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Citations for GE Life Sciences :
2901 - 2950 of 3164 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were obtained 24 hours after treatment and GST was purified on a 1 mL GSTrap HP column (GE Healthcare) with an AKTA purifier 10 FPLC system ...
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bioRxiv - Molecular Biology 2023Quote: ... Imidazole and NaCl present in the Ni-NTA running buffer were eliminated by using a HiTrap Desalting 5 mL (GE Healthcare) column.
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was filtered and loaded onto a 5 mL HisTrap HP column (Cytiva) using an AKTA Start (GE Healthcare). The column was washed in buffer containing 50 mM Tris-HCl (pH 8) ...
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bioRxiv - Immunology 2022Quote: ... His-tagged Fabs were prepared by transient transfection of truncated heavy chain genes encoding a C-terminal 6x-His tag with a light chain expression vector and purified from supernatants using a 5 mL HisTrap colum (GE Healthcare) followed by SEC as described above.
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on two 5 mL-HiTrap heparin columns (GE Healthcare) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL) in a chemically defined media (ActiPro media; GE Healthcare, USA). The cultures were maintained with setpoints for pH at 7.2±0.05 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions were pooled and purified by anion-exchange on a 5 ml Hitrap Q (Cytiva, formerly GE Healthcare Life science) in a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Molecular Biology 2023Quote: ... Further protein purification was achieved by performing size-exclusion separation of 5 mL of the eluate from the affinity chromatography step on a Hiload 16/60 Superdex S200 column (GE Healthcare), with the use of 1.2 column volumes of gel-filtration (GF ...
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bioRxiv - Microbiology 2023Quote: ... The TEV digested proteins were separated from the cleaved His-tag product on an AKTA Pure 25L FPLC using a 5 mL HisTrap-FF with the captured flowthrough further purified using a HiLoad Superdex 75 PG gel filtration column (GE Healthcare). A monodisperse peak was obtained and assessed for purity using SDS-PAGE gel analysis ...
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bioRxiv - Microbiology 2023Quote: ... PmScsC-EcDsbA was initially purified by immobilized metal affinity chromatography (IMAC) using a 5 mL HisTrap Fast Flow column (GE Healthcare) followed by cleavage of the N-terminal His6-tag with TEV protease and reverse IMAC ...
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 50 mM NaCl and loaded on an equilibrated 5 ml HiTrap Heparin HP Sepharose column (GE Healthcare). The protein was eluted using a linear 0–1 M NaCl gradient in buffer B ...
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... injected (5 mL total) into HiLoad 16/600 Superdex 75pg (Cytiva) connected to an AKTA FPLC system (GE Healthcare Life Science). Gel filtration was performed using the same buffer to purify Nurr1-RXRα LBD heterodimer for analysis ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2023Quote: ... The solution was concentrated to ∼1 ml and went through gel filtration on Superdex 75 10/300 increase column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions from ion-exchange chromatography were filtered through a 5 µm syringe filter and loaded onto a 22 mL Source 15ISO hydrophobic interaction column (GE Healthcare) equilibrated in buffer C (50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The protein was purified by ion exchange chromatography using DEAE sepharose followed by size exclusion chromatography using a 60 ml Superdex 75 increase column (GE healthcare) in 6M GuHCl ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... The soluble fraction was filtered through a 0.45 μm sterile syringe filter and loaded onto a pre-equilibrated 5 mL HiTrap IMAC HP column (GE Healthcare Biosciences). The column was washed with 4 column volumes of lysis buffer followed by 4 column volumes of wash buffer 1 [50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was then concentrated in an Amicon Ultra-15 50,000 MWCO spin concentrator to ∼10-20 mg/mL and loaded onto a HiPrep 26/60 S200 Size Exclusion Column (GE Healthcare), equilibrated in Buffer D ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Cell Biology 2023Quote: ... and pure fractions were pooled and concentrated before being concentrated and snap-frozen in liquid nitrogen GST-tagged RAB7A was similarly isolated via passing cleared lysate over a gravity column packed with 5 mL of Glutathione Sepharose 4B (GE Healthcare), followed by elution using 10 mM reduced glutathione dissolved in 50 mM Tris-HCl pH 8.0. ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets were resuspended in 6 mL 25 % Standard Isotonic Percoll (Percoll: GE Healthcare, 17-5445-02 with 10% 10x PBS) in Medium A ...
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bioRxiv - Biochemistry 2023Quote: ... Final purification and concentration of the untagged DAP5 was performed using a 1 ml HiTrap heparin HP column from GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
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bioRxiv - Biophysics 2023Quote: ... Q7 or Q20 at a concentration of ~4-7mg/ml were loaded onto an S200 Increase size-exclusion column (Superdex 200 Increase10/300 GL, GE Healthcare) at a flow rate of 0.5 ml min-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Capturing of ORF1p-His6 protein was performed using Immobilized-Metal Affinity Chromatography (IMAC) by loading of 7M Urea Extract on 5 mL HisTrap FF FPLC column (GE Healthcare) at 0.5 mL/minute flow rate followed by washing with 10 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Biochemistry 2023Quote: GB1-FKBP-ICD and GB1-FRB-ICD were mixed at equimolar ratio and loaded onto a 24-mL Superdex 200 column (GE healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then subjected to size exclusion chromatography in 20 mM tris pH 8.0 using a Superdex-75 HR 10/30 column fractionating at a flow rate of 0.5 mL/min and attached either to an AKTA FPLC system (GE Healthcare, Buckinghamshire) or a BioLogic DuoFlow system (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fractions were removed by centrifugation at 20000 g during 30 min at 4°C and the supernatant was loaded on a 1 mL HisTrapTM HP column (GE Healthcare) connected to a NGC chromatography system (BioRad) ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Microbiology 2023Quote: ... with a 10 kDa cut-off and loaded onto a Superdex 200 pg HiLoad 16/600 (GE Healthcare, 1 mL/min). Sample purity was assessed by 12% SDS-PAGE analysis and sample concentration was determined by spectrophotometry using a theoretical molar extinction coefficient at 280 nm of 20 ...