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Citations for GE Life Sciences :
3101 - 3150 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... Cells were recorded by acquiring one field of view per well in a 96-well black plate (GE healthcare). We used our previously developed semi-automated pipeline for segmentation and tracking of individual cells5.
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bioRxiv - Genomics 2019Quote: ... for 10 minutes at room temperature and subsequently washed and imaged on an IN Cell Analyzer 2200 (GE Healthcare). Images were background-subtracted using FiJi (Schindelin et al. ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Clarified cell supernatants were concentrated 10-fold using Kicklab tangential flow filtration cassettes (GE Healthcare, cut-off 10 kDa) and adjusted to pH 8.0 in 10 mM Tris before purification in an AKTA Avant system by Steptrap-HP chromatography (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... All primary antibody incubations were followed by incubation with appropriated secondary HRP-conjugated antibodies (GE Healthcare or Cell Signaling) in 5% milk plus 0.1% Tween20 (Sigma P2287) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (GE Healthcare, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (PS ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Time-lapse live cell imaging was carried out with the GE DV Elite Applied Precision DeltaVision system (GE Healthcare) equipped with Olympus oil objectives of 60X (NA 1.42 ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides from whole cell samples were focused on immobilized pH gradient (IPG) gel strips (GE Healthcare, Waukesha, WI, USA) on a linear pH range of 3-10 ...
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bioRxiv - Biochemistry 2020Quote: ... Immunofluorescent intensities of H3K9Ac mark in the nuclei (total ~ 2500 – 4500 nuclei/treatment) were quantified by high-content image analysis (IN Cell Analyzer Workstation 3.7.2, GE Healthcare). H3K9 acetylation levels were reported after the intensities of the acetyl mark were normalized to DMSO-treated samples ...
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bioRxiv - Neuroscience 2020Quote: HEK293T and Neuro-2a cells were grown in DMEM (Biowest, L0104) supplemented with 10% FBS (GE Healthcare Hyclone, 12350273).
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bioRxiv - Microbiology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were purified from buffy coats by density gradient centrifugation (Ficoll-Paque Plus; GE Healthcare) and then cryopreserved in FBS with 10% DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were enriched by density gradient separation using Ficoll (Ficoll-Paque™ plus, GE Healthcare). Monocytes were isolated from PBMCs with anti-CD14 conjugated magnetic microbeads (130-050-201 ...
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bioRxiv - Microbiology 2021Quote: ... Images were analyzed using the Multi Target Analysis Module of the In Cell Analyzer 1000 Workstation Software (GE Healthcare). GFP-positive cells were identified using the top hat segmentation method and counted within the InCell Workstation software.
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I was loaded onto a HisTrap HP column (GE Healthcare Life Sciences). Using an imidazole gradient ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysates containing Hb were initially purified using a Zn2+ charged chelating Sepharose Fast Flow resin column (GE healthcare) because Hb surfaces are rich with His residues that can bind Zn2+ (also see (42)) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell supernatants were collected using 10000 rpm for 10 min and incubated with Glutathione sepharose 4B resin (GE Healthcare) for 2 hr at 4°C ...
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bioRxiv - Cell Biology 2019Quote: Images of the extracellular vesicles and cells were taken with the DeltaVision wide field fluorescent microscope (Applied Precision, GE) outfitted with a digital camera (CoolSNAP HQ ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate is incubated with 100ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) overnight in rotator wheel at 4°C with slow rotation ...
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bioRxiv - Microbiology 2019Quote: Cells extract were subjected to 10% SDS-PAGE and transferred to an Amersham Hybond™-P membrane (GE Healthcare). Membranes were incubated with an HIV-1 p24 monoclonal antibody diluted to 1/1000 (Catalog number 3537) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Media (DMEM, Glibco) supplemented with 10 % Fetal Bovine Serum (FBS, GE Healthcare), 1 % GlutaMAX (Glibco ...
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bioRxiv - Immunology 2021Quote: The purification of 2H1 from NS0 cells consisted of affinity chromatography using rProtein A/Protein G GraviTrap (GE Healthcare). The antibodies were eluted with a 0.1 M glycine-HCL pH 2.7 solution and immediately neutralized with a 1 M Tris-HCl 1 M NaCl pH 9.0 buffer to maintain their stability in solution ...
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bioRxiv - Immunology 2020Quote: ... Secreted Fabs were purified from cell supernatants harvested four days post-transfection using Ni2+-NTA affinity chromatography (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... and purified from clarified transfected cell supernatants four days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Affinity-purified proteins were concentrated and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Immunology 2021Quote: ... IgG from the hybridoma cell line supernatants was purified by affinity chromatography using protein G columns (GE Life Sciences). Purified H5.28 and H5.31 IgG generated from hybridomas was used for EC50 binding measurements ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was pelleted and cell lysate was filtered with a 0.2μm filter and loaded onto a HisTrap Ni column (GE Healthcare) at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Mononuclear cells from synovial fluid and peripheral blood were isolated by density centrifugation using Ficoll-Paque Plus (GE healthcare) and cryopreserved in FBS + 10% DMSO by slow freeze ...
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Microbiology 2022Quote: ... HepG2 nuclei as well intracellular and extracellular sporozoites were counted using the IN Cell Developer Toolbox v.1.9.2 software (GE Healthcare). The area of liver stages was also determined using this software.
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bioRxiv - Immunology 2022Quote: ... Blood cells or buffy coats were diluted in PBS and gently loaded onto the Ficoll-Paque Plus (GE Healthcare) at the volume ratio of 1:1 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA of ARPE-19 cell lines was extracted using the Illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed to SC medium and cells were imaged on a Deltavision widefield fluorescent microscope (GE Healthcare), based on an inverted fluorescence microscope (IX70 ...
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bioRxiv - Cancer Biology 2024Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from blood samples using Percoll (GE Healthcare Life Sciences, Little Chalfont, UK) density gradient centrifugation for DNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... cells were resuspended in Roswell Park Memorial Institute (RPMI) medium and carefully layered on top of Ficoll (GE Healthcare). The upper layer of plasma was carefully removed ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted and subjected to DNaseI treatment using the Illustra RNAspin kit (GE Healthcare). cDNA synthesis from the RNA samples was performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems #4374966 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysate was then 0.45 μm filtered and applied to a 5 mL HisTrap HP column (GE Healthcare). The column was washed with five column volumes of wash buffer ...
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bioRxiv - Genomics 2023Quote: ... Yeast cells were harvested by filtration with 0.45 µM Whatman cellulose nitrate membrane filters (GE Life Sciences #7184-004). Ribosome profiling was conducted as detailed in (McGlincy and Ingolia ...
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bioRxiv - Microbiology 2023Quote: The isolated protist cells were subjected to whole-genome amplification (WGA) using the Illustra GenomiPhi V2 Kit (GE Healthcare) as described previously [37] ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed by centrifugation and proteins were purified from the conditioned media using rProtein A Sepharose (GE healthcare). The purified proteins were buffer-exchanged into a formulation buffer (20 mM His-HAc ...
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bioRxiv - Cancer Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare, Chicago, Il, USA). T cells were negatively purified with EasySepTM beads (human T-cell isolation kit ...
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bioRxiv - Neuroscience 2023Quote: HMC3 cells were grown on 150 mm plates using EMEM (ATCC) supplemented with 10% bovine calf serum (GE Healthcare) and Pen/Strep (Wisent) ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs (Peripheral Blood Mononuclear Cells) were isolated from whole blood samples by Ficoll-Paque density gradient centrifugation (GE Healthcare) and collected ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood by density gradient centrifugation using Ficoll-Paque (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lysate was clarified by centrifugation and purified by FPLC using Ni-NTA (HisTrap FF column, GE Healthcare) followed by size exclusion chromatography (HiLoad 26/60 Superdex 75 ...
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bioRxiv - Neuroscience 2024Quote: ... and numbers and intensity of pSer129 immunofluorescence staining were counted and quantified by IN Cell Developer Toolbox (GE Healthcare). The nuclear count was calculated using the Hoechst count ...
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bioRxiv - Biophysics 2020Quote: ... Cell supernatant were collected after centrifuge at 40,000 rpm for 30 min and loaded onto superpose 6 increase (GE healthcare) for FSEC analysis.
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... High-throughput imaging and quantification of fluorescence signals were carried out using the In Cell Analyzer 2000 (GE Healthcare, USA). ACE2 signal intensity was normalized to total cell number within each well as measured by DAPI staining ...
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bioRxiv - Microbiology 2022Quote: ... Cells were separated from supernatant by centrifugation and supernatant containing the secreted proteins was loaded onto a NiFF (GE Healthcare) column as described above.