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Citations for GE Life Sciences :
3201 - 3250 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by SEC (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A with pH 7.5 as eluent ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then purified by affinity chromatography using Ni-NTA agarose resin and Glutathione Sepharose 4B resin (GE Healthcare), respectively.
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bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA) affinity column (GE Healthcare) to remove leached residual PGT145-affinity ligand from the previous column ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was expressed using ExpiCHO expression system (LifeTechnologies) according to the manufacturer’s protocol and purified using a Protein A MabSelect SuRE column (GE Healthcare). The wash buffer contained 20mM Tris & 150mM NaCl buffered to pH 8.6 and the elution was done using 0.1 M Citric acid pH 2.5 ...
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bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... followed by transfer of proteins from gel to polyvinylidene fluoride (PVDF) membrane (Amersham Hybond P 0.45, GE Healthcare Life Sciences) and sequential use of primary and secondary antibodies that are listed in Table 3 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by sizeexclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer B with 0.06% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex200 16/60 HiLoad gel filtration column (GE Healthcare). For CLGltPh ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against phospho-Ser935 LRRK2 rabbit monoclonal (1/1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 10% Tris-Tricine gels and imaged using a Typhoon 9410 Molecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Neuroscience 2022Quote: ... We performed size exclusion chromatography on the eluted proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
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bioRxiv - Neuroscience 2022Quote: ... we performed size exclusion chromatography on the concentrated proteins in a Superdex 200 Increase 10/300 GL column (GE Healthcare) using an Akta Ettan fast protein liquid chromatography (FPLC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by size exclusion chromatography on HiLoad 26/600 Superdex 200 prep grade column (GE Healthcare) equilibrated with the working buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: ... and passed through a 0.45-μm filter prior to protein purification via affinity chromatography using an AKTA Start FPLC system (GE Healthcare). The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... TEV protease was added during dialysis to remove the N-terminal His6-tag.The protein was retrieved from dialysis and loaded onto a 1ml HiTrap SP HP IEX column (GE Healthcare) and eluted by gradient introduction of high-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biophysics 2022Quote: ... Protein eluted from the affinity columns was further purified by size-exclusion chromatography using a Superdex 75 column (GE healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Rac1 protein went through a nickel affinity column followed by a Resource Q column and finally Superdex 75 (GE Healthcare) before concentration to 25mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Antibody supernatants were harvested seven days following transfection and purified using protein G affinity chromatography following the manufacturer’s protocol (GE healthcare). In order to validate the functional relevance of Arg100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins (500 µl) were loaded on Superdex 75 10/300 GL or Superdex 200 10/300 GL columns (GE Healthcare) and eluted with buffer 20 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... CM were collected after 48h of transfection and filtered through 0.45 μm syringe filters and used for protein purification via ÄKTA pure 25 FPLC (GE Healthcare) equipped with 1-ml HisTrap affinity column (HisTrap HP ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) in elution buffer (40 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... After gentle stirring for one hour aggregates were removed by centrifugation at 75,600g and the solubilised membrane proteins were applied to a StrepTrap column (GE healthcare) which was then washed in TBS supplemented with 0.01% LMNG ...
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bioRxiv - Immunology 2020Quote: ... IgGs were purified from ∼1-3 ml of serum using equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted off the resin with 0.1 M glycine pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2020Quote: ... labelled protein samples were subjected to size exclusion chromatography at 4°C (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM phosphate (pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... protein samples were concentrated and further purified by preparative SEC using a HiLoad 26/600 Superdex 200 pg (GE Healthcare) column ...
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bioRxiv - Immunology 2021Quote: ... the protein bands on SDS-PAGE gels were scanned and quantified by laser densitometry (Personal Densitometer SI; GE Healthcare, IL). The densities of the Hoc ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Resuspended protein was filtered using a 0.1 um pore size centrifugal filter and loaded onto a Sephadex S200 column (GE Healthcare) for size exclusion chromatography (SEC) ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Cell Biology 2022Quote: ... Bio-Rad (4–15%) gradient gels were used for gel electrophoresis Proteins were blotted onto 0.2 µm nitrocellulose membranes (Amersham Protran, GE Healthcare) using the Thermo Scientific Pierce G2 Fast Blotter ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a Superdex 200 10/300 GL column using an ÄKTA chromatography system (GE Healthcare Life Sciences). 0.5 ml or 1 ml fractions were collected and analyzed by Western blotting using antibodies against HOIP and V5.
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein solution is again filtered before being loaded onto an equilibrated Superdex 200 size exclusion column (GE Healthcare) to separate the FMRP constructs from the Ulp protease and the His-SUMO fusion tag ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized by chemiluminescence (#RPN2232 ECL and #RPN2235 ECL select Western blotting detection reagent – GE Healthcare, Little Chalfont, U.K.) and quantified using ImageLab software v ...
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bioRxiv - Developmental Biology 2022Quote: ... About 500-1,000 resonance units of each of the biotinylated proteins were immobilized on a SA sensor chip (GE Healthcare), using a Biacore S200 machine (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... the protein sample was concentrated and exchanged with phosphate buffer saline (PBS, pH 7.5) using a PD10 column (GE Healthcare) and stored at -80°C ...