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Citations for GE Life Sciences :
501 - 550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and imaged using an Amersham imager 600 (GE Healthcare, Chicago, IL). Membranes were stripped using Restore™ PLUS Western Blot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... electrotransferred onto nitrocellulose membranes (GE Healthcare, LC, UK), and probed with rabbit PYK2 antibodies (diluted 1/1 000 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Plant Biology 2024Quote: ... Protein content were estimate using 2D QuantKit (GE Healthcare) and adjusted to 2 µg/µl ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were incubated in ECL Western Blotting Substrate (GE Healthcare; RPN2106) before imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Imaging was performed on a widefield microscope (Deltavision, Applied Precision) with a 60X objective (NA 1.42 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Both chemiluminescence and pre-stained size marker images were captured in the same field of view using ImageQuant 800 (AMERSHAM/GE Healthcare Japan, Tokyo, Japan). For quantification ...
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bioRxiv - Neuroscience 2024Quote: ... immunoreactivity was detected using the Enhanced chemiluminescence (ECL) system (GE Healthcare, Tokyo, Japan). Both chemiluminescence and pre-stained size marker images were captured in the same field of view using ImageQuant 800 (AMERSHAM/GE Healthcare Japan ...
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bioRxiv - Neuroscience 2024Quote: Whole-brain imaging was performed using a 3T GE scanner (GE HealthCare, United States) with a 16-channel head coil ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... Phase contrast and epifluorescence images to detect mTurquoise2 fluorescence were acquired on a DeltaVision Elite V3 inverted fluorescence microscope (GE Healthcare) with a CFP filter set (excitation = 400-453 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... Three-dimensional structured-illumination microscopy (3D-SIM) was done using a DeltaVision OMX SR (GE Healthcare) microscope with a 60X plan Plan 1.42 NA lens ...
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bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... The active fraction (AGH1) was further purified to homogeneity by size exclusion chromatography using a Superdex Peptide HR 10/30 (Amersham Biosciences, GE Healthcare). Elution was performed under isocratic conditio ns with 30% ACN in acidified water at a flow rate of 0.5 mL/min ...
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bioRxiv - Microbiology 2024Quote: ... The mutated proteins were purified from the media using a Protein-A affinity column (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the cleaved protein was removed and applied to a size exclusion (SEC) column (HiLoad_16/60_Superdex75 prep- grade, GE Healthcare) equilibrated with PBS ...
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bioRxiv - Microbiology 2024Quote: ... 500 µl elution were collected and PD MiniTrap G-25 columns (GE Healthcare) were used to exchange buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... and exposed using Amersham imager 680 (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... protein was further purified by size exclusion chromatography on a HiPrepTM 26/60 SephacrylTM S200 HR gel filtration column (GE Healthcare). Protein was eluted isocratically at a flow of 1 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... exposed to a phosphorimager screen and revealed by autoradiography using a Typhoon phosphorimager (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... the RNA was transferred onto nylon membranes (Amersham Hybond N+, GE Healthcare) and subjected to hybridization as previously described(Fricker et al ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered (0.45 polyvinylidene fluoride membrane, Carl Roth, Germany) and applied onto a 1 ml Nickel-IDA column (GE Healthcare) equilibrated in 50 mM TRIS-HCl pH 7.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary mouse (NA931V) and rabbit (NA934V) antibodies from GE healthcare. Data were analyzed with Fusion Solo software (Vilberg ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were treated with enhanced chemiluminescence detection reagents (Chemi-Lumi One Super; Nacalai Tesque) and exposed to ECL Select LAS500 (GE Healthcare, Buckinghamshire, UK). All images were scanned with Image J software.
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bioRxiv - Cancer Biology 2024Quote: ... Separated proteins were transferred to Hybond™ nitrocellulose membranes (GE Healthcare) using a semi-dry method (Bio-Rad Trans-Blot® Turbo™ Transfer System) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (HyClone, GE Healthcare Life Sciences, Logan, UT) and 0.5% penicillin-streptomycin (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and PC3M cells were cultured in Dulbecco’s modified Eagle’s medium (HyClone, GE Healthcare Life Sciences, Logan, UT) supplemented with 10% fetal bovine serum (HyClone ...
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bioRxiv - Cancer Biology 2024Quote: ... PBMCs were separated from the blood using a Ficoll density gradient (GE Healthcare #17144002). CD4 or CD8 cells were isolated using negative selection Miltenyi Biotec kits ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to a nitrocellulose membrane (cat. no. 10600009, GE Healthcare). Blotting efficiency was assessed by Ponceau membrane staining (P7170 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal was developed using Amersham ECL Prime Western blotting Detection reagent (GE Healthcare, Chicago, IL, USA), and images taken using a G:BOX gel imaging system (Syngene ...
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bioRxiv - Cancer Biology 2024Quote: ... Imaging was performed using the IN Cell Analyzer 6000 (GE Healthcare). Five to six fields of view ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
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bioRxiv - Cancer Biology 2024Quote: ... Developer Toolbox (GE Healthcare) were used for image analysis.
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bioRxiv - Cancer Biology 2024Quote: ... ICY and Developer Toolbox (GE Healthcare) were used for image analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Immunofluorescence cell images were acquired on a DeltaVision Core deconvolution microscope (Applied Precision) equipped with a CoolSnap HQ2 CCD camera ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were visualized using the Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... 15-25μg of proteins were run on NuPage 4-12% gradient Bis-Tris gels in NuPAGE™ MES SDS Running Buffer (20X) and wet-transferred to 0.2μM nitrocellulose membranes (GE Healthcare, #10600001). Blots were blocked in Intercept (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... DAPI-stained cells were observed using Delta Vision system (Applied Precision) equipped with a 100x oil-immersion lens (Plan Apo ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were imaged using the IN Cell 6500HS imaging system (GE Healthcare), the images were analysed using Cell Profiler version 4.2.1 (Broad institute of MIT and Harvard ...
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bioRxiv - Microbiology 2024Quote: The same mixture of phage DNA as mentioned above was amplified by Multiple Displacement Amplification (MDA) using the Genomephi V3 kit (GE Healthcare Life Science, Marlborough, MA, USA) with 10 ng input and incubated at 30°C for 0.5 h (MDA_0.5h ...
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bioRxiv - Microbiology 2024Quote: ... The resulting lysate underwent clarification through centrifugation (125,000g for 30 minutes at 277 K) using a Ti-45 rotor (Beckmann) and was subsequently applied to a 1 ml HisTrap HP column (GE Healthcare). The column underwent an initial wash with five column volumes of lysis buffer containing 40 mM imidazole at pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... and then subjected to size-exclusion chromatography using either an S75/26–60 column or an S200/26–60 (GE Healthcare) in the same buffer as before but without imidazole ...
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bioRxiv - Microbiology 2024Quote: ... affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare) and then concentrated to an optical density at 280 nm (OD280 ...
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bioRxiv - Microbiology 2024Quote: Analytical size-exclusion chromatography was performed using an AKTA fast protein liquid chromatography (FPLC) system (Amersham GE Healthcare). The concentrated samples were loaded into a Superose12 HR 10/30 FPLC column (GE ...
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bioRxiv - Microbiology 2024Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare). Quantification of the EtBr and radioactive signals was performed with Fiji software (80) ...