Labshake search
Citations for GE Life Sciences :
3251 - 3300 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: The purified rhGFAT2-his protein was subjected to a size exclusion chromatography using a Superdex 200 column (GE Healthcare, USA). The column was equilibrated with 1 CV of 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare). Proteins were extensively washed with Strep buffer (20 mM Hepes ...
-
bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Eluted protein was dialyzed against storage buffer and applied to a HiTrap Q HP anion exchange column (GE Life sciences) pre-equilibrated in buffer A (50 mM HEPES-KOH pH 7.9 ...
-
bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2019Quote: ... and the eluted protein was further purified by size-exclusion chromatography on HiLoad Superdex 200 16/60 column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Genetics 2019Quote: ... Lysate was clarified by centrifugation at 25,000xg and soluble protein was purified using Co2+ affinity chromatography (TALON column, GE Healthcare) followed by anion-exchange chromatography (Q-FF ...
-
bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a hydrophobic HiTrap Butyl HP 1 ml column (GE healthcare life sciences), pre-equilibrated with 5 column volumes of buffer AButyl (500 mM ammonium sulfate ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins into rehydration buffer were incubated with an 18 cm immobilized pH 3–11 nonlinear gradient dry strip (GE Healthcare) for 16 hours at 20°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted proteins were further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences). Purified proteins were concentrated by ultrafiltration prior to flash freezing with liquid nitrogen.
-
bioRxiv - Cell Biology 2019Quote: ... and for additional 4 hours with 40 µl of pre-washed Protein G Sepharose 4 Fast Flow beads (GE Healthcare) in a rotating wheel ...
-
bioRxiv - Molecular Biology 2019Quote: ... and then the eluate was incubated overnight on a rotator at 4°C.The protein was resolved on a HiLoad 16/600 Superdex 200 Column (GE Healthcare) pre-equilibrated with storage buffer (20 mM HEPES-KOH ...
-
bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
-
bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... MnCl2 or CaCl2was titrated into a sample cell containing 0.1 mM catalytic domain of the g12777 protein using an iTC200 calorimeter (GE Healthcare).
-
bioRxiv - Biophysics 2020Quote: ... the proteins were first purified from bacterial cell lysates using His-Trap HP (Ni-NTA) columns (GE Healthcare Life Sciences), as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein then was purified by ion-exchange chromatography through a HiPrep FF Q-column (20 mL, GE Healthcare; FPLC). After the column was equilibrated and washed with Buffer C ...
-
bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: Analytical size exclusion chromatography was performed with 20μl protein samples loaded on a Superdex 200 Increase 10/300 GL column (GE Healthcare) mounted on an Agilent 1260 HPLC ...
-
bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were concentrated ,applied to gel-filtration using a Superdex 75 prep grade HiLoad 16/60 column (GE Healthcare) equilibrated in buffer K (10 mM Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
-
bioRxiv - Microbiology 2020Quote: ... The tag-less protein was then purified by size-exclusion chromatography (SEC) using a Superdex 200 increase column (GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
-
bioRxiv - Microbiology 2020Quote: ... The protein content during the washing and elution steps was monitored using the Äkta purifier 100 system (GE Healthcare, UK) and double-checked with a Bradford assay ...
-
bioRxiv - Microbiology 2021Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer (50 mM NaH2PO4 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Pure proteins with the correct homooligomeric conformation were collected from a Superose 6 10/300 GL SEC column (GE Healthcare) in Tris-buffer (TBS ...
-
bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
-
bioRxiv - Biochemistry 2020Quote: ... Ab Spin Trap columns (immobilized protein G) and NHS-activated sepharose® 4 fast flow were purchased from GE Healthcare Life Sciences (Buckinghamshire ...
-
bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein-containing fractions were then concentrated by microfiltration before loading on a Superdex 200 26/60 prepgrade column (GE Healthcare) column that had been equilibrated in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
-
bioRxiv - Biophysics 2019Quote: ... the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 Column (GE Life Sciences).
-
bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
-
bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... The proteins were further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
-
bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
-
bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 μL solution of 1.5 mg/mL protein was injected onto a Superdex 200 Increase 10/300 GL (GE Healthcare) column equilibrated in a buffer of 20 mM Tris ...
-
bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected and the antibodies were purified on a HiTrap Protein-A column (GE healthcare, Little Chalfont, UK).
-
bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were pooled and 1.6 ml protein was subjected to size exclusion chromatography using a Superdex 200 XK16/60 column (GE Healthcare) and equilibrated into 50 mM Tris-HCL pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
-
bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by size exclusion chromatography (Hi-load 16/60 Superdex 200 column, GE Healthcare Life Sciences) in a buffer (20 mM Tris-HCl ...
-
bioRxiv - Biophysics 2020Quote: ... S-Trimers were purified to homogeneity by consecutive chromatographic steps including Protein A affinity column using MabSelect PrismA (GE Healthcare) which was preloaded with Endo180-Fc at (3 mg/mL ...
-
bioRxiv - Biochemistry 2021Quote: ... anti-EGFR antibody (#MI-12-1, MBL), anti-GFP antibody (#M-048-3, MBL) and Protein A-Sepharose (GE Healthcare) were used for immunoprecipitation ...
-
bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
-
bioRxiv - Microbiology 2020Quote: ... the harvested protein was concentrated to approximately 2.0 ml and filtered using a Superdex 200 gel filtration column (GE Healthcare) equilibrated with buffer (20 mM Tris-HCl and 200 mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...