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Citations for GE Life Sciences :
2901 - 2950 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred (100 V, 1 hr) onto PVDF (polyvinylidene difluoride) membranes (GE Healthcare Life Sciences) using 1X Tris/Glycine buffer (in-house 50X solution containing 250 mM Tris and 1920 mM Glycine) ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Further purification method was carried out with a 1 mL HisTrap HP column (GE Healthcare, Sweden) to obtain the pure protein ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2h at RT with streptavidin-HRP (1:400, GE Healthcare UK). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on a 5 ml HisTrap™HP column (1 mL/min; GE Healthcare) in a binding buffer containing 100 mM tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) and purified with GST-BIN1_SH3 bound to Glutathione-Sepharose 4B beads (GE Healthcare). Human SH3 of BIN1 with GST tag (GST-SH3 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
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bioRxiv - Genomics 2024Quote: ... SERBP1 immunoreactivity was detected with a horseradish peroxidase– conjugated anti-mouse IgG (1:5000, GE Healthcare). GAPDH immunoreactivity was detected using an anti-mouse IgG (1:6000 ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with Anti-mouse HPR-conjugated secondary antibody (1:5000 dilution, GE Healthcare) at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) was exchanged to a storage buffer omitting DTT using a PD10 (GE Healthcare) gel filtration column ...
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bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... and filtered onto 1 μm polycarbonate membrane filters (142 mm diameter, Whatman/GE Healthcare, Uppsala, Sweden) representing the viral fraction ...
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bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody was detected with HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-liked sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Biochemistry 2023Quote: ... the solubilized enzymes were loaded onto a 1 mL Q-sepharose HP column (GE Healthcare, UK) pre-equilibrated in buffer containing 20[mM Tris-HCl (pH 7.14 at 20[°C) ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized using Radiance Plus chemiluminescent HRP substrate (Azure Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000; GE Healthcare; NA943V). Membrane was visualized as described above.
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bioRxiv - Cell Biology 2022Quote: ... and cloned into BamHI and NotI restriction sites within the pGEX-4T-1 plasmid (GE Healthcare), such that the gene was in frame with an N-terminal GST tag (pGEX-4T-1-GST-KIN-EC-ter) ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted sample was then applied to a 1 mL HiTrap Q HP column (GE Healthcare), and UBXD1 was eluted with a 0-50% gradient of AEX elution buffer (AEX binding buffer supplemented with 1000 mM KCl) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered (Filter paper, Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), a 1 mL aliquot of the filtrate was transferred into a 1.5 mL centrifuge tube ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cpxA31-163 was cloned into BamHI/EcoRI-digested pGEX-6P-1 expression vector (GE Healthcare), which encodes for an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on a 1 ml HisTrap HP Q column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were probed with an ECL anti-rabbit IgG secondary antibody (1:10,000) from GE Healthcare Life Science and imaged using Chemidoc Molecular Imaging System (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and applied to a Superdex 200 10/300 size exclusion column (GE Healthcare) that was pre-equilibrated with cross-linking buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: The adjusted pool was loaded onto a 1 ml Phenyl-HP hydrophobic interaction column (GE Healthcare) pre-equilibrated with PepA Buffer D then washed with the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... The crude PepA solution was loaded onto a HiTrap Heparin-HP 1 ml column (GE Healthcare), then washed with PepA Buffer A [PepA Buffer C without Glycerol] ...
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bioRxiv - Biophysics 2024Quote: ... then by a Heparin-affinity purification using a 1 ml HiTrap Heparin-HP column (GE Healthcare). Other FPLC steps from Subramanya et al ...
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bioRxiv - Cell Biology 2024Quote: ... at 37 °C for 1 h and alkylated with 15 mM IAA (GE Healthcare, Mississauga, ON) in the dark at room temperature for 30 min followed by quenching with 15 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies were used as follows: Horseradish peroxidase (HRP)-conjugated mouse IgG (1:10,000; GE Healthcare) or rabbit IgG (1:3000 ...
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bioRxiv - Biophysics 2021Quote: ... cell debris was removed and the supernatant loaded onto a 10 ml volume nickel-sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2020Quote: ... were added at 25ul/ml to exclude immune and endothelial cells using MagRack 6 (GE Healthcare 89129-096). Cells were either frozen for future use or stained for flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: An IN Cell Analyzer 6000 automated fluorescence microscope (cat. 29-0433-23, GE Healthcare Bio-Sciences, Marlborough, MA) was used for imaging cells ...
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bioRxiv - Cell Biology 2020Quote: ... Live-cell imaging were performed using a DeltaVision RT wide-field fluorescence imaging system (Applied Precision, Issaquah, WA) equipped with a quantifiable laser module ...
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bioRxiv - Genetics 2021Quote: ... and RNA was extracted from cells using a magnetic bead-based protocol using Sera-Mag SpeedBeadsTM (GE Healthcare). Two libraries were prepared per clone using SMARTseqv4 kit (Clonetech) ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Cell Biology 2022Quote: ... and 3T3 cells were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (GE Healthcare), 100 U/ml penicillin and streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Images for immunolabelled cells on micropatterns were acquired with an inverted wide field Deltavision Core Microscope (Applied Precision) equipped with highly sensitive cooled interlined charge-coupled device (CCD ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Cells were strained through a 70 μm filter and separated on a discontinuous 40%/80% Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2019Quote: ... fixed and subjected to high content microscopy in a IN Cell Analyzer 6000(GE healthcare life sciences, USA).
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bioRxiv - Neuroscience 2019Quote: ... Mononuclear cells were isolated from CB and PB by density centrifugation using standard Ficoll-Hypaque technique (GE Healthcare) then treated with 0.15M NH4Cl to lyse residual erythrocytes and washed in phosphate-buffered saline (PBS).
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bioRxiv - Genetics 2020Quote: ... Fluorescently labeled glial cells were scored visually across optical stacks with a Deltavision Core imaging system (Applied Precision) and a PlanApo 60x/1.42 NA oil immersion objective.
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from cells using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
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bioRxiv - Physiology 2021Quote: ... The stained myotubes were imaged at 10–20X magnification on the IN Cell Analyzer 6000 platform (GE Healthcare). For each treatment condition ...
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bioRxiv - Cancer Biology 2020Quote: ... Mononuclear cells (MNCs) were isolated by density gradient separation (Ficoll-Paque PREMIUM; GE Healthcare, Little Chalfont, Buckinghamshire, UK) and immediately analyzed or vitally frozen for later use ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...