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Citations for GE Life Sciences :
2801 - 2850 of 3164 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... All fractions identified to contain the cleaved protein were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer (20 mM Bis-Tris pH 7.0 ...
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bioRxiv - Biophysics 2019Quote: ... Before experiments 1 mL of aSyn was further purified using a Superdex 75 pg 10/300 GL size exclusion chromatography (SEC) column (GE Healthcare) to obtain monomeric protein ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a cation exchange HiTrap SP HP 5 ml column (GE healthcare life sciences) pre-equilibrated with 5 column volumes of buffer ASP (100 mM Ammonium acetate ...
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bioRxiv - Biochemistry 2019Quote: ... 0.1 ml of this solution was subjected to SEC at 293 K on a Superdex 200 10/300 column (GE Healthcare) preequilibrated in 10 mM MES pH 6.0 ...
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bioRxiv - Systems Biology 2020Quote: ... Whole blood was diluted with room temperature RPMI medium in a 1:2 ratio to facilitate cell separation for other analyses using the SepMate PBMC isolation tubes (STEMCELL) containing 16 mL Ficoll (GE Healthcare). Diluted whole blood was centrifuged at 1200 g for 20 minutes at 20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µL of a 10 mg/mL mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare) were added to each sample ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... concentrated using centrifugal filters to bring down the volume to ∼1 mL and injected onto a Superdex 200 16/60 size exclusion column (GE Healthcare) previously equilibrated in HEPES 50 mM ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was incubated at 37°C for 2-3 hours on an orbital shaker and loaded onto an anion-exchange colum (1 ml DEAE FF, GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Biochemistry 2020Quote: ... Eluate from GSH affinity chromatography was subjected to heparin affinity chromatography using a HiTrap HP Heparin column (5 mL GE Healthcare) equilibrated with NMR buffer (10 mM MES ...
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bioRxiv - Biophysics 2020Quote: ... which was maintained at a constant flow rate of 0.7 mL/min using an AKTA Pure FPLC (GE Healthcare Life Sciences) and the eluate after it passed through the UV monitor was directed through the SAXS flow cell ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions containing LPR1 (detected by immune blot analysis) were directly applied to cation exchange carboxymethyl-sepharose column (HiTrap CM FF, 1-ml, GE Healthcare) equilibrated with buffer B (20 mM Na2HPO4-NaH2PO4 ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Biochemistry 2020Quote: ... The elute was concentrated to 5 ml and applied to a gel filtration run on a 16-600 HiLoad Superdex 200 pg column (GE Healthcare), preequilibrated with 20 mM Hepes (pH 7.0) ...
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bioRxiv - Microbiology 2020Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble His6-M protein was purified on an Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were topped up to 1 ml with Buffer A and loaded onto a HiPrep 16/60 Sephacryl S-500 HR column (GE Healthcare). Buffer A was run at 1 ml/min and the flow-through was collected in 5 ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... the primary antibody was visualized using 2~5 μg/ml secondary antibody towards specific species of primary antibody labeled with Cy3B (GE Healthcare) or Atto 488 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins (500 μl) at a concentration of 1 mg/ml were loaded on a Superdex 75 10/300 GL column (GE Healthcare) and eluted with HEPES buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... to a volume of 2 ml and applied to size exclusion chromatography (SEC; HiLoad 26/600 Superdex 200 pg, GE Healthcare), After SEC ...
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bioRxiv - Microbiology 2020Quote: ... using pE-SUMO as an expression plasmid (86) and was isolated using a 1-mL HisTrap column and Äkta start (GE Healthcare). Before using the fusion protein for far western blotting ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The fractions corresponding to the second peak in the chromatogram (elution volume 70-75 ml) were pooled and subjected to further size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After concentration by spin filtration to 4-5 mL pro-rhodesain was loaded onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), and purified by SEC (0.5 mL/min ...
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bioRxiv - Plant Biology 2020Quote: ... 400 μl of purified protein at 1.4 mg·ml−1 was fractionated on a Superdex 200 10/300 column equilibrated in GF buffer using an AKTA purifier (GE Healthcare) at a flow rate of 0.5 ml·min−1 ...
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bioRxiv - Biochemistry 2021Quote: ... 1mM CaCl2 (pH 8.0)) at a flow rate of 1 mL/min on an ÄKTA-Pure instrument (GE Healthcare, Piscataway, NJ). The purified proteins were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were analysed using SDS-PAGE and peak fractions were collected and further purified using a 1 ml MonoQ column (GE Healthcare). Sic1 was eluted with 20 column volumes (CV ...
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bioRxiv - Cell Biology 2021Quote: ... The purified recombinant proteins Spn2-BFP or BFP were concentrated to 2 ml and were further purified by SEC with a HiLoadTM 16/600 superdexTM 200 pg column (28-9893-35; GE Healthcare) in SEC buffer (50 mM Tris (pH 7.5) ...
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bioRxiv - Immunology 2020Quote: ... FP3 or FP4 from the Mono S column were pooled and further purified by SEC performed on a 24 mL Superdex 200 increase column (GE Healthcare) at 4 °C with a flow rate of 0.25 mL/min in a buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: ... 100 µL of sample at 15 µM was injected from a static loop beginning at the 0 ml point of each run onto a Superdex 200 increase 10/300 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2019Quote: ... A 50 – 500 mM KCl gradient was used to elute IF2 from a 5 ml Hi-Trap SP HP column (17115201, GE Healthcare). Fractions were analysed by 8% acrylamide SDS-PAGE and those containing IF2 were pooled together ...
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bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... Then the peak fractions were desalted and further purified by ion-exchange chromatography using 1-ml Resource S column (GE Healthcare) in buffer E (20 mM mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... 360562 RCF spin in a Ti70 rotor and incubated with 2 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare) for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Cell Biology 2019Quote: Human his-tagged TRMT6/61A dimer was purified from Escherichia coli BL21 cells using 1 mL HisTrap HP affinity chromatography columns (GE Healthcare). Eluted fractions containing the recombinant proteins were pooled and incubated overnight with PreScission protease at 4°C ...
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA/PBS and incubated with Cy3-conjugated or FITC-conjugated goat anti-rabbit IgG (10 mg/ml; GE Healthcare) for 45 min respectively ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM NaCl to a final concentration of ∼3 mg/ml further purified by size-exclusion chromatography using a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... containing 0.05 M NaCl to a final concentration of 1 mg/ml was applied to a Superdex 10/300 column (GE Healthcare) run at a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2021Quote: The eluent from the Ni-NTA purification of the expression product of the pET-Duet-1 vector in BL21(DE3)pLysS* cells was loaded onto a 24 ml Superdex-200 10/300 GL (GE Healthcare) column on an AKTA Purifier-10 workstation ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of protein sample diluted to a concentration of between 0.02 mg/ml and 0.05 mg/ml was added to a grid and then manually blotted away with Whatman Grade 1 filter Paper (GE Healthcare). 3 µl of 0.75% Uranyl Formate was then added to the grid and blotted away ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using our previously reported protocols.17 TNKS2 constructs were cloned into pNIC-MBP expression vectors and an additional purification on an MBPTrap HP 5 ml column (GE Healthcare) prior to cleavage with TEV protease was performed.20 Details of the constructs for each PARP enzyme are listed in Table S1.