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Citations for GE Life Sciences :
2601 - 2650 of 3164 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Histones were then further purified by cation exchange on a 5 ml HiTrap SP HP column (GE Healthcare Life Sciences), pre-equilibrated with HGFB150 (full-length histones and tailless H2B) ...
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bioRxiv - Microbiology 2022Quote: ... coli supernatant was directly applied onto a 1-ml Ni2+ ion chelating poly-histidine affinity column (HisTrap HP, GE Healthcare) equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to ice cold 15 ml tubes containing 1ml of IgG Sepharose 6 Fast Flow (GE Healthcare) and incubated on a rotating platform for 3 hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant containing the 6xHis-tagged DMTases was stirred with 6 mL of NiCl2-charged chelating Sepharose beads (GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2 μm) and loaded with a flow rate of 1 ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Plant Biology 2022Quote: ... Additional purification was carried out by anion-exchange chromatography on a 1 mL HiTrap Q Sepharose column (GE Healthcare, USA). Yields of the recombinant-produced proenzymes ranged from 0.1 to 2 mg per litre culture ...
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Biochemistry 2023Quote: ... The eluent was pooled and concentrated to a volume of ∼0.5 mL with a 30-kDa Vivaspin concentrator (GE Healthcare) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered (0.45 polyvinylidene fluoride membrane, Carl Roth, Germany) and applied onto a 1 ml Nickel-IDA column (GE Healthcare) equilibrated in 50 mM TRIS-HCl pH 7.8 ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biochemistry 2023Quote: Protein samples within the concentration range 0.5-1 mg/mL were injected onto pre-equilibrated Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in buffer containing 20 mM Tris-Cl ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was concentrated to a total volume of 2.5 mL and desalted using PD-10 desalting columns (GE Healthcare) and desalting buffer (25 mM Tricine-NaOH ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration with a flow rate of 0.8 mL/min using FPLC systems (AKTA pure 25, GE Healthcare Life Science). The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Subsequently the elutes were concentrated and applied to a 120 mL HiLoad 16/600 Superdex 75 column (GE Healthcare, Inc.) equilibrated with buffer B (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... MlaFEDB-containing fractions eluted from the NiNTA column were pooled and concentrated to 0.5 mL before separation on a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer (10 mM Na2HPO4·7H2O ...
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bioRxiv - Microbiology 2023Quote: The supernatant was filtered with a 0.22 μm filter and was loaded onto a pre-equilibrated HisTrap HP 1 ml column (GE Healthcare) at 0.5 ml/min with a peristaltic pump at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged at 40,000 rcf for 1 h and the supernatant was filtered through a 0.45 μM syringe filter (Chromafil GF/PET 45, MachereyNagel) and passed through a HiTrap SP HP (5 mL, GE Healthcare) column connected to an NGC FPLC system (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... Soluble lysate was filtered through a 0.45 µm syringe filter and loaded onto a 140 mL Q Sepharose FastFlow ion-exchange column (GE Healthcare) equilibrated in buffer A (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 and equilibrated with wash buffer (50 mM K-HEPES pH7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Immunology 2023Quote: ... Blots were then wash 3 times in 15-30 mL TBST (0.05% v/v) and chemiluminescence were quantified on the Amersham Imager 680 (GE Healthcare). All immunoblots shown are representative of 3 or more biological replicates.
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bioRxiv - Biochemistry 2023Quote: ... The elution was concentrated (<2.5 mL) and applied to a HiLoad® 16/60 Superdex® 200 pg (GE Healthcare) size-exclusion column ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... were injected at flow rate of 0.4 ml min−1 to a Superdex 75 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer containing 20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein monomers were separated from oligomers by size exclusion chromatography on a Superdex 200 column (120 mL, GE Healthcare) equilibrated with buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Soluble lysate was filtered through a 0.45 µm syringe filter and loaded onto a 140 mL Q Sepharose FastFlow ion-exchange column (GE Healthcare) equilibrated in buffer A (50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... Acidified soluble lysate was filtered through a 0.45 µm syringe filter and loaded onto a 125 mL SP Sepharose FastFlow ion-exchange column (GE Healthcare) equilibrated in buffer A (50 mM NaOAc ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... CypA was purified by subtractive anion exchange chromatography using a 10 mL HiTrap Q HP column (GE Healthcare Life Science) equilibrated with 25 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... and lysed by resuspension in 20 ml cold F Buffer (18% w/v Ficoll-PM400 (GE Healthcare 17-0300-50), 40 mM potassium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... The column was run at a flow rate of 0.5 mL min-1 on an AKTAbasic liquid chromatography system (GE Healthcare) coupled to a miniDawn MALS detector and an Optilab refractive index detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 ml of purified WT SAM domain at 9.5 mg/ml was loaded onto a Superdex 75 16/60 gel filtration column (GE Healthcare) at 1 ml/min pre-equilibrated in buffer D ...
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bioRxiv - Biochemistry 2024Quote: ... nanobody was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5 ml of base buffer was then applied to PD10 columns to elute the nanobody protein base buffer without imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... protein was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5ml of base buffer was then applied to PD10 columns to elute the RECQL5 protein in base buffer ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample (0.5 mL final volume) was loaded onto a Superdex 200 increase 10/300 size exclusion column (GE Healthcare) at a flow rate of 0.4 mL/min and the absorbance at 280 nm was monitored ...
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bioRxiv - Biochemistry 2024Quote: ... 50-100 μL of the purified protein samples (concentration ranging 2-15mg/ml) were filtered (with 0.1 μm pore size) and loaded onto a Superdex200 10/300GL column (GE Healthcare) preequilibrated with SEC buffer ...
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL of GTL25D1/COLGALT1 concentrated at 4 mg/mL were injected into a Superdex 200 PC 3.2/300 Increase column (GE Healthcare), pre-equilibrated with 25 mM HEPES/NaOH ...