromatography using a Superdex 200 column (GE healthcare) in SEC buffer (25 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... The different phosphorylation states of PKA-CF100A were separated using a cation exchange column (HiTrap Q-SP, GE Healthcare Life Sciences) using a linear gradient of KCl in 20 mM KH2PO4 at pH6.5.83 The purified protein isoforms were then stored in phosphate buffer containing 10 mM dithiothreitol (DTT) ...
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bioRxiv - Biophysics 2024Quote: All SPR binding experiments were performed on a CM5 chip (Cytiva) at 25 °C using a Biacore T200 Instrument (GE Healthcare). The purified CNBH domains were immobilized on the CM5 chip using a standard amine coupling chemistry in the presence of 10 mM sodium acetate buffer at pH 5.5 as the immobilization buffer (buffer used to directly dissolve ligands) ...
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bioRxiv - Biophysics 2024Quote: ... Cleaved ProTα was purified further using Ni Sepharose Excel resin (Cytiva, formerly GE Healthcare; Søborg, Denmark) and a HiPrep Q FF column (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... Quantification of the signals was performed using ImageQuant TL (GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... Abl83-534 was then purified using an ion exchange Q-sepharose HP (GE Healthcare) column equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... The eluate was concentrated and applied onto a Superose 6 Increase 10/300 column (GE Healthcare) for size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2024Quote: ... A total of 250 µL of each protein was injected and run on a Superdex 200 Increase (S200) 10/300 column (GE Healthcare) at a flow rate of 0.40 mL/min in SEC-MALS buffer (10 mM Tris pH 8.3 ...
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bioRxiv - Biophysics 2024Quote: ... The truncated Abl mutants did not bind to either ion exchange Q- or S-sepharose HP (GE Healthcare) columns in ion exchange buffer ...
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bioRxiv - Biophysics 2024Quote: ... was fed into the X-ray beam after passing through a Superdex 200 (S200) Increase 10/300 GL column (GE Healthcare) in SAXS buffer (50mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The sample was again filtered to 0.2 µm and loaded onto a HiLoad 16/600 Superdex 75 column (GE Healthcare) and eluted as a monomer ...
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bioRxiv - Biophysics 2024Quote: ... connected to an AKTA Pure FPLC (GE Healthcare). Under a flow rate of 0.5 mL/min ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Biophysics 2024Quote: ... the protein was concentrated to approximately 500 µL using centrifugal spin filters (Milipore) and injected over a Superdex 200 (S200) Increase 10/300 column (GE Healthcare) connected to an AKTA Pure FPLC (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by ECL (GE Healthcare). DNA loading was assessed by re-probing the membrane with radiolabeled mouse genomic DNA with ethidium bromide (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were obtained from GE Healthcare (Chicago, IL, USA). Bound antibodies were detected by ECL (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... Amersham ECL Prime Western blotting detection reagent (GE Healthcare) was used to detect immunoreactive bands ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected on a LAS-4000 (GE Healthcare) in SYBR-green fluorescence detection mode and Image Quant TL software.
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bioRxiv - Cell Biology 2024Quote: ... 300 µg of total protein extracts were purified using Streptavidin Sepharose High-Performance beads (GE Healthcare Bio-Sciences AB). For western blot analysis ...
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bioRxiv - Cell Biology 2024Quote: ... and subjected to SDS-PAGE before transfer to nitrocellulose (GE Healthcare). Membranes were blocked in PBS containing 5% milk (PBS-M ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... and goat-anti-mouse (GE Healthcare) secondary antibodies were used at concentrations of 1:10,000.
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bioRxiv - Cancer Biology 2024Quote: ... 15 mL of Ficoll-Paque™ Plus (#17-1440- 03, GE Healthcare,) was dispensed to a 50 mL SepMate™ (#85450 ...
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bioRxiv - Cancer Biology 2024Quote: ... DRG neurons were further purified using a Percoll (GE Healthcare, 17-0891-01) gradient (12.5% and 28%) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3-5 minutes and visualized using Amersham Imager 600 (GE Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... CM5 Sensor chip for SPR (cat no: 50-105-5511) was purchased from GE Healthcare Bio-Sciences (Cytiva life sciences-USA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Band intensities were quantified using an ImageQuant LAS 500 system (GE Healthcare, IL, USA). The same experiments were repeated for spheroids and organoids treated with Bevacizumab or transfected with miR200c ...
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bioRxiv - Cancer Biology 2024Quote: ... the corrected sensograms were analysed by employing BIA-T200 evaluation software version 2.1 (GE Healthcare). The relation between protein concentration and the Response unit (RU ...
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bioRxiv - Cell Biology 2024Quote: Live imaging experiments were carried out on the DeltaVision Elite microscope (GE healthcare) or the CSU-W1 SpinningDisk (Nikon ...
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bioRxiv - Cell Biology 2024Quote: ... Images were collected as a Z-stack and deconvolved using SoftWorx (GE HealthCare), then displayed as a maximum intensity projection ...