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Citations for GE Life Sciences :
101 - 150 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was fitered and protein purification was conducted using a Resource Q column (GE Healthcare) equilibrated in Buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... calibrated with High Molecular Weight calibration kit (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... Protein purification was conducted using a Resource Q column (GE Healthcare), eluting TET2 using a linear gradient of Elution buffer over 10 column volumes ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Buffer exchange was performed via PD-10 desalting column (GE Healthcare) to 50mM MES pH 5.0 for experiments and storage ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... loaded onto a 5 mL Heparin column (HiTrap Heparin HP, GE Healthcare Life Sciences), and washed with a heparin dilution buffer supplemented with 10 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Gel images were scanned using a Typhoon phosphorimager (GE Healthcare) and were quantified using ImageJ.
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bioRxiv - Biochemistry 2024Quote: ... The Spt4/5 heterodimer was further purified by size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare) through isocratic elution in low salt buffer ...
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bioRxiv - Biochemistry 2024Quote: ... we used a NICK column (GE Healthcare), and fractions were collected manually ...
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bioRxiv - Biochemistry 2024Quote: ... the filtrate was applied to a HisTrap™ 5 ml Fast Flow (Cytiva) equilibrated in lysis buffer using an Äkta Purifier system (GE Healthcare). The column was washed with lysis buffer (100 mM Tris/HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was loaded on a Superose6 increase 10/300 (GE Healthcare) gel filtration column pre-equilibrated with Buffer A containing 0.5 mM DDM and 0.5 ml fractions were collected ...
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bioRxiv - Biochemistry 2024Quote: ... The elution containing NRG1ý was concentrated with a 3K cutoff and applied on an S200 10/300 increase column (GE Healthcare). Protein content of the major peak was stored in aliquots at -80 °C for subsequent receptor purifications.
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bioRxiv - Biochemistry 2024Quote: ... The molecular weight of protein complexes was determined after size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The concentrated solution was loaded onto a HiLoad 16/60 Superdex 200 column (GE Healthcare) equilibrated with Dialysis buffer ...
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Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: ... and pure HDAC8 enzyme was obtained using the HisTrap column on AKTA purifier UPC 10 (GE Healthcare Life Sciences). SDS-PAGE agarose gel and catalytic activity analysis confirmed the presence of the HDAC8 enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... were covalently immobilized on NHS-activated Sepharose 4 Fast Flow (GE Healthcare) and packed into tip columns for the RCC instrument ...
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bioRxiv - Biochemistry 2024Quote: ... then loaded onto a 1 ml HiTrap heparin HP column (GE Healthcare) pre-equilibrated in low salt buffer and eluted with a linear gradient of 100 mM to 1 M KCl over 25 CVs.
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bioRxiv - Biochemistry 2024Quote: The quaternary structures were determined by size exclusion chromatography using a 25 mL Superdex 200 10/300 GL Increase column (GE Healthcare) for FDP_E ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was then purified by gel filtration chromatography (Superose 6 10/300; GE Healthcare) in dialysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEx-6p-1 (GE Healthcare) digested with the same restriction enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... loaded onto an anion-exchange column (HiTrap Q HP, GE Healthcare Life Sciences), washed with an anion-exchange dilution buffer supplemented with 30 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was further purified by Q Sepharose column chromatography (GE Healthcare) and gel-filtration column chromatography (HiLoad Superdex75 16/60 ...
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bioRxiv - Biochemistry 2024Quote: ... and gel-filtration column chromatography (HiLoad Superdex75 16/60, GE Healthcare). The purified protein was concentrated to 15 mg/ml and exchanged into buffer containing 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2024Quote: ... Origin substrates were amplified using standard PCR protocols and were purified by anion exchange chromatography using a 1 ml RESOURCE Q column (GE healthcare). Peak fractions were ethanol precipitated and resuspended in TE buffer.
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Signals were detected using the AI600 Imager (Amersham) and quantified using ImageQuant™ TL software (GE Healthcare).
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... The signal was acquired using Typhoon Trio™ Variable Mode Imager (GE Healthcare) and analyzed in ImageQuant™ TL software.
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bioRxiv - Biochemistry 2024Quote: ... and the labeled enzymes were detected using Cy3 /TAMRA settings (excitation wavelength 532 nm) in a Typhoon FLA9500 phosphor imager (GE Healthcare Life Sciences). Further ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: Non-specifically binding proteins were cleared from each sample by incubation with 60 µL of 20% Protein A-Sepharose™ CL-4B (GE Healthcare) in IP Buffer (150 mM NaCl / 1% Triton X-100 / 15 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: Speedbead magnetic carboxylate-modified particles (GE45152105050250 and GE65152105050250, GE Healthcare, Chicago, IL) were combined in a 1:1 (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... further size exclusion chromatography was performed in a Superose 6 10/300 column (GE Healthcare) for the removal of dephosphorylation reagents.
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bioRxiv - Biochemistry 2024Quote: ... A total reaction mixture of 500 µL was used for downstream size exclusion purification utilizing a Superose 6 10/300 column (GE Healthcare) to remove excess PKA and phosphorylation reagents ...
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bioRxiv - Biochemistry 2024Quote: ... Further purification occurred by subjecting the samples to an anion exchange column (HiTrap SP, Cytiva, 17115201) followed by size-exclusion chromatography (HiLoad 16/60 Superdex S75; GE Healthcare, 17106801) as previously described (12) ...
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bioRxiv - Biochemistry 2024Quote: ... Size exclusion chromatography (SEC) using a HiLoad Superdex 75 pg 16/60 column (GE Healthcare) was used to separate the cleaved protein from any contaminants ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved hP0 was injected into an S200 Increase 10/300 GL column (GE Healthcare), and the peak corresponding to pure monomeric hP0 was collected and concentrated using Vivaspin centrifugal concentrators (Sartorius).
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bioRxiv - Biochemistry 2024Quote: ... and the resulting Fluo-Ub was purified using a Superdex 75 size-exclusion column 10/300 GL (GE Healthcare) in buffer (20 mM HEPES ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... ion exchange (HiTrap Heparin column, GE Healthcare), and size-exclusion chromatography (Superdex 200 10/300 GL column ...
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bioRxiv - Biochemistry 2024Quote: ... and size-exclusion chromatography (Superdex 75 10/300 GL column, GE Healthcare). UbcH5c was purified by Ni affinity chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... After overnight exposure to a storage phosphor screen (BAS-IP MS 2025 E GE Healthcare) the signal was detected using a Typhoon FLA7000 phosphoimager.
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bioRxiv - Biochemistry 2024Quote: ... and further purified via a gel-filtration column (Superdex 200 10/300 GL, GE Healthcare), which was equilibrated with an RNase-free gel-filtration buffer (10 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were exposed to a phosphor screen and then scanned by a Typhoon Phosphor Imager (GE Healthcare). The gel image was quantified by ImageJ (33) ...
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bioRxiv - Biochemistry 2024Quote: ... which was scanned by a Typhoon Phosphor Imager (GE Healthcare). The gel image was quantified by ImageJ (33) ...
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bioRxiv - Biochemistry 2024Quote: ... and detected using a high-sensitivity imaging system (LAS-4000, GE healthcare, Chicago, IL, USA).
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bioRxiv - Biochemistry 2024Quote: ... consisting of a Protran nitrocellulose membrane (Whatman) followed by a Hybond N+ nylon membrane (GE Healthcare Bioscience). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...