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Citations for GE Life Sciences :
3101 - 3150 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The precipitated proteins were purified based on nickel affinity purification by AKTA Prime Plus automated purification system (GE Healthcare). The sample passed over a 1 mL Nickel-Sepharose (HisTrap HP ...
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bioRxiv - Cancer Biology 2023Quote: ... and IgGs were then purified from the culture supernatants by Protein A-column chromatography (GE Healthcare, 17-1279-01). We obtained 6 clones as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed by centrifugation and proteins were purified from the conditioned media using rProtein A Sepharose (GE healthcare). The purified proteins were buffer-exchanged into a formulation buffer (20 mM His-HAc ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated with SDS-PAGE and subsequently transferred to Amersham Hybond-P PVDF Transfer Membrane (RPN303F, GE Healthcare). Membranes were blocked in 5% milk in Tris-buffered saline with 0.01% Tween 20 and incubated with the antibodies listed in Supplemental Table 4 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μL of protein solution were loaded onto a Superdex 200 HR 10/30 gel filtration column (GE Healthcare) with a flow rate of 0.5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... The untagged protein was purified using gel filtration chromatography (ÄKTA with Superdex-200 increase 10/300 column; GE Healthcare) and equilibrated with storage buffer ...
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bioRxiv - Immunology 2023Quote: ... Unpurified proteins contained in HEK293F filtered supernatants were captured on PGT145-functionalized CNBr-activated sepharose 4B beads (GE Healthcare) by overnight rolling incubation at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: Histone proteins were mixed in stoichiometric amounts and eluted through a HiLoad 16/600 Superdex 200pg column (GE Healthcare) to produce H2A-H2B dimers and (H3-H4)2 tetramers separately 40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted for 80 min (350 mA, room temperature) onto Amersham Protran 0.2 μm nitrocellulose membranes (GE Healthcare). Membranes were blocked in 5% milk in TBS-Tween for at least 2 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein samples (4mg/mL; 80 µL) were separated on Superdex 200 Increase 10/300 GL Tricoedn column (GE Healthcare) in 20 mM Hepes containing 150 mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 column (Cytiva, formerly GE Life Sciences) in buffer B ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were further purified via size exclusion chromatography using a Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein in the supernatant was diluted to 1% Triton and incubate with Glutathione Sepharose beads (GE Healthcare) in a batch mode ...
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bioRxiv - Plant Biology 2023Quote: ... Protein bands were quantified by densitometry with ImageQuant TL software (version 7.0 GE Healthcare Life Sciences, Pittsburgh, PA, USA). PsbS abundance was quantified relative to WT based on a dilution series of WT PsbS protein.
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 10/300 GL column (GE Healthcare) and eluted in 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
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bioRxiv - Neuroscience 2023Quote: ... the protein solution was concentrated and further purified utilizing size exclusion chromatography with a SuperdexTM 75 column (GE Healthcare). The protein solution was concentrated to 9.2 mg/mL and used for crystallization.
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bioRxiv - Microbiology 2023Quote: ... Fractions containing predominantly ATeam protein were pooled and applied to a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated with 20 mM tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... A875 cell extracts were incubated for 16 hr at 4°C on a rotating wheel with 0.8μg of anti-p75NTR antibody (ANT-007, Alomone) and then incubated with Sepharose protein-G beads (GE Healthcare). The beads were collected by brief centrifugation (2 min ...
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bioRxiv - Biochemistry 2023Quote: ... The protein fractions were concentrated and finally subjected to Superdex 75 10/300 size exclusion chromatography column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration using a Superose 6 Increase 10/300 GL column (GE Healthcare). Snu71 fragments with residues 1-96 ...
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bioRxiv - Cell Biology 2023Quote: ... soluble proteins were applied to the size exclusion gel filtration column (Superdex 200 Increase 10/300 GL, GE Healthcare), using an Akta P-920 Pump FPLC system (Amersham ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was further purified using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta pure system (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... N-myc proteins were polished using size-exclusion chromatography (SEC) using a Superdex 16/600 S75 column (GE Healthcare). GB1-tagged N-myc proteins were purified in a similar manner with a few alterations ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were incubated with 30 µL of protein G Sepharose™ 4 Fast Flow (#17-0618-01, GE Healthcare) under agitation for 4 hours at 4°C ...
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Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... Protein was purified as previously described [59] but with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) for size exclusion chromatography (SEC) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ml protein was loaded onto the analytical size exclusion column Superdex 200 increase 10/300 GL (GE Healthcare) in running buffer (25 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected after 6 days and purified using a 5-ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5-ml kappa-select column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Deglycosylated proteins were purified to homogeneity by size exclusion chromatography using a HiLoad Superdex 75 PG column (GE Healthcare) equilibrated in Dulbecco’s Phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2024Quote: ... was used to visualize proteins on an Amersham Imager 600 RGB gel and membrane imager (GE Life Sciences/Cytiva).
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to 0.45 µm pore-size nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked at room temperature for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2024Quote: ... Purification was carried out following manufacturer’s instructions on HiTrap Protein G HP purification column for IgG mAbs (GE Healthcare), and a POROS CaptureSelect IgM-XL Affinity Matrix Column (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Refolded HLA-G2 protein was purified by size exclusion chromatography using HiLoad 26/60 Superdex75 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml of pooled mouse sera was then used to purify IgG using Protein A Sepharose resin (GE Healthcare) and digested for using papain-agarose resin (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... 500 µL sample was injected and run with a flow rate of 0.5 mL/min on an ÄKTA Pure protein purification system (GE Healthcare). Control samples containing ThT alone at the lowest (0.4 M urea ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were finally purified using size exclusion chromatography using a Superdex 200 column (10/300 GL, GE Healthcare, Germany) equilibrated with PBS buffer ...
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bioRxiv - Cell Biology 2020Quote: Fresh embryo protein extracts (4 mg in 2.5 ml RIPA buffer) was passed through PD-10 desalting column (GE Healthcare) to remove excess free biotin using the gravity protocol according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was concentrated using Amicon-3.5K filters and subjected to size exclusion chromatography on a Superdex 200 column (GE Healthcare). The concentration of eluted proteins was measured at 280 nm using a Nanodrop spectrophotometer and the proteins were verified by Coomassie Blue staining and western blotting ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the protein were concentrated and gel filtered on a Superdex 75 increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by staining with Coomassie Brilliant Blue R250 or by transferring to a PVDF membrane (GE Life Sciences), and staining with Ponceau S dye or detection by immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions with >95% pure protein were pooled and re-purified from HiTrap Heparin HP affinity columns (GE Healthcare Life sciences) using a linear gradient of 0 – 300 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...