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Citations for GE Life Sciences :
251 - 300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The protein eluent was concentrated to 500 µL and subjected to size-exclusion chromatography on a Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated with Buffer D (25 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) preequilibrated with size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: All live cell microscopy was performed on a DeltaVision Elite Deconvolution microscope (Applied Precision, Issaquah, WA, USA). Imaging pads were stained with 8 µL of dye mix (25 µg/mL DAPI ...
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bioRxiv - Biochemistry 2024Quote: ... membranes were washed and incubated with secondary anti-mouse peroxidase-conjugated antibodies (GE Healthcare NA931V) for 1h ...
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bioRxiv - Biochemistry 2024Quote: ... supernatant was loaded onto a pre-packed 5 ml HisTrap column (GE Healthcare), equilibrated according to the supplier’s protocols with wash buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Biochemistry 2024Quote: ... TBC-DEG-αβ-tubulin assemblies were purified by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE healthcare) in binding buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... and images were captured on ImageQuant LAS 4000 (GE Healthcare) device ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were revealed using the ECL kit (GE Healthcare RPN2232), and images were captured on ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... gels were imaged using Typhoon-9400 scanner (GE Healthcare) with a resolution of 100 μm using appropriate emission and excitation wavelengths ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was quenched with 1 μl 14.3 M βME followed by final buffer exchange using size exclusion chromatography (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The second dimension run was performed in an EttanDalt Six Electrophoresis Unit (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: Relative protein spot quantification across experimental conditions was performed using DeCyder v7.0 software and multivariate statistical module EDA v7.0 (Extended Data Analysis, GE Healthcare), as follows ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM βME was added to reduce cysteine residues followed by buffer exchange using a HiTrap 26/10 Desalting column (GE Healthcare) in 25 mM HEPES (pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Glutathione Sepharose 4B (GE Healthcare). GST was cleaved from protein by TEV protease treatment for 16 hours at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The purified receptor was subsequently concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and run on size-exclusion chromatography on a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: A second purification step on Ni-NTA beads was performed to remove any uncleaved protein before to achieve a SEC purification onto a Superdex 75 16/600 GE column (GE Healthcare) in a final buffer containing 10 mM Tris pH = 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... and loaded onto a HisTrap chelating column (GE Healthcare) running on an AKTA™ FPLC workstation ...
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bioRxiv - Biophysics 2024Quote: To analyze the VWFA1-GPIbα interaction by surface plasmon resonance (SPR; Biacore 3000; GE Healthcare), human recombinant GPIbα headpiece followed by 133 residues of the SV40 large T antigen with Cys residues mediating dimerization was generated ...
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bioRxiv - Biophysics 2024Quote: ... we loaded the protein solution onto a HiTrap Q FF column (GE Healthcare) and eluted it with a linear gradient of 0–1 M NaCl in 20 mM sodium phosphate at pH 6.0 ...
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bioRxiv - Biochemistry 2024Quote: ... the soluble fraction was incubated in batch for 2 h with glutathione Sepharose 4B (GE Healthcare), previously equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
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bioRxiv - Biochemistry 2024Quote: ... ImageQuant TL (GE Healthcare) was used for image quantification ...
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bioRxiv - Bioengineering 2024Quote: ... which was transferred to the Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Bioengineering 2024Quote: ... Radioactive probes were prepared by random-primed 32P labeling (GE Healthcare, Chicago, IL) of gel-purified DNA fragments of the following genes ...
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bioRxiv - Bioengineering 2024Quote: ... Hybridazation results were imaged with a Typhoon RGB scanner (GE Life Sciences, Chicago, IL) and quantified with ImageJ software (version 2.0.0-rc-38/1.50b).
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bioRxiv - Biochemistry 2024Quote: ... eluted protein was loaded onto a HiPrep 26/10 desalting column (GE Healthcare) containing 25 mM Tris pH 7.5 at 20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The density of the bands corresponding to concerted integration on fluorescence-scanned gels was quantified by ImageQuant (GE Healthcare). The integration activity of WT IN was set to 100% ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was visualized by fluorescence using a Typhoon 8600 fluorescence scanner (GE Healthcare). Densitometry was used to quantify IN activity ...
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bioRxiv - Biochemistry 2024Quote: ... Multimeric state of WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) equilibrated with running buffer at a flow rate of 0.2 ml/min.
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bioRxiv - Biochemistry 2024Quote: ... The reaction mix was loaded onto a HisTrap column (GE Healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: The gels were scanned with an Amersham Typhoon RGB Biomolecular Imager (GE Healthcare Life Sciences) to visualize the fluorophore-labeled primer and extended primer bands ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Biochemistry 2024Quote: ... Autoradiography was performed with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences), and images were quantified using ImageQuant TL software (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... The middle two layers were removed for subsequent desalting (using five connected Hi-Trap desalting columns (GE Healthcare, Chicago, IL, USA) on an AKTA Pure FPLC system) ...
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bioRxiv - Bioengineering 2024Quote: ... and transferred onto a nitrocellulose membrane (Amersham Protan, GE Healthcare, #10600012). Proteins were visualized by ECL western blotting detection reagents (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... Purification and fractionation steps were performed with an “ÄKTApurifier” FPLC system (GE Healthcare). The filtrate was loaded onto a HiTrap DEAE FF column (5 mL ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM 2-mercaptoethanol and desalted into the same buffer using a 5 mL HiTrap desalting column (GE Healthcare) using an AKTA FPLC system (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... HiLoad16/600 column (GE Healthcare). Elution fractions containing pure protein were determined via SDS-PAGE analysis on a 4-20% gradient gel (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was concentrated to a total volume of 2.5 mL and desalted using PD-10 desalting columns (GE Healthcare) and desalting buffer (25 mM Tricine-NaOH ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... Proteins were further purified by concentrating to ∼3mg/ml and applying to a size exclusion column (Superdex-200 increase, 10/30 GE Healthcare) equilibrated in buffer A containing 5 mM ATP ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... protein was loaded onto a pre-equilibrated (50 mM Tris-HCl 7.5 at 4 °C and 100 mM sodium chloride) Superdex 16/600 size exclusion column (GE Life Sciences, Chicago, IL) following Ni-NTA affinity chromatography to remove any protein aggregate ...