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Citations for GE Life Sciences :
2851 - 2900 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Protein containing fractions were pooled and buffer exchange was carried out using a PD-10 column (GE Healthcare) using TES-KCl buffer (25 mM TES/KOH pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS/PAGE in 10% gel before wet transfer to 0.45μm nitrocellulose membranes (GE Healthcare) and blocked in 5% dry milk powder in TBS-T (100mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing purified proteins were concentrated and further purified by a Superdex 200 size-exclusion column (GE Healthcare) equilibrated in buffer containing in the 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: The protein was further purified by size exclusion chromatography (SEC) (Superdex 200 Increase 10/300 GL, GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... S17 were prepared by blotting tau and α-synuclein proteins onto nitrocellulose membrane (GE Healthcare Life Science, 10600004). Human HT40 fibrils (Stressmarq ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrated protein sample was loaded on a pre-equilibrated Superdex-75 16/60 column (GE Healthcare, USA). A buffer consisting of 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... the target protein was further purified using a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare). The size-exclusion column buffer contained 50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to a nitrocellulose membrane with a 0.2 μm pore size (GE Healthcare Life Sciences, 10600001) by wet transfer at 100 V for 120 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluate was then applied to an ion exchange chromatography column (2x 1ml HiTrap Q HP, GE Healthcare) equilibrated with QA buffer ...
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bioRxiv - Immunology 2024Quote: ... Proteins were separated on 12% SDS polyacrylamide gels and transferred to nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membranes were blocked in 5% dried milk/TBS-T buffer and incubated over night with anti-mouse TREX1 antibody (clone C-11 ...
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bioRxiv - Biochemistry 2024Quote: ... and the flowthrough containing phosphorylated proteins was purified over Hiload 16/600 Superdex 75 pg column (GE Healthcare) preequilibrated with buffer containing 25 mM Tris (pH 7.5) ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing target protein were pooled and incubated over night at 4°C with PreScission protease (GE Healthcare) at a ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... the protease was removed from (un)cleaved target protein using a HisTrap FF column (GE Healthcare Life Sciences). Remaining nucleic acids were removed using anion exchange chromatography (AIEX ...
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bioRxiv - Biophysics 2024Quote: ... Protein was concentrated and inject into size-exclusion chromatography on a Superdex 16/60 75 column (GE Healthcare) pre-equilibrated in buffer 20 mM Bis-Tris pH 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein bands were visualized and imaged by using an ImageQuant LAS 500 scanner (GE Healthcare, Chicago, IL, USA). After transfer ...
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bioRxiv - Microbiology 2024Quote: ... thawed protein samples were purified by size-exclusion chromatography using a Superdex 200 (16/600) column (GE Healthcare) in equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: Proteins were purified by immobilized metal-affinity chromatography (IMAC) on a HisTrap HP nickel-Sepharose column (GE Healthcare) at 4°C following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Elution fractions containing the most protein were combined and loaded directly onto a size-exclusion column (GE Healthcare) pre-equilibrated with lysis buffer without prior concentration or dialysis of the protein ...
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bioRxiv - Microbiology 2024Quote: ... All protein lysates were separated on 10% polyacrylamide gels and blotted on polyvinyl difluoride membranes (PVDF, GE Healthcare). Blotted membranes were blocked with either 5% non-fat milk or 5% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein solutions was loaded on to Superose 6 Increase 10/300 GL column (GE Healthcare, Madison, WI) that was equilibrated with a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Immunology 2024Quote: ... The concentrated protein was injected into a Superdex 200 Increase 10/300 GL (GE Healthcare 28-9909-44) size exclusion column chromatography system (Waters) ...
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bioRxiv - Microbiology 2020Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was filtered (0.45 µm) and further purified by size-exclusion chromatography (Superdex S200 26/60; GE Healthcare) in buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with corresponding antibody and chemiluminescence was visualized according to the manufacturer’s instructions (ECL Advanced; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and proteins were visualized using the with Image-Quant LAS 4000 biomolecular imager (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: The concentrated protein was then applied to a HiPrep 26/60 Sephacryl S-100 column (DEAE FF) (GE healthcare) which was equilibrated with the re-suspension buffer supplemented with 100 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The labelled protein was further purified by size-exclusion chromatography using Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The cleaved protein was then concentrated to 2.0 ml final concentration and loaded on the S200 column (GE healthcare). S200 size exclusion chromatography has yielded a single peak around 230 KDa ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-chromatin complexes were purified with Protein A conjugated to sepharose beads (GE Healthcare, 30 µL GE17-5280-01) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The ion-exchange chromatography was performed for further protein purification with an anion-exchange column (Source 15Q, GE Healthcare). Then peak fractions were collected and stored at -80 °C before using.
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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bioRxiv - Immunology 2019Quote: ... after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane, GE Healthcare Life Sciences) or PVDF membranes (Amersham Hybond P 0.45 µm PVDF Membrane ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified on an ion exchange column MonoS and gel filtration column Superose 6 (GE Healthcare). Purified protein was concentrated to ∼20 mg/ml in BRB20 buffer (20mM PIPES ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2019Quote: ... Protein at 50 µM was injected onto a Superdex 200 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... 40 μg of total protein were run on a SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (GE Healthcare). After blocking in 5% (w/v ...
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bioRxiv - Immunology 2019Quote: ... All His-tagged proteins were purified from the cell culture medium using HisTrap Excel column (GE Healthcare, catalog 17371206) and eluted using buffer containing 50 mM HEPES-NaOH ...