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Citations for GE Life Sciences :
51 - 100 of 2205 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Plant Biology 2021Quote: ... α-P4 or α-P6 before subsequent addition of secondary horseradish peroxidase (HPR)-conjugated antibodies (1:20.000; Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Molecular Biology 2023Quote: ... or α rabbit (GE Healthcare, Life Sciences NA934V) HRP conjugated antibodies were used ...
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bioRxiv - Microbiology 2023Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... Radioactive signal was detected on a Typhoon 9 500 FLA scanner (GE Healthcare). Quantification of the EtBr and radioactive signals was performed with Fiji software (80) ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Molecular Biology 2021Quote: ... non-α-gal allergic donor (α-gal sIgE <0.10) were isolated using a Ficoll–Paque gradient (GE Healthcare, Chicago, IL, USA). Endogenous IgE was stripped from basophils within the PBMC fraction by incubating the cells with cold lactic acid buffer (13.4 mM lactic acid ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Biophysics 2021Quote: ... 8 mM of NHS-Cy5 (GE Healthcare) or NHS-Sulfo-Cyanine 7 (Lumiprobe ...
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bioRxiv - Cell Biology 2020Quote: ... peroxidase-linked sheep α-mouse IgG (GE Healthcare, #NA931V) and AMDEX goat α-rabbit IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... and AMDEX goat α-rabbit IgG (GE Healthcare, RPN4301) was used ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were: α-Mouse-HRP (GE Healthcare), α-Rabbit-HRP (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... either secondary α se (GE Healthcare, Life Sciences NA931) or α rabbit (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... probed with a 1:2,000 dilution of polyclonal α-PotA (Lin et al., 2017) followed by a 1:10000 dilution of peroxidase labeled α-mouse (GE Healthcare) and developed with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: A total of 1.5 mg each of 0 hr and 3 or 5 hr ssDNA samples were labeled with Cy3-dUTP or Cy5-dUTP (GE Healthcare) by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... The α-syn oligomers and free α-syn from the supernatant were separated using a Superdex 200 Increase 10/300 GL (GE Healthcare, 28990944) equilibrated with PBS ...
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bioRxiv - Biophysics 2022Quote: ... and anti–α-catenin shRNA (α-cat-KD) (both courtesy of G.D. Longmore, Washington University) were cultured in DMEM/F12 (GE Healthcare Life Sciences), supplemented with 5 % horse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM β-mercaptoethanol) on an ÅKTA system (GE Healthcare). PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... mouse anti-β-tubulin 1:8000 (13-8000, GE Healthcare), mouse anti-Repo 1:20 (8D12 ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were Cy3-labeled α-mouse (GE Healthcare), fluorescein-conjugated α-rat (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: The blots were subsequently washed 3 x 5 min in TBST 1X and developed with the Prime Western Blotting detection Reagent (GE Healthcare Amersham) using the LAS3000 Bioimager (Fujifilm ...
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bioRxiv - Neuroscience 2021Quote: ... was analyzed by electrophoresis on a denaturing 8% polyacrylamide gel containing 8 M urea followed by transfer to a positively-charge Hybond nylon membrane (GE Healthcare). Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol ...
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bioRxiv - Molecular Biology 2023Quote: ... Stock Percoll was prepared by combining 9 parts of Percoll (cat# 17-0891-02, GE healthcare) with 1 part of clear (without phenol red ...
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bioRxiv - Immunology 2024Quote: ... were sourced from a healthy donor with no α-gal allergies (α-gal sIgE <0.10) and isolated through a Ficoll–Paque (GE Healthcare in Chicago, IL, USA) gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM β-mercaptoethanol) using gel-filtration NAP column (GE Healthcare) and concentrated by ultrafiltration to 1.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... secondary α-mouse-HRP conjugated antibody (GE Healthcare, Life Sciences NA931) was used ...
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bioRxiv - Biochemistry 2020Quote: ... and quantified using ImageQuant TL 8 software (GE Healthcare). Comparative analysis of numerous tRNA and enzyme variants was performed using single-turnover rate conditions with the concentration of Hs ProXp-ala several fold smaller than the apparent Kd ...
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bioRxiv - Biochemistry 2019Quote: ... PreScission proteinase (8 units/mg of protein, GE Healthcare) was added to remove the His6 tag from the Asf1 ...
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bioRxiv - Neuroscience 2024Quote: Samples were loaded on 9% polyacrylamide gels and then transferred onto nitrocellulose membranes (0.22 μm, GE Healthcare). Membranes were blocked in 5% milk ...
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bioRxiv - Neuroscience 2021Quote: ... air dried and exposed to Hyper film β-max (GE Healthcare, USA) for seven days ...
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bioRxiv - Synthetic Biology 2022Quote: ... and PAA-β-galactose (Lectinity) were immobilized on CM5 chips (GE Healthcare) pre-coated with streptavidin ...