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Citations for GE Life Sciences :
2701 - 2750 of 3164 citations for Dechlorane Plus Syn Bis Cyclopentene 13C10 99% 100 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... was accomplished using 0.5 M imidazole and fractionated into 1 ml aliquots using the Frac30 fraction collector (GE Life Sciences # 29023051). Fluorescence of FAST proteins was checked via brief excitation (Figure 2D ...
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bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2019Quote: ... the cell suspension diluted in 1 mL of complete L15 medium was carefully layered on top of 5 mL of Percoll gradient (L15/10 mM HEPES/20% Percoll (GE Healthcare) and centrifuged at 400 g for 9 min ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
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bioRxiv - Biochemistry 2020Quote: ... Tag and TEV protease removal was achieved by re-loading the dialyzed sample onto the 5 mL HisTrap Excel column (GE Healthcare), and collecting the unbound tagless sample ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest (NT-mini or NT*-mini) was then eluted into a 5 ml Ni-IMAC HisTrap Excel column (GE Healthcare) by further increasing the NaCl concentration to 600 mM (buffer NT.B) ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated to 2 mL and further purified by size-exclusion chromatography using HiLoad 16/600 Superdex 200 (Ge healthcare).
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bioRxiv - Microbiology 2021Quote: ... ParBΔCTD-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been pre-equilibrated with 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2020Quote: A solution of 25 mg/L SC-SWCNTs and 2 mg/mL C16-PEG2k-ceramide in 1% sodium cholate was added to a 1 kDa molecular weight cutoff dialysis cartridge (GE Healthcare). The solution was dialyzed against water with multiple water exchanges for a week ...
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Biochemistry 2021Quote: ... The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column into 20 mM NaPO4 150 mM NaCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was concentrated to 5 ml and loaded in a HiLoad 16/600 Superdex 75pg gel-filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... LPMO containing fractions were pooled and concentrated to 1 mL before being loaded onto a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) operated with a running buffer consisting of 50 mM Tris pH 7.5 and 200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were purified by loading the supernatant onto a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) equilibrated with 5 CV of 95% Buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... primary mouse hepatocytes isolated via the two-step collagenase perfusion method were suspended in 1 ml DMEM media and subjected to Percoll (GE Healthcare) gradient centrifugation at 1,000g for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Cleaved protein was passed through a 5 ml HisTrapFF column and further purified by size-exclusion chromatography (S200 16/60 column; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Soluble RNA was separated from solid acrylamide by filtration through 0.22 micron filters and then manually applied to a 5 mL HiTrapQ column (GE Healthcare # 29051325) that had been equilibrated in buffer D (300 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to less than 1 mL and was injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 293-F cells were seeded 2-3 h before transfection at a density of 1×106 cells per ml in Freestyle medium supplemented with 0.2% w/v HyClone Cell Boost (GE Healthcare, SH30584.02). For transient transfection ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were clarified by centrifugation (20 minutes at 18,000x g) and bound to a 5 mL HisTrap™ FF Crude column (GE Healthcare) and eluted with 50-75 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... 4°C for 30 minutes and loaded onto a 5 ml HighTrap MBP column (GE Healtcare) using an Äkta-Pure system (GE Healthcare). The column was washed with lysis buffer and protein eluted with 10 column volumes MBP-elution buffer (100 mM Tris-HCl pH 7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation (40000 g for 30 min) and the supernatant applied onto a 5 ml StrepTrap column (GE Healthcare). After washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... The dialyzed DCX sample was centrifuged to remove any precipitates before loading onto a Strep-Trap (1 mL, cat. No. 29-0486-53, GE Healthcare) column ...
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bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Plant Biology 2021Quote: ... using an AKTA Pure 25 system equipped with HisTrap HP 1 mL column and submitted to gel filtration using a Superdex 200 increase 10/300 GL column (GE healthcare). Purified recombinant proteins were conserved at −80°C in 1x PBS solution containing 10% glycerol.
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bioRxiv - Plant Biology 2021Quote: ... Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare) to remove the excess of copper ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biophysics 2021Quote: ... The relevant fractions were pooled and concentrated to 10-20 mg/mL for loading on a Superdex 200 Increase 10/300 (GE Healthcare) size exclusion chromatography column equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The buffer was then adjusted to contain 40 mM imidazole and the rNAs were purified with a HisTrapTM FF 1 ml column (GE Healthcare) using an Akta Start protein purification system (Cytiva) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to a gravity flow chromatography column packed with 1.5 mL of Ni Sepharose 6™ fast flow resin (GE healthcare) and incubated at 4 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was then centrifuged at 37,500 g for 30 min and the supernatant incubated with 5 mL GFP nanobody-coupled CNBr-activated Sepharose resin (GE Healthcare) for 2 hrs (Kubala et al. ...
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bioRxiv - Biophysics 2021Quote: ... The dialyzed protein was then either frozen in 2 mL aliquots at −80°C until needed or immediately further purified from aggregates by size exclusion chromatography (Superdex S75; GE Healthcare) in SEC buffer ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl and 10 mM β-ME and loaded onto a 5 ml HiTrap SP anion exchange column (GE Healthcare). The binary complex ScNatC was then eluted using a salt gradient (50–750 mM NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... 100-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 200 or 75 10/300 GL Increase size-exclusion chromatography column (GE Healthcare) in 20 mM Tris HCl ...