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Citations for GE Life Sciences :
2951 - 3000 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by size-exclusion chromatography through a HiLoad 26/60 Superdex 200 column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biophysics 2020Quote: ... SEC-MALS was performed at room temperature by passing 500 μl protein (12μM) at 0.3 ml/min flow rate through a Superdex S200 SEC column (GE Healthcare) pre-equilibrated with 10mM potassium phosphate buffer supplemented with 150mM NaCl and 2mM β-mercaptoethanol (pH 8.0) ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Cell Biology 2021Quote: Tag-free syndapin I and III were generated by expressing both proteins in the pGEX-6P vector (GE Healthcare) and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ Premium 0.45 μm NC, GE Healthcare Life sciences, 10600003). For Msb2p-HA and Hkr1p-HA blots ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by ion-exchange chromatography using the HiTrap Heparin HP column (GE Healthcare Life Sciences) and a Mono Q anion exchange column (16/10 ...
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bioRxiv - Biochemistry 2022Quote: ... The untagged protein was concentrated and further purified on a Superdex 200 16/600 gel filtration column (GE Healthcare) in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biochemistry 2022Quote: ... Then the interest protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were cleaved overnight with 3C protease followed by gel filtration on Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (Amersham™ Protran™ 0.2 µm NC, GE Healthcare Life science) using a standard blotting procedure (80V for 2h ...
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bioRxiv - Biochemistry 2022Quote: ... after which protein-containing fractions were pooled and loaded onto pre-equilibrated Superdex 200 Increase 10/300 (GE Healthcare) for size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Proteins released in the soluble form were purified using Glutathione Sepharose 4B according to the manufacturer’s instructions (GE Healthcare). On-column thrombin digestion was performed to release the protein without the GST tag ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using 15% gels and transferred onto nitrocellulose membranes (Amersham Protran, GE Healthcare, USA). Immunoblots were processed by enhanced chemiluminescence (ECL ...
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bioRxiv - Biophysics 2022Quote: ... All the eluted proteins were loaded into Superdex 200 pg or Superdex 75 pg size exclusion chromatography (GE Healthcare) to remove aggregates and potential contaminants.
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bioRxiv - Immunology 2022Quote: ... 5% Glucose and the proteins purified by cation-exchange chromatography using a HiTrap SP High-Performance columns (GE Healthcare), followed by size exclusion chromatography using a HiLoad 16/600 Superdex 75pg column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were further purified by size-exclusion chromatography using an S200 Increase or a Superose S6 column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2020Quote: ... protein containing eluate was concentrated and applied to a HiLoad™ 16/600 Superdex™ 75pg column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2021Quote: ... and the proteins were visualized by enhanced chemiluminescence (ECL) using the ECL Prime Western blotting detection reagent (GE Healthcare) and the ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... the protein of interest was purified by ion-exchange chromatography using MonoQ 4.6/100 PE column (GE Healthcare, Poland) with a linear gradient of 0-100% 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing the protein was concentrated and applied to a SuperdexTM 200 10/30 GL column (GE Healthcare) and eluted with Buffer F (25 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20 mM citric acid pH 5.0 ...
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bioRxiv - Biophysics 2020Quote: ... Protein was purified by Ni2+ affinity chromatography followed by size-exclusion chromatography on a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected and filtered using 0.22µm membrane filters and loaded onto HiTrap MabSelect SuRe Protein A (GE Healthcare) column pre-equilibrated against PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Extracts (15⍰μg protein) were separated by SDS–PAGE and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked in TBS (150⍰mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: Differential scanning calorimetry (DSC) was carried out on the S proteins using a MicroCal VP-DSC instrument (GE Healthcare, MicroCal Products Group ...
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bioRxiv - Immunology 2020Quote: ... The protein was then further purified by size exclusion chromatography using a Superdex 75 10/300 column (GE Healthcare). Ramos B cells expressing VRC01 germline B cell receptor were provided by Daniel Lingwood (Ragon Institute).
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bioRxiv - Microbiology 2021Quote: ... Twenty-five µL of Protein A Sepharose beads (50% slurry contained phosphate-buffered saline buffer) (GE healthcare, Chicago, IL) was added to the resultant supernatant ...
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bioRxiv - Biochemistry 2021Quote: Expressed CYP168A1 was purified using fast protein liquid chromatography with a HisTrap-HP affinity column (GE Healthcare, Chicago, IL). The bacterial cell pellets were thawed on ice and resuspended in buffer A consisting of 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... all proteins were further purified using size-exclusion chromatography on a Superdex-200 column (GE Healthcare, Buckinghamshire, United Kingdom). Their corresponding elution profiles are shown in Figure S12 ...
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bioRxiv - Bioengineering 2020Quote: The eluted proteins were concentrated and subjected to size-exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in the PBS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were typically resolved on 7.5% TGX stain-free gels and transferred onto nitrocellulose membrane (GE Healthcare, Amersham Protran) using wet-transfer at 100 V for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 µm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg, GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were first purified with Ni Agarose 6 FF (AOGMA) with the AKTA system (GE Healthcare Life Sciences) and further purified with anti-Flag affinity beads ...
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bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was dialyzed against storage buffer and applied to a HiTrap Q HP anion exchange column (GE Life sciences) pre-equilibrated in buffer (50 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...